Long-term ovarian function in sheep after ovariectomy and autotransplantation of cryopreserved cortical strips

Baird, D. T.; Campbell, Bruce; Souza, Camilla Mariane Menezes; Telfer, Evelyn E.

doi:10.1016/j.ejogrb.2003.11.023

Cited by 70 publications

(47 citation statements)

References 20 publications

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“…Similar increases in follicular growth after cryopreservation and transplantation of ovine cortical tissue were observed [26]. Therefore, it is hypothesized that the ovary under physiological conditions is dominated by inhibitory mechanisms, which are no longer available during transplantation [14,26]. Our results also showed that VOAT mice had higher PCNA expression than intact mice.…”

Section: Discussionsupporting

confidence: 75%

“…It has been reported that PCNA-positive follicles after cryopreservation and 7-day autotransplantation were significantly increased, including the antral stage, in the ovarian tissues compared with control tissue [14]. Similar increases in follicular growth after cryopreservation and transplantation of ovine cortical tissue were observed [26]. Therefore, it is hypothesized that the ovary under physiological conditions is dominated by inhibitory mechanisms, which are no longer available during transplantation [14,26].…”

Section: Discussionsupporting

confidence: 51%

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Vitrified‐warmed ovarian tissue autotransplantation into ovariectomized mice restores sufficient ovarian function to support full‐term pregnancy

Matsumoto

Ezoe

Mitsui

et al. 2011

Reprod Medicine & Biology

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Purpose Our previous study demonstrated that heterotopic autotransplantation of fresh ovarian tissue followed by transfer of blastocysts supported full-term pregnancy in the mouse. In the present study, to address whether vitrified-warmed ovarian tissue has the potential to support uterine preparation for implantation and subsequent pregnancy to full term, we examined vitrified-warmed ovarian tissue autotransplantation (VOAT) in mice. Methods VOAT into kidney capsules was performed for sexual cycle-ceased mice after 7 days of ovariectomy. Uterine potential of decidualization was examined by oil infusion on day 4 of pseudopregnancy. Immunohistochemical analysis was performed to examine the potential in VOAT ovarian tissues. Blastocysts were transferred into uteri on day 4 of pseudopregnancy. Results In VOAT mice, uterine decidualization on day 8 of pseudopregnancy was the same as that in intact mice. Blastocyst transfer into the pseudopregnant VOAT mice showed the same rates of pregnancy and live birth pups as intact mice, while less steroidogenesis in the corpus luteum was detected in VOAT mice. Conclusions The autotransplantation of vitrified-warmed ovarian tissues after 7 days of ovariectomy restored their sexual cycle and then supported their pregnancy and production of offspring.

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Section: Discussionsupporting

confidence: 75%

Section: Discussionsupporting

confidence: 51%

Vitrified‐warmed ovarian tissue autotransplantation into ovariectomized mice restores sufficient ovarian function to support full‐term pregnancy

Matsumoto

Ezoe

Mitsui

et al. 2011

Reprod Medicine & Biology

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“…The secretion of inhibin A in ovarian grafts is decreased which in turn causes a rise in FSH concentrations and may result from the reduced number of preantral follicles in ovarian grafts. LH concentrations are also elevated in sheep with ovarian grafts, however progesterone and oestrogen levels are normal throughout the cycle (Baird et al 2004). Whereas in rats, oestrogen and cyclic activity are altered in heterotopic ovarian grafts (Petroianu et al 2005).…”

Section: Discussionmentioning

confidence: 84%

“…Abnormalities in hormonal concentrations may alter follicle development. There is evidence that in the grafts in mice and sheep, both the rate of recruitment and the rate of follicle development are accelerated following grafting , Baird et al 2004). This indicates that abnormalities in ovarian function within ovarian grafts may be due to an altered endocrine environment within ovarian graft recipients.…”

Section: Discussionmentioning

confidence: 99%

Graft site and gonadotrophin stimulation influences the number and quality of oocytes from murine ovarian tissue grafts

