Long-term results of linaclotide in the treatment of constipation-type irritable bowel syndrome

Martínez, Fernando Geijo; Garrido, A; Prieto, Héctor Marcos; Pérez, Concepción Piñeiro; Bermejo, Ana Beatriz Prieto; Delgado, Alberto Álvarez; Guardado, A Velasco; Pérez, Antonio Rodríguez

doi:10.17235/reed.2018.5268/2017

Cited by 1 publication

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…............. (1) ITR: intestinal transit rate; D: distance traveled by the ink (in cm); IL: intestinal length (in cm). All measurements were performed in triplicate.…”

Section: Assessment Of Intestinal Transit Timementioning

confidence: 99%

See 1 more Smart Citation

Effect of atractylenolide III on interstitial cells of Cajal and C-kit/SCF pathway of rats with loperamide-induced slow transit constipation

Wang¹,

Yuxia²,

Li³

et al. 2021

Trop. J. Pharm Res

View full text Add to dashboard Cite

Purpose: To determine the effect of atractylenolide-III (ATL-III) on loperamide-induced slow transit constipation (STC) in a rat STC model, and to elucidate the mechanisms involved. Methods: Male Wistar rats were divided into five groups (n=6 per group): normal control group (NG), model group, and three STC rat groups treated with different doses of ATL-III, viz, 5, 10 and 15 mg/kg. The rats were treated for 15 days. Feed consumption, fecal excretion and intestinal transit rate were determined. Nitric oxide synthase (NOS), somatostatin (SS), serotonin (5-HT), and vasoactive intestinal peptide (VIP) were measured with enzyme-linked immunosorbent assay (ELISA). The protein and mRNA expressions of C-kit, SCF, PKC, and PI-3K were assayed using Western blot analysis and realtime reverse transcription polymerase chain reaction (RT-PCR), respectively. Results: The amount, weight, and moisture content of stool, and water consumption were significantly higher in ATL-III-treated groups than in the untreated (model) group (p < 0.05), whereas no difference was observed in feed intake. Intestinal transit rate was higher in the ATL-III-treated groups (p < 0.05). Decreased NOS, SS and VIP levels and increased 5-HT level were seen in the ATL-III-treated groups (p < 0.05). ATL-III treatment also induced increases in smooth muscle cells, neuronal cells, and mucous layer (p<0.05). Results from RT-PCR and Western blot revealed that ATL-III–treated groups had elevated c-kit, SCF, PKC, as well as PI-3K mRNA and protein expressions (p < 0.05). Conclusion: These results suggest that ATL-III mitigates loperamide-induced STC in rats via stimulation of NOS, SS, VIP, and 5-HT secretions. It also increases smooth muscle cells, neuronal cells, and mucous layer, and regulates the signaling pathways involving PKC, PI3K, SCF, and c-kit.

show abstract

“…............. (1) ITR: intestinal transit rate; D: distance traveled by the ink (in cm); IL: intestinal length (in cm). All measurements were performed in triplicate.…”

Section: Assessment Of Intestinal Transit Timementioning

confidence: 99%

“…Constipation is a global concern of public health, particularly affecting elderly population [1]. Slow transit constipation (STC) is caused by a disorder in colon transmission, and it is characterized by symptoms including infrequent and difficult defecation, accompanying with a sensation of incomplete bowel evacuation.…”

Section: Introductionmentioning

confidence: 99%

Effect of atractylenolide III on interstitial cells of Cajal and C-kit/SCF pathway of rats with loperamide-induced slow transit constipation

Wang¹,

Yuxia²,

Li³

et al. 2021

Trop. J. Pharm Res

View full text Add to dashboard Cite

show abstract

Long-term results of linaclotide in the treatment of constipation-type irritable bowel syndrome

Abstract: long-term linaclotide treatment in patients with C-IBS is effective and safe in the clinical setting.

Cited by 1 publication

References 22 publications

Effect of atractylenolide III on interstitial cells of Cajal and C-kit/SCF pathway of rats with loperamide-induced slow transit constipation

Effect of atractylenolide III on interstitial cells of Cajal and C-kit/SCF pathway of rats with loperamide-induced slow transit constipation

Contact Info

Product

Resources

About