Long-term, quantitative analysis of gametogenesis in autotriploid rainbow trout, Oncorhynchus mykiss

Carrasco, L. A. P.; Doroshov, Serge I.; Penman, David J.; Bromage, Niall

doi:10.1530/jrf.0.1130197

Cited by 48 publications

(28 citation statements)

References 21 publications

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“…By contrast, control triploid males never produced white-colored milt. It has been reported that triploid male rainbow trout produce small amounts of aneuploid sperm, which cannot fertilize (Carrasco et al, 1998). Therefore, we concluded that the three recipients that produced white-colored milt showed donor-derived spermatogenesis, and these recipients were used for further progeny tests.…”

Section: Research Reportmentioning

confidence: 89%

“…The triploid recipients were produced by suppression of the second polar body extrusion by heatshock treatment. It is widely known that triploid female trout are completely sterile and do not proceed to vitellogenesis (Carrasco et al, 1998). Twenty-month-old triploid recipients that received diploid ovarian germ cells had ovaries that possessed a large colony of vitellogenic oocytes (Fig.…”

Section: Research Reportmentioning

confidence: 99%

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Sexual plasticity of ovarian germ cells in rainbow trout

et al. 2010

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The sexual plasticity of fish gonads declines after the sex differentiation period; however, information about the plasticity of the germ cells themselves after sex differentiation is limited. Using rainbow trout (Oncorhynchus mykiss), we recently established a novel germ cell transplantation system that provides a unique platform with which to dissect the developmental and cellular mechanisms underlying gametogenesis. Using this technique, we show here that transplanted ovarian germ cells isolated from 6- to 9-month-old donors can colonize sexually undifferentiated embryonic gonads and resume gametogenesis. Ovarian germ cells containing oogonia and early oocytes isolated from female rainbow trout were transplanted into the peritoneal cavities of hatching-stage fry of both sexes and the behavior of the donor cells was observed. The transplanted ovarian germ cells migrated towards the recipient gonads, interacted with embryonic gonadal somatic cells, proliferated rapidly, and eventually differentiated into eggs in female recipients and sperm in male recipients. Furthermore, the donor-derived eggs and sperm obtained from the recipient fish were functional and were able to produce normal offspring. These findings indicate that mitotic germ cells, the oogonia, possess a high level of sexual plasticity.

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Section: Research Reportmentioning

confidence: 89%

Section: Research Reportmentioning

confidence: 99%

Sexual plasticity of ovarian germ cells in rainbow trout

et al. 2010

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“…In zebrafish, gametogenesis has not been studied before in triploids, but in rainbow trout (Carrasco et al 1998) and sea bass (Felip et al 2001) it was shown to result in compromised fertility. As expected, we now also see in zebrafish that spermatogenesis is impaired due to disorganized synapsis, which is a logical consequence of the odd chromosome number.…”

Section: à15mentioning

confidence: 99%

Mlh1 Deficiency in Zebrafish Results in Male Sterility and Aneuploid as Well as Triploid Progeny in Females

et al. 2007

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In most eukaryotes, recombination of homologous chromosomes during meiosis is necessary for proper chromosome pairing and subsequent segregation. The molecular mechanisms of meiosis are still relatively unknown, but numerous genes are known to be involved, among which are many mismatch repair genes. One of them, mlh1, colocalizes with presumptive sites of crossing over, but its exact action remains unclear. We studied meiotic processes in a knockout line for mlh1 in zebrafish. Male mlh1 mutants are sterile and display an arrest in spermatogenesis at metaphase I, resulting in increased testis weight due to accumulation of prophase I spermatocytes. In contrast, females are fully fertile, but their progeny shows high rates of dysmorphology and mortality within the first days of development. SNP-based chromosome analysis shows that this is caused by aneuploidy, resulting from meiosis I chromosomal missegregation. Surprisingly, the small percentage of progeny that develops normally has a complete triploid genome, consisting of both sets of maternal and one set of paternal chromosomes. As adults, these triploid fish are infertile males with wild-type appearance. The frequency of triploid progeny of mlh1 mutant females is much higher than could be expected for random chromosome segregation. Together, these results show that multiple solutions exist for meiotic crossover/segregation problems.

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“…The genetic sex of the gonad in rainbow trout (Oncorhynchus mykiss) does not appear to influence sterility, since XXY animal with ovaries and XXX animal with testis show similar characteristics as their diploid counterparts (Lincoln and Scott 1983;Krisfalusi and Cloud 1999;Devlin and Nagahama 2002). Unusual formations of spermatogenic-like cells smaller than oogonia and morphologically similar to spermatocytes were reported from ovary of relatively old triploid rainbow trout (Carrasco et al 1998;Krisfalusi et al 2000). Not all triploid fish are sterile, although levels of sterility differ between species.…”

Section: Introductionmentioning

confidence: 99%

Studies on sperm of diploid and triploid tench, Tinca tinca (L.)

Linhart¹,

Rodina²,

Flajšhans³

et al. 2005

Aquacult Int

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The tench Tinca tinca is an interesting fish from the viewpoint of polyploidy and related atypical reproduction aspects. Triploid tench were produced artificially. Studies of spermiation as well as of sperm motility and structure were performed on several triploid and diploid males simultaneously with individual experimental crosses with diploid females to define their reproductive capacities. The testes of triploids visually looked less developed in the most of cases with lower sperm production (0.05 cm 3 sperm per male), GSI and weight of testes compared to diploids (0.58 cm 3 sperm per male). Analysis of variance showed significant influence of ploidy level on the percentage of motile spermatozoa. Triploidy did not change percentage of live spermatozoa and velocity of spermatozoa at the first time of sperm movement. The study of sperm structure by scanning electron microscopy revealed that most sperm cells were of normal structure with some anomalies. Sperm heads of triploid and diploid males were mostly round-shaped, 1.86±0.2 and 1.6±0.18 lm in diameter. The midpiece of triploid spermatozoa was slightly narrower than that of diploid ones with typical cylindrical shape. Flow cytometry revealed sperm cells of triploids to be largely aneuploid (1.47 n) with high mosaic DNA, oscillating from haploid DNA content (1.0 n) to diploid DNA content (1.9 n). Experimental crosses between triploid males and diploid females revealed that these males were capable to stimulate effective development with relatively high level of fertilization and hatching rates from 0 to 70%. In conclusion, triploidization does not seem to guarantee sterility of tench.

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Long-term, quantitative analysis of gametogenesis in autotriploid rainbow trout, Oncorhynchus mykiss

Cited by 48 publications

References 21 publications

Sexual plasticity of ovarian germ cells in rainbow trout

Sexual plasticity of ovarian germ cells in rainbow trout

Mlh1 Deficiency in Zebrafish Results in Male Sterility and Aneuploid as Well as Triploid Progeny in Females

Studies on sperm of diploid and triploid tench, Tinca tinca (L.)

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