Long-term in vivo biodistribution imaging and toxicity of polyacrylic acid-coated upconversion nanophosphors

Xiong, Liqin; Yang, Tianshe; Yang, Yang; Xu, Congjian; Li, Fuyou

doi:10.1016/j.biomaterials.2010.05.065

Cited by 516 publications

(425 citation statements)

References 37 publications

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“…As noted above, the narrow line luminescence, long ES lifetimes and photostability of Ln III -doped UCNPs make these particularly well suited for biological imaging. In this context, there have been some really exciting developments in cellular imaging [103][104][105][106][107] and in vivo imaging with small animals [103,104,[108][109][110][111]. For example, strongly fluorescent core : shell β-NaYF 4 :Yb,Er UCNPs, coated with a thin layer of SiO 2 and modified with amino groups, were covalently linked to rabbit anti-CEA8 antibodies.…”

Section: Uncaging Using Upconversion Nanoparticles (A) Introduction Tmentioning

confidence: 99%

“…No apparent cytotoxicity was found. Subsequent long-term studies by Xiong et al [111] of whole body imaging with polyacrylic acid-coated NaYF 4 :Yb/Tm UCNPs were able to track the biodistribution and excretion of the rare earth phosphors in mice. This study showed that injections of 15 mg kg −1 of these UCNPs led to no adverse effects on the health of the mice.…”

Section: Uncaging Using Upconversion Nanoparticles (A) Introduction Tmentioning

confidence: 99%

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Multi-photon excitation in uncaging the small molecule bioregulator nitric oxide

Garcia

Zhang

Ford

2013

Phil. Trans. R. Soc. A.

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Multi-photon excitation allows one to use tissue transmitting near-infrared (NIR) light to access excited states with energies corresponding to single-photon excitation in the visible or ultraviolet wavelength ranges. Here, we present an overview of the application of both simultaneous and sequential multi-photon excitation in studies directed towards the photochemical delivery (‘uncaging’) of bioactive small molecules such as nitric oxide (NO) to physiological targets. Particular focus will be directed towards the use of dyes with high two-photon absorption cross sections and lanthanide ion-doped upconverting nanoparticles as sensitizers to facilitate the uncaging of NO using NIR excitation.

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Section: Uncaging Using Upconversion Nanoparticles (A) Introduction Tmentioning

confidence: 99%

Section: Uncaging Using Upconversion Nanoparticles (A) Introduction Tmentioning

confidence: 99%

Multi-photon excitation in uncaging the small molecule bioregulator nitric oxide

Garcia

Zhang

Ford

2013

Phil. Trans. R. Soc. A.

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“…Apart from acting as a transducer to activate photocaged nucleic acids, the mesoporous silica coating on the surface of the UCNs help in the delivery of nucleic acids into the cells, protecting them from harmful enzymatic environment which might cause the degradation of nucleic acids. Lanthanide-based upconversion nanoparticles are also comparatively less toxic (18) and studies have proved them to be safe for long-term usage in vivo (19), thus providing a safe approach for use in biological applications. All the aforementioned properties make NIR-to-UV UCNs ideal candidates for in-depth control of biomolecules in vitro and in vivo.…”

mentioning

confidence: 99%

Remote activation of biomolecules in deep tissues using near-infrared-to-UV upconversion nanotransducers

Jayakumar

Idris

Zhang

2012

Proc. Natl. Acad. Sci. U.S.A.

344

255

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Controlled activation or release of biomolecules is very crucial in various biological applications. Controlling the activity of biomolecules have been attempted by various means and controlling the activity by light has gained popularity in the past decade. The major hurdle in this process is that photoactivable compounds mostly respond to UV radiation and not to visible or near-infrared (NIR) light. The use of UV irradiation is limited by its toxicity and very low tissue penetration power. In this study, we report the exploitation of the potential of NIR-to-UV upconversion nanoparticles (UCNs), which act as nanotransducers to absorb NIR light having high tissue penetration power and negligible phototoxicity and emit UV light locally, for photoactivation of caged compounds and, in particular, used for photo-controlled gene expression. Both activation and knockdown of GFP was performed in both solution and cells, and patterned activation of GFP was achieved successfully by using upconverted UV light produced by NIR-to-UV UCNs. In-depth photoactivation through tissue phantoms and in vivo activation of caged nucleic acids were also accomplished. The success of this methodology has defined a unique level in the field of photo-controlled activation and delivery of molecules.

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“…165,170 For in-vivo bioapplications, one of the major issues for upconversion nanocrystals is the fate of nanoparticles and potential toxicity of lanthanide ions. 187,188 Liu et al prepared 5.1 nm NaGdF 4 and 18.5 nm NaGdF 4 :Yb/Er nanoparticles with the same 45 surface modification and investigated their biodistributions in different organs and tissues of mice ( Figure 20). 189 The accumulation of both types of nanoparticles in liver decreased with the circulation time.…”

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confidence: 99%

Ultra-small fluorescent inorganic nanoparticles for bioimaging

et al. 2014

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Long-term in vivo biodistribution imaging and toxicity of polyacrylic acid-coated upconversion nanophosphors

Cited by 516 publications

References 37 publications

Multi-photon excitation in uncaging the small molecule bioregulator nitric oxide

Multi-photon excitation in uncaging the small molecule bioregulator nitric oxide

Remote activation of biomolecules in deep tissues using near-infrared-to-UV upconversion nanotransducers

Ultra-small fluorescent inorganic nanoparticles for bioimaging

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