Long-term results of cell-free biodegradable scaffolds for in situ tissue engineering of pulmonary artery in a canine model

Matsumura, Goki; Isayama, Noriko; Matsuda, Shunji; Taki, Kensuke; Sakamoto, Yuki; Ikada, Yoshito; Yamazaki, Kenji

doi:10.1016/j.biomaterials.2013.05.037

Cited by 55 publications

(40 citation statements)

References 13 publications

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“…Overall, our results are in line with those of Matsumura et al who evaluated cell-free scaffolds in the IVC and pulmonary valve locations in dogs and found endothelialization, vascular smooth muscle cell proliferation, and synthesis of an extracellular matrix. 33,34 Such remodeling of the matrix is a key process in vascular restoration and is impacted by the number and polarity of invading monocytes and macrophages. From this standpoint, PDO and PHVBB outperformed PU with regard to the predominance of infiltrating regulatory ED2 macrophages, a phenotype associated with less inflammatory tissue scarring and improved remodelling.…”

Section: Figmentioning

confidence: 99%

Polymer-Based Reconstruction of the Inferior Vena Cava in Rat: Stem Cells or RGD Peptide?

Pontailler

Illangakoon

Williams

et al. 2015

Tissue Engineering Part A

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As part of a program targeted at developing a resorbable valved tube for replacement of the right ventricular outflow tract, we compared three biopolymers (polyurethane [PU], polyhydroxyalkanoate (the poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxyvalerate) [PHBVV]), and polydioxanone [PDO]) and two biofunctionalization techniques (using adipose-derived stem cells [ADSCs] or the arginine-glycine-aspartate [RGD] peptide) in a rat model of partial inferior vena cava (IVC) replacement. Fifty-three Wistar rats first underwent partial replacement of the IVC with an acellular electrospun PDO, PU, or PHBVV patch, and 31 nude rats subsequently underwent the same procedure using a PDO patch biofunctionalized either by ADSC or RGD. Results were assessed both in vitro (proliferation and survival of ADSC seeded onto the different materials) and in vivo by magnetic resonance imaging ( MRI), histology, immunohistochemistry [against markers of vascular cells (von Willebrand factor [vWF], smooth muscle actin [SMA]), and macrophages ([ED1 and ED2] immunostaining)], and enzyme-linked immunosorbent assay (ELISA; for the expression of various cytokines and inducible NO synthase). PDO showed the best in vitro properties. Six weeks after implantation, MRI did not detect significant luminal changes in any group. All biopolymers were evenly lined by vWF-positive cells, but only PDO and PHBVV showed a continuous layer of SMA-positive cells at 3 months. PU patches resulted in a marked granulomatous inflammatory reaction. The ADSC and RGD biofunctionalization yielded similar outcomes. These data confirm the good biocompatibility of PDO and support the concept that appropriately peptide-functionalized polymers may be successfully substituted for cell-loaded materials.

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Section: Figmentioning

confidence: 99%

Polymer-Based Reconstruction of the Inferior Vena Cava in Rat: Stem Cells or RGD Peptide?

Pontailler

Illangakoon

Williams

et al. 2015

Tissue Engineering Part A

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“…Alternatives have been searched in order to produce scaffolds, of them being the use of decellularized tissue-derived scaffolds 8,9 . The advantages of this material are related to the essential elements contained in the organic extracellular matrix, such as collagen, fibrinogen, hyaluronic acid, glycosaminoglycans (GAGs), and hydroxyapatite (HA), which is more conducive to cell adhesion and differentiation 10 .…”

Section: ■ Introductionmentioning

confidence: 99%

Ultrastructural analysis and residual DNA evaluation of rabbit vein scaffold

et al. 2017

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Purpose: To investigate the ultrastructural characteristics and analysis of residual DNA in scaffold models, produced with decellularized vena cava in an experimental model with rabbits. Methods: Three groups were created for ultrastructural and residual DNA analysis: group 1 -control, consisting of samples of vena cava in natura; group 2 -SD, consisting of vein fragments submitted to 2% sodium deoxycholate decellularization by shaking (160rpm -Shaker News Brunswick Scientific ® ) for 1 hour at controlled temperature shaker at 37°C; group 3 -SDS, consisting of vein fragments submitted to 1% sodium dodecyl sulfate decellularization under the same previous condition, for 2 hours. Results: The ultrastructural matrix of the blood vessel maintained its vintegrity after either decellularization models. The results of the two quantification methods demonstrated a significant decrease in the DNA content of the decellularized vena cava samples as compared to the control samples and, differed statistically from each other, p <0.05. Conclusion: The 2% DS protocol for vein decellularization, in this experimental model, was considered the best protocol because it presented less amount of residual DNA without causing substantial destruction of the extracellular matrix.

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“…Again, they reported no significant differences in hydroxyproline and elastin content between the left pulmonary artery and the TEVG; however, significant calcification was observed with the TEVG at 12 months. Although the authors noted a lack of stenosis and thrombosis in the vasculature of any of the animals, the tubular scaffolds were 8 mm in diameter (Matsumura et al, 2013) and may not replicate the hemodynamics of a small-diameter blood vessel.…”

Section: Tissue Engineering Of Vascular Grafts In Vivomentioning

confidence: 99%

Vascular Grafting Strategies in Coronary Intervention

2014

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With the growing need for coronary revascularizations globally, several strategies to restore blood flow to the heart have been explored. Bypassing the atherosclerotic coronary arteries with autologous grafts, synthetic prostheses, and tissue-engineered vascular grafts continue to be evaluated in search of a readily available vascular graft with clinically acceptable outcomes. The development of such a vascular graft including tissue engineering approaches both in situ and in vitro is herein reviewed, facilitating a detailed comparison on the role of seeded cells in vascular graft patency.

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Long-term results of cell-free biodegradable scaffolds for in situ tissue engineering of pulmonary artery in a canine model

Cited by 55 publications

References 13 publications

Polymer-Based Reconstruction of the Inferior Vena Cava in Rat: Stem Cells or RGD Peptide?

Polymer-Based Reconstruction of the Inferior Vena Cava in Rat: Stem Cells or RGD Peptide?

Ultrastructural analysis and residual DNA evaluation of rabbit vein scaffold

Vascular Grafting Strategies in Coronary Intervention

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