Yang¹,

Cox²,

Jenkin³

et al. 2006

Reproduction

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Ovarian tissue cryopreservation and subsequent transplantation can restore fertility in cancer patients. This study used a mouse ovarian grafting model to investigate whether the graft site (bursal cavity, the kidney capsule or subcutaneous) influences the number, fertilization rate and developmental potential of oocytes recovered from grafts and whether using a standard gonadotrophin stimulation protocol would increase oocyte yield from the grafts. Mouse ovarian tissue was grafted into four week old mice and collected three weeks later. Graft recipients were treated either with or without exogenous gonadotrophin stimulation prior to graft collection. Grafted ovaries yielded oocytes that were either at the germinal vesicle (GV) stage or mature metaphase II (MII) stage at collection. These GV oocytes were matured before in vitro fertilization (IVF), while the MII oocytes underwent IVF immediately. Oocytes collected from the oviducts of non-grafted superovulated mice of the same age served as controls. Two-cell embryos were transferred to pseudopregnant recipients and recovered at day 15 of gestation or left to go to term. Graft retrieval and the number of oocytes from each graft were lowest from the subcutaneous graft site. The number of two-cell embryos produced was significantly higher for oocytes from the grafts to the bursa as compared with the other sites. All graft sites gave rise to embryos with comparable implantation rates and developmental potential to fetuses and offspring following transfer. However, the oocytes from grafted ovaries had a significantly lower developmental potential when compared with the control group. Stimulation with exogenous gonadotrophins did not significantly increase oocyte yield from grafted ovaries but did enhance oocyte maturation and development. In conclusion, graft site affects the number and quality of oocytes produced from ovarian grafts.

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“…A number of scholars have proposed that an osteoporosis model can be established within 6 months through the use of various testing methods, such as biomechanical testing, bone histophotometry analysis or DXA (18,(26)(27)(28); however, there are limitations, including a small number of samples and single specimen testing methods. Recent research has demonstrated that the BMD of the vertebral body in ovariectomized sheep exhibits a significant downward trend after 1 year; however, no statistically significant difference was identified when compared with a sham-control group (18).…”

Section: Discussionmentioning

confidence: 99%

Long-term effects of ovariectomy on the properties of bone in goats

Wang

Tang

et al. 2015

Experimental and Therapeutic Medicine

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Abstract. Large animal models of osteoporosis are essential for osteoporosis research. However, the time required to establish an accurate osteoporosis model is unknown. Therefore, the aim of the present study was to establish a large animal model of osteoporosis in goats. In total, 14 Chinese goats were divided into an ovariectomized (OVX, n=7) or sham-operated (SHAM, n=7) group. Vertebral bodies were used to measure the bone mineral density (BMD) prior to the ovariectomy and at 24 months after the ovariectomy. In addition, the BMD of the femoral neck, femoral diaphysis and tibial diaphysis were measured 24 months postoperatively. Bone samples from the vertebral body, femoral head and femoral neck were scanned by micro-computed tomography (CT) to visualize the trabecular and cortical microstructure. Furthermore, the vertebral body, femoral head, femoral neck and tibial diaphysis were analyzed for mechanical strength. The BMD of vertebral body of the OVX group decreased significantly (P<0.01) at 24 months after the ovariectomy when compared with the baseline measurements. Micro-CT scans of the vertebral body revealed that the bone volume fraction, trabecular number, trabecular thickness and the degree of anisotropy decreased by 37.1, 36.7, 10.5 and 16.5%, respectively (P<0.01) in the OVX group when compared with the SHAM group. Additionally, the specific bone surface and trabecular spacing significantly increased by 37.7 and 62%, respectively in the OVX group (P<0.001). Cortical bone porosity in the vertebral body and femoral neck was greater in the OVX group when compared with the SHAM group (P<0.05). In addition, mechanical testing revealed a statistically significant difference between the vertebral bodies of the OVX group and the SHAM group. In conclusion, the present study demonstrated that an ovariectomy was able to induce significant osteoporosis and deterioration of mechanical properties in the bones of goats.

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Long-term ovarian function in sheep after ovariectomy and autotransplantation of cryopreserved cortical strips

Cited by 70 publications

References 20 publications

Vitrified‐warmed ovarian tissue autotransplantation into ovariectomized mice restores sufficient ovarian function to support full‐term pregnancy

Vitrified‐warmed ovarian tissue autotransplantation into ovariectomized mice restores sufficient ovarian function to support full‐term pregnancy

Graft site and gonadotrophin stimulation influences the number and quality of oocytes from murine ovarian tissue grafts

Long-term effects of ovariectomy on the properties of bone in goats

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