Long term recordings with microelectrode arrays: Studies of transcription-dependent neuronal plasticity and axonal regeneration

Hofmann, Frank; Bading, Hilmar

doi:10.1016/j.jphysparis.2005.12.005

Cited by 64 publications

(50 citation statements)

References 78 publications

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“…Mapping LTD in a cortical circuit MEA technology represents a valuable tool to record responses in numerous sites simultaneously for a long time (Oka et al, 1999;Morin et al, 2005;Hofmann and Bading, 2006). We have used a 64-channel MEA system, MED64, in mouse brain slices to study the spatial distribution of synaptic transmission and LTD in the ACC.…”

Section: Discussionmentioning

confidence: 99%

Plasticity of Metabotropic Glutamate Receptor-Dependent Long-Term Depression in the Anterior Cingulate Cortex after Amputation

et al. 2012

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Long-term depression (LTD) is a key form of synaptic plasticity important in learning and information storage in the brain. It has been studied in various cortical regions, including the anterior cingulate cortex (ACC). ACC is a crucial cortical region involved in such emotion-related physiological and pathological conditions as fear memory and chronic pain. In the present study, we used a multielectrode array system to map cingulate LTD in a spatiotemporal manner within the ACC. We found that low-frequency stimulation (1 Hz, 15 min) applied onto deep layer V induced LTD in layers II/III and layers V/VI. Cingulate LTD requires activation of metabotropic glutamate receptors (mGluRs), while L-type voltage-gated calcium channels and NMDA receptors also contribute to its induction. Peripheral amputation of the distal tail impaired ACC LTD, an effect that persisted for at least 2 weeks. The loss of LTD was rescued by priming ACC slices with activation of mGluR1 receptors by coapplying (RS)-3,5-dihydroxyphenylglycine and MPEP, a form of metaplasticity that involved the activation of protein kinase C. Our results provide in vitro evidence of the spatiotemporal properties of ACC LTD in adult mice. We demonstrate that tail amputation causes LTD impairment within the ACC circuit and that this can be rescued by activation of mGluR1.

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Section: Discussionmentioning

confidence: 99%

Plasticity of Metabotropic Glutamate Receptor-Dependent Long-Term Depression in the Anterior Cingulate Cortex after Amputation

et al. 2012

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“…testing and behavioral studies [2,20] . Compared to traditional electrophysiology, the MEA technique is superior in that it enables: (1) gathering large amounts of spatial information on the internal dynamics of networks with multisite recordings [42,43] ; (2) long-term analysis of the spatiotemporal distribution of network-level electrical activity [20,44] ; (3) multisite stimulation and recording within one slice [20,42] ;…”

Section: Examples Of Ltd Recording Using Meas Besidesmentioning

confidence: 99%

“…(4) evaluation of network physiological properties and the pharmacological effects of compounds [16,22,45,46] ; (5) stable recording that is less sensitive to factors such as mechanical vibration [42,43] ; and (6) a tremendous variety of research applications involving acute brain slices [10,16,45,47] , organotypic slice cultures [38,44,46,48] and dissociated cell cultures [7,17] . It is well-suited for studying synaptic plasticity [21,[31][32][33] , single unit activity [49,50] , rhythmic activity [17,45,51] , and pharmacological drug testing [23,46,52] .…”

Section: Examples Of Ltd Recording Using Meas Besidesmentioning

confidence: 99%

Use of multi-electrode array recordings in studies of network synaptic plasticity in both time and space

Liu

Chen

et al. 2012

Neurosci. Bull.

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Simultaneous multisite recording using multi-electrode arrays (MEAs) in cultured and acutely-dissociated brain slices and other tissues is an emerging technique in the field of network electrophysiology. Over the past 40 years, great efforts have been made by both scientists and commercial concerns, to advance this technique. The MEA technique has been widely applied to many regions of the brain, retina, heart and smooth muscle in various studies at the network level.The present review starts from the development of MEA techniques and their uses in brain preparations, and then specifically concentrates on the use of MEA recordings in studies of synaptic plasticity at the network level in both the temporal and spatial domains. Because the MEA technique helps bridge the gap between single-cell recordings and behavioral assays, its wide application will undoubtedly shed light on the mechanisms underlying brain functions and dysfunctions at the network level that remained largely unknown due to the technical difficulties before it matured.

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“…Given the above statements, the aim of the present study was to evaluate potential differences in the induction rate system [34][35][36][37] , with the following advantages: (1) detect the activity of neuronal networks in both space and time [38][39][40][41] ;…”

Section: Introductionmentioning

confidence: 99%

“…(2) record and stimulate the electrophysiological activity of many sites simultaneously [39,40,42,43] ; (3) stable recordings that are less sensitive to factors such as mechanical vibrations [39][40][41] . with ad libitum water and rat chow.…”

Section: Introductionmentioning

confidence: 99%

长串与短串θ节律刺激在大鼠前扣带回皮质脑片诱导长时程增强效应的不同作用: 平面微电极阵列记录技术

2009

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Objective There is substantial evidence supporting the notion that the anterior cingulate cortex (ACC) is an important limbic structure involved in multiple brain functions such as sensory perception, motor conflict monitoring, memory, emotion and cognition. It has been shown that long term potentiation (LTP) is an important synaptic model of neural plasticity in the ACC, however, little is known about the spatiotemporal properties of ACC at network level. The present study was designed to see the LTP induction effects across different layers of the ACC by using different conditioning stimuli (CS) protocols. Methods A unique multi-electrode array recording technique was used in the acutely-dissociated ACC slices of rats. Long and short train theta burst stimulation (TBS) paradigms were applied in layer V-VI as the CS and the LTP induction effects were compared across different layers of the ACC. Briefly, both long and short train TBS are composed of bursts (4 pulses at 100 Hz) with a 200 ms interval, however, the former (TBS1) was with 10 trains and the latter (TBS2) was with 5 trains.After test stimulation at layer V-VI in the ACC, network field potentials (FPs) could be simultaneously recorded across all layers of the ACC. Results The waveforms of FPs were different across different layers. Namely, positive-going waveforms were recorded in layer I and negative-going waveforms were recorded in layers V-VI, in contrast, complex waveforms were localized mainly in layers II-III. Following application of two CS protocols, the induction rate of LTP was significantly different between TBS1 and TBS2 regardless of the spatial properties. TBS1 had more than 60% success, while TBS2 was less than 25% in induction of LTP. Moreover, both the 2 CS protocols could induce LTP in layers II-III and layers V-VI without layer-related difference. However, no LTP was inducible in layer I. Conclusion The present findings indicate that stimulation protocols may, at least in part, account for a large portion of variations among previous LTP studies, and hence highlight the importance of selecting the best LTP induction protocol when designing such experiments. Moreover, the present results demonstrate the prominent superiority of multi-electrode array recording in revealing the network properties of synaptic activities in the ACC, especially in comparing the spatiotemporal characteristics between different layers of this structure.

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Long term recordings with microelectrode arrays: Studies of transcription-dependent neuronal plasticity and axonal regeneration

Cited by 64 publications

References 78 publications

Plasticity of Metabotropic Glutamate Receptor-Dependent Long-Term Depression in the Anterior Cingulate Cortex after Amputation

Plasticity of Metabotropic Glutamate Receptor-Dependent Long-Term Depression in the Anterior Cingulate Cortex after Amputation

Use of multi-electrode array recordings in studies of network synaptic plasticity in both time and space

长串与短串θ节律刺激在大鼠前扣带回皮质脑片诱导长时程增强效应的不同作用: 平面微电极阵列记录技术

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