Long-term stability studies of fluorocarbon oxygen transport emulsions

Johnson, O.L.; Washington, C.; Davis, S.S.

doi:10.1016/0378-5173(90)90102-a

Cited by 13 publications

(10 citation statements)

References 3 publications

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“…The long-term stability test further showed that FDC emulsions prepared with F127 and F68 were stable for more than 3 months (Figure c,d). In previously reported Pluronics-stabilized FDC nano-sized emulsions, the addition of soya oil was indispensable for preventing significant size variation during autoclaving. , However, a higher stability was achieved in our emulsions without any additives. Suppressing a large size variation because of emulsion instabilities can prevent embolism on administration and size distribution-related toxicity .…”

Section: Resultsmentioning

confidence: 81%

“…In previously reported Pluronics-stabilized FDC nano-sized emulsions, the addition of soya oil was indispensable for preventing significant size variation during autoclaving. 40,41 However, a higher stability was achieved in our emulsions without any additives. Suppressing a large size variation because of emulsion instabilities can prevent embolism on administration and size distribution-related toxicity.…”

Section: ■ Results and Discussionmentioning

confidence: 84%

“…Suppressing a large size variation because of emulsion instabilities can prevent embolism on administration and size distribution-related toxicity. 41 Furthermore, results shown in Figures 3 and 4 are useful for a comprehensive understanding of how Pluronic copolymers affect the function of emulsions as OCs. Because of good biocompatibility 42 and high stability as shown above, 2% F127-stabilized emulsions were chosen as a representative formulation and used for the following experiments unless stated.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Size-Controlled Preparation of Microsized Perfluorocarbon Emulsions as Oxygen Carriers via the Shirasu Porous Glass Membrane Emulsification Technique

Ohta

Kamihira

et al. 2019

Langmuir

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We have developed microsized perfluorocarbon (PFC) emulsions with different sizes as artificial oxygen carriers (OCs) via Shirasu porous glass membrane emulsification. Monodispersed PFC emulsions with narrow size distribution were obtained. By changing the membrane pore size, we were able to precisely control the size of emulsions and fabricate emulsions similar in size to human red blood cells. Behaviors of Pluronics with different physiochemical properties (F127, F68, P85, and P103) as surfactants were also investigated, which evidenced that the type and concentration of Pluronics have a major impact on the size of emulsions and the response to different thermal conditions. The F127-stabilized microsized PFC emulsions were stable even during autoclave sterilization. The emulsions were loaded with Ru(ddp)an oxygen-sensitive probeon their surfaces to indicate oxygen concentration. Finally, incubations with HeLa cells that show fluorescence in response to hypoxia cultured in 2D and 3D suggested promising potential of our emulsions for OCs.

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Section: Resultsmentioning

confidence: 81%

Section: ■ Results and Discussionmentioning

confidence: 84%

Section: ■ Results and Discussionmentioning

confidence: 99%

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Size-Controlled Preparation of Microsized Perfluorocarbon Emulsions as Oxygen Carriers via the Shirasu Porous Glass Membrane Emulsification Technique

Ohta

Kamihira

et al. 2019

Langmuir

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“…Briefly the perfluorodecalin oil (20% w/v) was homogenized in the block copolymer solution (4% w/v) using a small high-pressure homogenizer (Microfluidizer, Microfluidics Inc., Newton, MA) operating at 15 000 psi. Ostwald ripening was controlled by adding 4% w/v perfluoroperhydrophenanthrene, a high-boiling oil which was completely miscible with perfluorodecalin. − The emulsion droplets were washed and transferred to a 72% D 2 O/28% H 2 O mixture by repeated centrifugation. Dynamic light scattering (Malvern 4700 photon correlation system) indicated a volume mean diameter of 270 nm and a polydispersity of 0.05.…”

Section: Methodsmentioning

confidence: 99%

Effect of Electrolytes and Temperature on the Structure of a Poly(ethylene oxide)−Poly(propylene oxide)− Poly(ethylene oxide) Block Copolymer Adsorbed to a Perfluorocarbon Emulsion

Washington

King

1997

Langmuir

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We have used small-angle neutron scattering to study the detailed structure of the poly(ethylene oxide)−poly(propylene oxide)−poly(ethylene oxide) (PEO−PPO−PEO) block copolymer Poloxamer 188 adsorbed at the interface in a perfluorodecalin in water emulsion and specifically to investigate the effect of electrolytes and of temperature. Low (<0.5 M) concentrations of 1:1 electrolyte caused the polymer layer to extend from the interface, apparently by desorption of loop contacts between the PEO chains and the perfluorocarbon−water interface. The extension of the polymer layer is accompanied by a reduction in the fraction of bound polymer segments. A similar effect was found with increasing temperatures. Higher concentrations of electrolyte caused the PEO chains to collapse slightly toward the surface, and it is suspected that this was due to the water becoming a poorer solvent for PEO as the ionic strength of the dispersion medium was increased. No corresponding collapse of the polymer chains was observed at higher temperatures, in the temperature range studied (25−55 °C), but this does not rule out collapse at higher temperatures; the cloud point of the polymer in 1% solution is 112 °C.

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“…These emulsions have an intrinsic pharmaceutical application since they are used as oxygen transport media. In particular, the perfluorodecalin−Poloxamer 188 system has been widely studied as “artificial blood” for clinical use. − Since water and perfluorocarbons have similar Hamaker constants (they have almost identical refractive indices), these emulsions are easily stabilized, even by weakly adsorbing polymers. This allows stable emulsions with droplet diameters from 100 to 300 nm to be easily prepared by high-shear homogenization.…”

Section: Introductionmentioning

confidence: 99%

Structure of Block Copolymers Adsorbed to Perfluorocarbon Emulsions

1996

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We have used small-angle neutron scattering (SANS) to study the structure of block copolymers (Poloxamer 188, Poloxamer 407, and Poloxamine 908) adsorbed at the perfluorodecalin−water interface in a submicrometer emulsion system. The data were analyzed using an established transform method to yield the polymer volume fraction profile as a function of distance from the oil−water interface. There was a good correlation between the derived adsorbed amounts of polymer and those measured experimentally, and between the adsorbed layer dimensions from SANS and earlier light scattering measurements. The profiles are consistent with a model in which the poly(propylene oxide) blocks are bound at the interface and the poly(ethylene oxide) blocks are largely present as tails. An electrolyte (sodium chloride at 0.1−0.2 M concentration) was found to significantly reduce the bound fraction of Poloxamer 188, and caused a significant increase in the extension of the adsorbed layer, without affecting the amount of polymer adsorbed.

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Long-term stability studies of fluorocarbon oxygen transport emulsions

Cited by 13 publications

References 3 publications

Size-Controlled Preparation of Microsized Perfluorocarbon Emulsions as Oxygen Carriers via the Shirasu Porous Glass Membrane Emulsification Technique

Size-Controlled Preparation of Microsized Perfluorocarbon Emulsions as Oxygen Carriers via the Shirasu Porous Glass Membrane Emulsification Technique

Effect of Electrolytes and Temperature on the Structure of a Poly(ethylene oxide)−Poly(propylene oxide)− Poly(ethylene oxide) Block Copolymer Adsorbed to a Perfluorocarbon Emulsion

Structure of Block Copolymers Adsorbed to Perfluorocarbon Emulsions

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