Long-term outcomes of patch tracheoplasty using collagenous tissue membranes (biosheets) produced by in-body tissue architecture in a beagle model

Umeda, Satoshi; Nakayama, Yasuhide; Terazawa, Takeshi; Iwai, Ryosuke; Hiwatashi, Shohei; Nakahata, Kengo; Takama, Yuichi; Okuyama, Hiroomi

doi:10.1007/s00595-019-01818-5

Cited by 9 publications

(7 citation statements)

References 17 publications

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“…In animal experiments, iBTA has been used to make heart valves that can maintain function in goats for 1 year 28 . In addition, examples of application in digestive organs such as esophagus, 29 respiratory organs such as trachea, 30 urinary organs such as bladder, 31 and plastic organs such as peritoneum 32 have also been reported.…”

Section: Discussionmentioning

confidence: 99%

Pre‐implantation evaluation of a small‐diameter, long vascular graft (Biotube®) for below‐knee bypass surgery in goats

et al. 2022

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There are no small-diameter, long artificial vascular grafts for below-knee bypass surgery in chronic limb-threatening ischemia. We have developed tissue-engineered vascular grafts called "Biotubes ® " using a completely autologous approach called in-body tissue architecture (iBTA). This study aimed at pre-implantation evaluation of Biotube and its in vivo preparation device, Biotube Maker, for use in below-knee bypass surgery. Forty nine makers were subcutaneously embedded into 17 goats for predetermined periods (1, 2, or 3 months). All makers produced Biotubes as designed without inflammation over all periods, with the exception of a few cases with minor defects (success rate: 94%). Small hole formation occurred in only a few cases. All Biotubes obtained had an inner diameter of 4 mm and a length of 51 to 52 cm with a wall thickness of 594 ± 97 μm. All Biotubes did not kink when completely bent under an internal pressure of 100 mmHg and did not leak without any deformation under a water pressure of 200 mmHg. Their burst strength was 2409 ± 473 mmHg, and suture retention strength was 1.75 ± 0.27 N, regardless of the embedding period, whereas tensile strength increased from 7.5 ± 1.3 N at 1 month to 9.7 ± 2.0 N at 3 months with the embedding period. The amount of water leakage from the needle holes prepared in the Biotube wall was approximately 1/7th of that in expanded polytetrafluoroethylene vascular grafts. The Biotubes could be easily connected to each other without cutting or anastomosis leaks. They could be stored for at least 1 year at room temperature. This study confirmed that even Biotubes formed 1 month after embedding of Biotube Makers had properties comparable to arteries.biotube, chronic limb-threatening ischemia, in body tissue architecture, small diameter vascular graft, tissue engineering | INTRODUCTIONPatients with chronic limb-threatening ischemia (CLTI) present with resting pain, foot ulcer, or gangrene. Due to the severity of the disease, hundreds of thousands of patients worldwide undergo lower limb amputations each year. 1 The prognosis after amputation is extremely poor, with a mortality rate of 30-50% and a contralateral leg amputation rate of 25% within 1 year, which is a life-threatening

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Section: Discussionmentioning

confidence: 99%

Pre‐implantation evaluation of a small‐diameter, long vascular graft (Biotube®) for below‐knee bypass surgery in goats

et al. 2022

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“…Heatmap representing the proportion of publications by year that utilized specific translational research methodologies from construct characterization to human trial to investigate laryngeal tissue engineering 85‐87,91,92,94,96,97,198‐306 …”

Section: Discussionmentioning

confidence: 99%

Tissue engineering applications in otolaryngology—The state of translation

Niermeyer

Rodman

et al. 2020

Laryngoscope Investig Oto

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While tissue engineering holds significant potential to address current limitations in reconstructive surgery of the head and neck, few constructs have made their way into routine clinical use. In this review, we aim to appraise the state of head and neck tissue engineering over the past five years, with a specific focus on otologic, nasal, craniofacial bone, and laryngotracheal applications. A comprehensive scoping search of the PubMed database was performed and over 2000 article hits were returned with 290 articles included in the final review. These publications have addressed the hallmark characteristics of tissue engineering (cellular source, scaffold, and growth signaling) for head and neck anatomical sites. While there have been promising reports of effective tissue engineered interventions in small groups of human patients, the majority of research remains constrained to in vitro and in vivo studies aimed at furthering the understanding of the biological processes involved in tissue engineering. Further, differences in functional and cosmetic properties of the ear, nose, airway, and craniofacial bone affect the emphasis of investigation at each site. While otolaryngologists currently play a role in tissue engineering translational research, continued multidisciplinary efforts will likely be required to push the state of translation towards tissue‐engineered constructs available for routine clinical use. Level of Evidence NA.

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“…The Biotube has already been clinically applied as an alternative blood vessel for bypass to a chronic limb-threating ischemia patient 13 or hemodialysis patients. The patch implantation of the Biosheets into the partial tracheal defects in beagle dogs resulted in the reconstruction of the tracheal tissue by covering the ciliated epithelium on the lumen surface and the partial infiltration of chondrocyte aggregates in the middle wall 18 . When the Biotube was implanted circumferentially into the trachea, stenosis occurred within a few days owing to its insufficient strength.…”

Section: Introductionmentioning

confidence: 99%

Successful tracheal regeneration using biofabricated autologous analogues without artificial supports

Hiwatashi

Iwai

Nakayama³

et al. 2022

Sci Rep

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Tracheas have a tubular structure consisting of cartilage rings continuously joined by a connective tissue membrane comprising a capillary network for tissue survival. Several tissue engineering efforts have been devoted to the design of scaffolds to produce complex structures. In this study, we successfully fabricated an artificial materials-free autologous tracheal analogue with engraftment ability by combining in vitro cell self-aggregation technique and in-body tissue architecture. The cartilage rings prepared by aggregating chondrocytes on designated culture grooves that induce cell self-aggregation were alternately connected to the connective tissues to form tubular tracheal analogues by subcutaneous embedding as in-body tissue architecture. The tracheal analogues allogeneically implanted into the rat trachea matured into native-like tracheal tissue by covering of luminal surfaces by the ciliated epithelium with mucus-producing goblet cells within eight months after implantation, while maintaining their structural integrity. Such autologous tracheal analogues would provide a foundation for further clinical research on the application of tissue-engineered tracheas to ensure their long-term functionality.

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Long-term outcomes of patch tracheoplasty using collagenous tissue membranes (biosheets) produced by in-body tissue architecture in a beagle model

Cited by 9 publications

References 17 publications

Pre‐implantation evaluation of a small‐diameter, long vascular graft (Biotube®) for below‐knee bypass surgery in goats

Pre‐implantation evaluation of a small‐diameter, long vascular graft (Biotube®) for below‐knee bypass surgery in goats

Tissue engineering applications in otolaryngology—The state of translation

Successful tracheal regeneration using biofabricated autologous analogues without artificial supports

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