Long-term moderate exercise enhances specific proteins that constitute neurotrophin signaling pathway: A TMT-based quantitative proteomic analysis of rat plasma

Wei, Yuan; Fang, Chunlu; Liu, Shujing; Yang, Wenqi; Wei, Li-Shen; Lei, Xiao-Juan; Hu, Fei; Huang, Hao-Ying; Li, Wei; Chen, Wei; Li, Liangming; Long, Yue-Sheng

doi:10.1016/j.jprot.2018.06.017

Cited by 20 publications

(14 citation statements)

References 64 publications

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“…Physical exercise results in increased production of neurotrophic factors and regulation of levels of some cytokines, cytokine inhibitors and chemokines, all of which may contribute to improving motor function and cognition in neurodegenerative disorders (Svensson et al, 2015; Wei et al, 2018). In SCI, local levels of cytokines critically influence the kinetics of neuron survival, axon degeneration and regeneration (Sun et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Wheel Running Improves Motor Function and Spinal Cord Plasticity in Mice With Genetic Absence of the Corticospinal Tract

Zhang

Yang

Weng

et al. 2019

Front. Cell. Neurosci.

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Our previous studies showed that mutant mice with congenital absence of the corticospinal tract (CST) undergo spontaneous remodeling of motor networks to partially compensate for absent CST function. Here, we asked whether voluntary wheel running could further improve locomotor plasticity in CST-deficient mice. Adult mutant mice were randomly allocated to a “runners” group with free access to a wheel, or a “non-runners” group with no access to a wheel. In comparison with non-runners, there was a significant motor improvement including fine movement, grip strength, decreased footslip errors in runners after 8-week training, which was supported by the elevated amplitude of electromyography recording and increased neuromuscular junctions in the biceps. In runners, terminal ramifications of monoaminergic and rubrospinal descending axons were significantly increased in spinal segments after 12 weeks of exercise compared to non-runners. 5-ethynyl-2′-deoxyuridine (EDU) labeling showed that proliferating cells, 90% of which were Olig2-positive oligodendrocyte progenitors, were 4.8-fold more abundant in runners than in non-runners. In 8-week runners, RNAseq analysis of spinal samples identified 404 genes up-regulated and 398 genes down-regulated, and 69 differently expressed genes involved in signal transduction, among which the NF-κB, PI3K-Akt and cyclic AMP (cAMP) signaling were three top pathways. Twelve-week training induced a significant elevation of postsynaptic density protein 95 (PSD95), synaptophysin 38 and myelin basic protein (MBP), but not of brain derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF) and insulin like growth factor-1 (IGF-1). Thus, locomotor training activates multiple signaling pathways, contributes to neural plasticity and functional improvement, and might palliate locomotor deficits in patients.

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Section: Discussionmentioning

confidence: 99%

Wheel Running Improves Motor Function and Spinal Cord Plasticity in Mice With Genetic Absence of the Corticospinal Tract

Zhang

Yang

Weng

et al. 2019

Front. Cell. Neurosci.

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“…According to the manufacturer's instructions, 100 μg peptide mixture of each sample was labeled using tandem mass tags (TMT) isobaric label reagents (Thermo Fisher Scientific). Subsequently, pierce high pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific, Waltham, MA, United States) was used to fractionate TMT-labeled digest samples into 15 fractions by an increasing acetonitrile stepgradient elution according to instructions (Wei et al, 2018). The fractions were dried, lyophilized, and stored at -80 C until LC-MS/MS analysis.…”

Section: Tmt Labeling and Peptide Fractionationmentioning

confidence: 99%

Galectin-3 Mediated Inflammatory Response Contributes to Neurological Recovery by QiShenYiQi in Subacute Stroke Model

Wang

et al. 2021

Front. Pharmacol.

