Long-Term Proliferation of Human Embryonic Stem Cell–Derived Neuroepithelial Cells Using Defined Adherent Culture Conditions

Shin, Soojung; Mitalipova, Maisam; Noggle, Scott; Tibbitts, Deanne; Venable, Alison; Rao, Raj R.; Stice, Steven L.

doi:10.1634/stemcells.2004-0150

Cited by 184 publications

(209 citation statements)

References 42 publications

Supporting

Mentioning

197

Contrasting

Unclassified

Order By: Relevance

“…Some in vitro studies have suggested that stem cells lose expression of stem cell markers as they differentiate. 42,43 In our study, we observed that some markers were expressed focally in tumor sections, but there was no co-expression of markers in the same group of cells. This finding may suggest a sequential loss of marker expression during the maturation process, or that within the same tumor there might be different clones of progenitor cells in different stages of maturation depending on how far through the differentiation process the progenitor had proceeded before the process was interrupted.…”

Section: Discussionmentioning

confidence: 44%

Progenitor stem cell marker expression by pulmonary carcinomas

et al. 2010

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 44%

Progenitor stem cell marker expression by pulmonary carcinomas

et al. 2010

View full text Add to dashboard Cite

“…NPs were derived from WA09 hESC and cryopreserved as described previously [2]. They were subcultured on polyornithine-and laminincoated dishes using neurobasal medium (Gibco, Grand Island, NY, http://www.invitrogen.com) supplemented with 2 mM L-glutamine (Gibco), 1Â penicillin/ streptomycin (Gibco), 1Â B27 (Gibco), 20 ng/ml of FGF2 (Sigma-Aldrich, St Louis, MO, http://www.sigma aldrich.com), and 10 ng/ml LIF (Chemicon-Millipore, Billerica, MA, http://www.millipore.com/).…”

Section: Np Propagation and Derivation Of Neuronsmentioning

confidence: 99%

“…We have previously derived a self-renewing but fate-restricted neural progenitor (NP) cell line from hESC that can generate highly enriched neuronal populations under specific conditions [2,3], thus serving as a potentially unlimited source of human neurons for both basic [4] and applied research [5]. This progenitor line has previously been used to study neurotoxicity, differentiation to specific neuronal phenotypes, drug screening, and transplantation experiments in rodent models of stroke [4,5].…”

Section: Introductionmentioning

confidence: 99%

Neurotrophic Effects of Leukemia Inhibitory Factor on Neural Cells Derived from Human Embryonic Stem Cells

et al. 2012

Self Cite

View full text Add to dashboard Cite

Various growth factor cocktails have been used to proliferate and then differentiate human neural progenitor (NP) cells derived from embryonic stem cells (ESC) for in vitro and in vivo studies. However, the cytokine leukemia inhibitory factor (LIF) has been largely overlooked. Here, we demonstrate that LIF significantly enhanced in vitro survival and promoted differentiation of human ESC-derived NP cells. In NP cells, as well as NP-derived neurons, LIF reduced caspase-mediated apoptosis and reduced both spontaneous and H 2 O 2 -induced reactive oxygen species in culture. In vitro, NP cell proliferation and the yield of differentiated neurons were significantly higher in the presence of LIF. In NP cells, LIF enhanced cMyc phosphorylation, commonly associated with self-renewal/proliferation. Also, in differentiating NP cells LIF activated the phosphoinositide 3-kinase and signal transducer and activator of transcription 3 pathways, associated with cell survival and reduced apoptosis. When differentiated in LIF 1 media, neurite outgrowth and ERK1/2 phosphorylation were potentiated together with increased expression of gp130, a component of the LIF receptor complex. NP cells, pretreated in vitro with LIF, were effective in reducing infarct volume in a model of focal ischemic stroke but LIF did not lead to significantly improved initial NP cell survival over nontreated NP cells. Our results show that LIF signaling significantly promotes human NP cell proliferation, survival, and differentiation in vitro. Activated LIF signaling should be considered in cell culture expansion systems for future human NP cell-based therapeutic transplant studies.

show abstract

“…Several methods to obtain neural differentiation have been reported. These include embryoid body formation and selection in NSC medium [5][6][7], sorting with CD133 after differentiation [8], selecting neurosphere forming cells after plating cells in low density [9], a direct differentiation of adherent culture in defined medium [10,11], or enhancing/biasing neural differentiation by altering bone morphogenic protein/transforming growth factor-␤ signaling or altering Notch signaling [12][13][14]. In all of these processes, a number of nestin-positive dividing populations can be obtained that express neural markers, and these populations can be induced to differentiate into neurons, astrocytes, and immature oligodendrocytes.…”

Section: Introductionmentioning

confidence: 99%

Whole Genome Analysis of Human Neural Stem Cells Derived from Embryonic Stem Cells and Stem and Progenitor Cells Isolated from Fetal Tissue

Shin¹,

Sun

Liu³

et al. 2007

Stem Cells

Self Cite

View full text Add to dashboard Cite

Multipotent neural stem cells (NSC) have been derived from human embryonic stem cells (hESC) as well as isolated from fetal tissues. However, there have been few exclusive markers of NSC identified to date, and the differences between NSC from various sources are poorly understood. Although cells isolated from these two sources share many important characteristics, it is not clear how closely they are related in terms of gene expression. Here, we compare the gene expression profiles of 11 lines of NSC derived from hESC (ES_NSC), four lines of NSC isolated from fetus (F_NSC), and two lines of restricted progenitors in order to characterize these cell populations and identify differences between NSC derived from these two sources. We showed that ES_NSC were clustered together with high transcriptional similarities but were distinguished from F_NSC, oligodendrocyte precursor cells, and astrocyte precursor cells. There were 17 genes expressed in both ES_NSC and F_NSC whose expression was not identified in restricted neural progenitors. Furthermore, the major differences between ES_NSC and F_NSC were mostly observed in genes related to the key neural differentiation pathways. Here, we show that comparison of global gene expression profiles of ES_NSC, F_NSC, and restricted neural progenitor cells makes it possible to identify some of the common characteristics of NSC and differences between similar stem cell populations derived from hESCs or isolated from fetal tissue.

show abstract

Long-Term Proliferation of Human Embryonic Stem Cell–Derived Neuroepithelial Cells Using Defined Adherent Culture Conditions

Cited by 184 publications

References 42 publications

Progenitor stem cell marker expression by pulmonary carcinomas

Progenitor stem cell marker expression by pulmonary carcinomas

Neurotrophic Effects of Leukemia Inhibitory Factor on Neural Cells Derived from Human Embryonic Stem Cells

Whole Genome Analysis of Human Neural Stem Cells Derived from Embryonic Stem Cells and Stem and Progenitor Cells Isolated from Fetal Tissue

Contact Info

Product

Resources

About