Long-term potentiation is associated with increased [3H]AMPA binding in rat hippocampus

Tocco, Georges; Maren, Stephen; Shors, Tracey J.; Baudry, Michel; Thompson, Richard F.

doi:10.1016/0006-8993(92)90767-4

Cited by 103 publications

(59 citation statements)

References 31 publications

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“…Electrophysiological studies found a specific increase in ␣-amino-2-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor-mediated current following LTP, [24][25][26] which supported an increase in AMPA receptor function as the mechanism of LTP expression. This was further supported by Tocco et al, 27 who found enhanced binding of [ 3 H] AMPA following high-frequency stimulation of the perforant path.…”

Section: Molecular Mechanisms Underlying the Ecs Effectsupporting

confidence: 60%

“…Shors et al 63 suggested that stress induced the same cellular processes as high-frequency stimulation for two main reasons: (1) stress or prior LTP induction produced a similar decrease in experimental LTP 2 h later; 63 and (2) both stress 70 and LTP induction 27 increased AMPA binding throughout the hippocampus. However, there were no significant increases in basal synaptic responses in stressed rats compared with controls.…”

Section: Molecular Mechanisms Underlying the Stress Effectsmentioning

confidence: 99%

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Antidepressant mechanisms: functional and molecular correlates of excitatory amino acid neurotransmission

Stewart

Reid

2002

Mol Psychiatry

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Specific targeting of the serotonergic and noradrenergic systems for the development of antidepressant compounds has resulted in drugs with more favourable side-effect profiles but essentially no greater efficacy than those compounds discovered more than 40 years ago. Alternative targets are now being considered in the hope that they will have a faster onset of action and be useful for those patients currently unresponsive to conventional treatments. Excitatory amino acid neurotransmission has been attributed various roles in both normal and abnormal brain function. The N-methyl-D-aspartate receptor in particular has long been postulated to play a role in the formation of memories. Major depressive disorder is characterised by alterations in cognitive function, as well as affect. Although there is evidence that early adverse events and stress can have a causal influence on depression, the underlying neurobiology of the disorder is poorly understood. This review will document current evidence for the involvement of excitatory amino acid neurotransmission in the pathophysiology of the affective disorders. The preclinical literature suggests that both electroconvulsive stimulation and antidepressant drugs can affect hippocampal long-term potentiation and the expression of excitatory amino acid receptor subtypes. Exposing animals to stress, including the kind that produces learned helplessness, can also affect synaptic plasticity in the hippocampus. There is clinical evidence that patients with chronic depression have structural brain abnormalities, including hippocampal atrophy, and a preliminary study has shown that an N-methyl-D-aspartate receptor antagonist may have antidepressant efficacy. Molecular Psychiatry (2002) 7, S15-S22.

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Section: Molecular Mechanisms Underlying the Ecs Effectsupporting

confidence: 60%

Section: Molecular Mechanisms Underlying the Stress Effectsmentioning

confidence: 99%

Antidepressant mechanisms: functional and molecular correlates of excitatory amino acid neurotransmission

Stewart

Reid

2002

Mol Psychiatry

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“…Tests for a change in receptor affinity proved negative (31), pointing to a conductance Increase as the agent of LTP expression. However, another group using a different autoradiographic technique has reported that LTP increased binding to the AMPA receptor (43). The discrepancies between their results and ours need to be examined but i. is likely that LTP -expression Is due to a change in receptor conformation that affects ligand affinity, channel conductance, and channel kinetics.…”

Section: (J{4~3contrasting

confidence: 59%

Synaptic Plasticity and Memory Formation.

Lynch¹

1992

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“…These groups allowed us to examine the effects of both the quantity and pattern of HFS on hippocampal LTP and glutamate receptor binding. Data from the HF-200 animals were as reported (10). Current intensities were adjusted to elicit a 1-to 2-mV population spike (PS) in all subjects.…”

Section: Methodsmentioning

confidence: 99%

Postsynaptic factors in the expression of long-term potentiation (LTP): increased glutamate receptor binding following LTP induction in vivo.

Maren

Tocco

Standley

et al. 1993

Proc. Natl. Acad. Sci. U.S.A.

120

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Several lines of evidence indicate that LTP in the hippocampus is associated with a change in the properties of postsynaptic glutamate receptors. In the present study, we used quantitative autoradiography to examine the binding properties of the a-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) and N-methyl-D-aspartate subclasses of glutamate receptors in frozen brain sections obtained from rats in which perforant-path LTP LTP of excitatory synaptic transmission in the hippocampus is an enduring form of synaptic enhancement that may underlie some forms of mammalian learning and memory (1, 2). While consensus has been reached on the cellular mechanisms involved in triggering LTP, the nature of the persistent synaptic modification underlying the expression of LTP is still a matter of debate (3). There is a growing body of evidence that LTP is expressed, at least in part, by a persistent modification of postsynaptic a-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptors, a subclass of ionotropic glutamate receptors (4-9). This modification may be variously expressed as an increase in the binding affinity of AMPA receptors, an increase in the number of AMPA binding sites, or a change in the kinetics of AMPA receptor-gated ion channels. To further restrict these possibilities, we have examined the binding properties of postsynaptic AMPA and N-methyl-D-aspartate (NMDA) receptors (another subclass ofionotropic glutamate receptors) in frozen brain sections following perforant-path LTP induction in vivo. We report a selective increase in hippocampal AMPA receptor binding following perforant-path LTP induction that is apparently mediated by an increase in the number of AMPA binding sites. These results suggest that a change in the binding properties of AMPA receptors contributes to the enhanced synaptic transmission observed at hippocampal synapses following LTP induction.The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact. MATERIALS AND METHODSSubjects and Surgery. Adult male Long-Evans rats (250-300 g) were anesthetized with an i.p. injection of sodium pentobarbital (65 mg/kg) and implanted with a recording electrode in the hilus of the dentate gyrus and a bipolar stimulating electrode in the perforant path (9). The electrodes consisted of Epoxylite-coated stainless-steel pins with the recording and stimulating surfaces formed by removing the insulation at the tips. The electrode tip lengths were 50 and 500 ,um for recording and stimulating electrodes, respectively.Acute Electrophysiology. All electrophysiological testing was performed under surgical anesthesia. Extracellular field potentials evoked by single-pulse perforant-path stimulation pulses (0.1 msec, 0.05 Hz) were recorded in the dentate gyrus during a 10-min interval before and a 60-min interval following high-or low-frequency perforant-path stimulation (HFS or LFS). Fig. 1A repr...

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Long-term potentiation is associated with increased [3H]AMPA binding in rat hippocampus

Cited by 103 publications

References 31 publications

Antidepressant mechanisms: functional and molecular correlates of excitatory amino acid neurotransmission

Antidepressant mechanisms: functional and molecular correlates of excitatory amino acid neurotransmission

Synaptic Plasticity and Memory Formation.

Postsynaptic factors in the expression of long-term potentiation (LTP): increased glutamate receptor binding following LTP induction in vivo.

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