Long-term observation of airway reconstruction using decellularized tracheal allografts in micro-miniature pigs at growing stage

Ohno, Michinobu; Fuchimoto, Yasushi; Higuchi, Masataka; Yamaoka, Tetsuji; Komura, Makoto; Umezawa, Akihiro; Hsu, Huai Che; Enosawa, Shin; Kuroda, Takeshi

doi:10.1016/j.reth.2020.04.010

Cited by 7 publications

(5 citation statements)

References 23 publications

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“…It has been confirmed that a natural decellularized tracheal extracellular matrix (dtECM) that meets the above requirements can be effectively obtained using decellularization 20,35,36 . It has been demonstrated that dtECM supports neo‐epithelialization, endothelialization, and chondrocyte viability, 40 and it may serve as a promising biomaterial for tracheal reconstitution 41 . Although natural dtECM meets the requirements of most idealized tracheal grafts, the critical limitation is that the biomechanical properties of the scaffold are considerably reduced, resulting in serious complications, such as the softening and stenosis of the grafts in the early stage after orthotopic transplantation 14,42 .…”

Section: Discussionmentioning

confidence: 89%

“… 20 , 35 , 36 It has been demonstrated that dtECM supports neo‐epithelialization, endothelialization, and chondrocyte viability, 40 and it may serve as a promising biomaterial for tracheal reconstitution. 41 Although natural dtECM meets the requirements of most idealized tracheal grafts, the critical limitation is that the biomechanical properties of the scaffold are considerably reduced, resulting in serious complications, such as the softening and stenosis of the grafts in the early stage after orthotopic transplantation. 14 , 42 Pure synthetic tracheal grafts have favorable biomechanical properties; however, they exhibit numerous graft‐related complications, including obstructive granulation tissue and anastomotic leaks, lack of vascularization, epithelial lining, or fusion into surrounding tissues.…”

Section: Discussionmentioning

confidence: 99%

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Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one‐step

Sun

Shen

Zhang

et al. 2023

Bioengineering & Transla Med

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Formation of functional and perfusable vascular network is critical to ensure the long-term survival and functionality of the engineered tissue tracheae after transplantation. However, the greatest challenge in tracheal-replacement therapy is the promotion of tissue regeneration by rapid graft vascularization. Traditional prevascularization methods for tracheal grafts typically utilize omentum or muscle flap wrapping, which requires a second operation; vascularized segment tracheal orthotopic transplantation in one step remains difficult. This study proposes a method to construct a tissue-engineered tracheal graft, which directly forms the microvascular network after orthotopic transplantation in vivo. The focus of this study was the preparation of a hybrid tracheal graft that is non-immunogenic, has good biomechanical properties, supports cell proliferation, and quickly vascularizes. The results showed that vacuum-assisted decellularized trachea-polycaprolactone hybrid scaffold could match most of the above requirements as closely as possible. Furthermore, endothelial progenitor cells (EPCs) were extracted and used as vascularized seed cells and seeded on the surfaces of hybrid grafts before and during the tracheal orthotopic transplantation. The results showed that the microvascularized tracheal grafts formed maintained the survival of the recipient, showing a satisfactory therapeutic outcome. This is the first study to utilize EPCs for microvascular construction of long-segment trachea in one-step; the approach represents a promising method for microvascular tracheal reconstruction.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one‐step

Sun

Shen

Zhang

et al. 2023

Bioengineering & Transla Med

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“…Porcine trachea was obtained from Tokyo Shibaura Zoki KK (Tokyo, Japan) and decellularized using HHP, as reported previously [ 25 , 26 ]. Tracheal tissues were cut into 2 × 2 cm 2 and packed in plastic bags with 1% penicillin-streptomycin (100 U/mL penicillin and 100 μg/mL streptomycin; Sigma-Aldrich, St. Louis, MO) saline solution, sealed using a heat sealer.…”

Section: Methodsmentioning

confidence: 99%

High pressure pasteurization: Simultaneous native tissue decellularization and sterilization

Otaka,

Yamamoto,

Yamaoka

2024

Regenerative Therapy

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“…A suitable tracheal substitute is required for successful airway reconstruction. Many ways to develop acceptable tracheal substitutes have been tested in clinical practice over the years, including autologous tracheal transplantation [ 9 , 10 , 11 ], tracheal allotransplantation [ 12 , 13 , 14 , 15 , 16 , 17 , 18 ], and stent implantation [ 19 ], as shown in Figure 1 . Autologous transplantation is regarded as a tracheal transplantation procedure that causes the least amount of immune rejection and can prevent some potential issues, like infection and cracking [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

A Review of Woven Tracheal Stents: Materials, Structures, and Application

Chen

Huang

et al. 2022

JFB

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The repair and reconstruction of tracheal defects is a challenging clinical problem. Due to the wide choice of materials and structures, weaving technology has shown unique advantages in simulating the multilayer structure of the trachea and providing reliable performance. Currently, most woven stent-based stents focus only on the effect of materials on stent performance while ignoring the direct effect of woven process parameters on stent performance, and the advantages of weaving technology in tissue regeneration have not been fully exploited. Therefore, this review will introduce the effects of stent materials and fabric construction on the performance of tracheal stents, focusing on the effects of weaving process parameters on stent performance. We will summarize the problems faced by woven stents and possible directions of development in the hope of broadening the technical field of artificial trachea preparation.

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Long-term observation of airway reconstruction using decellularized tracheal allografts in micro-miniature pigs at growing stage

Cited by 7 publications

References 23 publications

Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one‐step

Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one‐step

High pressure pasteurization: Simultaneous native tissue decellularization and sterilization

A Review of Woven Tracheal Stents: Materials, Structures, and Application

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