Long‐term impact of puerperal metritis on the profiles of peripheral blood leukocytes in peripartum dairy cows

Magata, Fumie; Kitaoka, Ryuji; Morino, Ikumi; Teramura, Makoto; Kawashima, Chiho; Haneda, Shingo; Shimizu, Takashi

doi:10.1111/asj.12516

Cited by 10 publications

(5 citation statements)

References 21 publications

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“…Previous work has demonstrated an increase in the number of total lymphocytes during the periparturient period in cows with metritis, specifically two weeks prior to calving (Magata et al, 2016). Interestingly, these studies also demonstrated an increase in the number of CD8 + lymphocytes in the week preceding calving in opposition to our own data here, which suggests there is no difference in the proportion of prepartum CD3 + /CD8 + in cows that go on to develop uterine disease.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of peripheral blood mononuclear cell populations in periparturient dairy cows that develop metritis

Bromfield

Watt

Iacovides

2018

Veterinary Immunology and Immunopathology

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Bacterial contamination of the uterus following calving is ubiquitous in the dairy cow, 40% of cows develop postpartum uterine infection, including metritis. While predisposing factors like twinning and dystocia are associated with metritis, it is unclear why some cows remain healthy following calving and others develop uterine infection, negatively impacting animal health, milk production and economic return. Here, we profiled peripheral blood mononuclear cells of cows before calving and during postpartum metritis. We hypothesized that peripheral blood mononuclear cell function and proportions would be altered during the prepartum period in cows that develop postpartum metritis. Using flow cytometry we observed reduced proportions of peripheral CD3/CD4, CD4/CD62L, FOXP3 and CD21 populations from -10 to 40 days relative to calving associated with metritis, while the proportion of peripheral CD3/CD4 lymphocytes were specifically reduced in the prepartum period before the onset of metritis. Peripheral blood mononuclear cells from cows with metritis had a perturbed capacity to secrete IL-1β or IFNγ in response to in vitro stimulus; cells collected during the prepartum period from cows that would go on to develop metritis failed to increase IL-1β secretion in response to stimulation, while IFNγ secretion was altered at calving and postpartum in cows with metritis compared to healthy herd mates. No effect of metritis was observed in the capacity of cows to mount a humoral immune response to antigen administered on the day of calving. The studies discussed here suggest that while minor changes to the prepartum immune system are observed in cows that develop metritis, changes observed in the postpartum period are more prevalent and likely a consequences of disease and not causative. Future studies to modulate the prepartum immune system may help to limit postpartum metritis.

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Section: Discussionmentioning

confidence: 99%

Characterisation of peripheral blood mononuclear cell populations in periparturient dairy cows that develop metritis

Bromfield

Watt

Iacovides

2018

Veterinary Immunology and Immunopathology

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“…Uterine disorders, such as endometritis, are commonly observed in high-producing dairy cows and often cause poor reproductive performance, reduced milk yield, and consequently, result in economic loss ( 1 – 3 ). The endometrium is a natural barrier, and endometrial epithelial cells can resist the invasion of foreign pathogens by regulating the immune inflammatory response ( 4 ).…”

Section: Introductionmentioning

confidence: 99%

The Potential Therapeutic Role of miR-223 in Bovine Endometritis by Targeting the NLRP3 Inflammasome

et al. 2018

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Bovine endometritis affects milk production and reproductive performance in dairy cows and causes serious economic loss. The underlying molecular mechanisms or signaling pathways of bovine endometritis remain unclear. In this study, we attempted to determine the expression mechanism of mir-223 in endometritis of dairy cows and evaluate its potential therapeutic value. We first confirmed that there was an increased level of miR-223 in endometritis, and then, an LPS-induced bovine endometrial epithelial cell (BEND) line was used to mimic the inflammatory model in vitro. Our data showed that activation of NF-κB promoted the transcription of miR-223, thus inhibiting activation of the inflammatory mediator NLRP3 and its mediation of IL-1β production to protect against inflammatory damage. Meanwhile, in vivo studies showed that inhibition of mir-223 resulted in an enhanced pathology of mice during LPS-induced endometritis, while overexpression of mir-223 attenuated the inflammatory conditions in the uterus. In summary, our study highlights that miR-223 serves both to constrain the level of NLRP3 activation and to act as a protective factor in the inflammatory response and thus provides a future novel therapeutic modality for active flares in cow endometritis and other inflammatory diseases.

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“…Uterine diseases are prevalent in dairy cows, cause poor reproductive performance, reduced milk yield, and substantial economic losses 1 2 3 . Uterine diseases can be classified as puerperal metritis, clinical metritis, clinical endometritis and subclinical endometritis 4 5 6 .…”

mentioning

confidence: 99%

Circulating cell-free mature microRNAs and their target gene prediction in bovine metritis

Kasimanickam

Kastelic

2016

Sci Rep

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Uterine infections in dairy cows are common after calving, reduce fertility and cause substantial economic losses. Conventional diagnosis (based on clinical signs) and treatment can be challenging. Serum microRNA (miRNA) profiles serve as non-invasive biomarkers in several pathological conditions including inflammatory diseases. The objective was to identify differentially expressed serum miRNAs in cows with metritis and normal uterus (four cows per group), integrate miRNAs to their target genes, and categorize target genes for biological processes involved in bacterial infection and inflammatory responses. Out of 84 bovine-specific, prioritized miRNAs analyzed, 30 were differentially expressed between metritis and normal cows (p ≤ 0.05, fold regulation ≥2 magnitudes). Bta-miR-15b, bta-miR-17-3p, bta-miR-16b, bta-miR-148a, bta-miR-26b, bta-miR-101 and bta-miR-29b were highly up-regulated whereas bta-miR-148b, bta-miR-199a-3p, bta-miR-122, bta-miR-200b and bta-miR-10a were highly down-regulated in cows with metritis compared to cows with normal uterus. Highly scored target genes of up-regulated and down-regulated miRNAs were categorized for various biological processes, including biological regulation, cellular process, developmental process, metabolic process, localization, multicellular organismal process, response to stimulus, immune system process, cellular components organization, apoptotic process, biological adhesion, developmental process, and locomotion that are critical to combat bacterial infections and provoke inflammatory responses.

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Long‐term impact of puerperal metritis on the profiles of peripheral blood leukocytes in peripartum dairy cows

Cited by 10 publications

References 21 publications

Characterisation of peripheral blood mononuclear cell populations in periparturient dairy cows that develop metritis

Characterisation of peripheral blood mononuclear cell populations in periparturient dairy cows that develop metritis

The Potential Therapeutic Role of miR-223 in Bovine Endometritis by Targeting the NLRP3 Inflammasome

Circulating cell-free mature microRNAs and their target gene prediction in bovine metritis

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