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Effective therapies for stroke are still limited due to its complex pathological manifestations. QiShenYiQi (QSYQ), a component-based Chinese medicine capable of reducing organ injury caused by ischemia/reperfusion, may offer an alternative option for stroke treatment and post-stroke recovery. Recently, we reported a beneficial effect of QSYQ for acute stroke via modulation of the neuroinflammatory response. However, if QSYQ plays a role in subacute stroke remains unknown. The pharmacological action of QSYQ was investigated in experimental stroke rats which underwent 90 min ischemia and 8 days reperfusion in this study. Neurological and locomotive deficits, cerebral infarction, brain edema, and BBB integrity were assessed. TMT-based quantitative proteomics were performed to identify differentially expressed proteins following QSYQ treatment. Immunohistochemistry, western blot analysis, RT-qPCR, and ELISA were used to validate the proteomics data and to reveal the action mechanisms. Therapeutically, treatment with QSYQ (600 mg/kg) for 7 days significantly improved neurological recovery, attenuated infarct volume and brain edema, and alleviated BBB breakdown in the stroke rats. Bioinformatics analysis indicated that protein galectin-3 and its mediated inflammatory response was closely related to the beneficial effect of QSYQ. Specially, QSYQ (600 mg/kg) markedly downregulated the mRNA and protein expression levels of galectin-3, TNF-α, and IL-6 in CI/RI brain as well as serum levels of TNF-α and IL-6. Overall, our findings showed that the effective action of QSYQ against the subacute phase of CI/RI occurs partly via regulating galectin-3 mediated inflammatory reaction.

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“…Protein extraction and peptide digestion were performed as described previously [18]. Briefly, lung tissues were lysed in RIPA lysis buffer (Keygen Biotech), and the proteins in lysates were concentrated and digested with Trypsin Gold (Promega) for 16 h at 37°C.…”

Section: Protein Extraction Peptide Digestion and Labelingmentioning

confidence: 99%

“…The samples were dried by vacuum and dissolved in 0.1% (v/v) formic acid. Next the samples were subjected to shotgun proteomics analyses on EASY-nLCTM 1200 UHPLC system (ThermoFisher) coupled to an Orbitrap Q Exactive HF-X mass spectrometer (ThermoFisher) as described previously [18].…”

Section: Lc-ms/ms Analysismentioning

confidence: 99%

Proteomic analysis of sex differences in hyperoxic lung injury in neonatal mice

Cheng¹,

Wang²,

Wu³

et al. 2020

Int. J. Med. Sci.

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Sex-specific differences in the severity of bronchopulmonary dysplasia (BPD) are due to different susceptibility to hyperoxic lung injury, but the mechanism is unclear. In this study, neonatal male and female mouse pups (C57BL/6J) were exposed to hyperoxia and lung tissues were excised on postnatal day 7 for histological analysis and tandem mass tags proteomic analysis. We found that the lung sections from the male mice following postnatal hyperoxia exposure had increased alveolar simplification, significant aberrant pulmonary vascularization and arrest in angiogenesis compared with females. Comparison of differentially expressed proteins revealed 377 proteins unique to female and 425 unique to male as well as 750 proteins in both male and female. Bioinformatics analysis suggested that several differentially expressed proteins could contribute to the differences in sex-specific susceptibility to hyperoxic lung injury. Our results may help identify sex-specific biomarkers and therapeutic targets of BPD.

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Long-term moderate exercise enhances specific proteins that constitute neurotrophin signaling pathway: A TMT-based quantitative proteomic analysis of rat plasma

Cited by 20 publications

References 64 publications

Wheel Running Improves Motor Function and Spinal Cord Plasticity in Mice With Genetic Absence of the Corticospinal Tract

Wheel Running Improves Motor Function and Spinal Cord Plasticity in Mice With Genetic Absence of the Corticospinal Tract

Galectin-3 Mediated Inflammatory Response Contributes to Neurological Recovery by QiShenYiQi in Subacute Stroke Model

Proteomic analysis of sex differences in hyperoxic lung injury in neonatal mice

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