Long-term <i>in vivo</i> CT tracking of mesenchymal stem cells labeled with Au@BSA@PLL nanotracers

Ning, Xinyu; Bao, Hongli; Liu, Xiaoyun; Fu, Han; Wang, Weizhi; Huang, Jie; Zhang, Zhijun

doi:10.1039/c9nr05637h

Cited by 33 publications

(30 citation statements)

References 49 publications

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“…The intracellular fluorescence intensity of FITC displayed a time‐ and dose‐dependent manner, in agreement with the results in a previous study (Figure 3b and Figure S8, Supporting Information). [ 31 ] To investigate the intracellular distribution of CPP‐PSD@Au, laser confocal microscopy was employed to image the labeled MSCs. As shown in Figure 3c, CPP‐PSD@Au was internalized and distributed in the cytoplasm of MSCs.…”

Section: Resultsmentioning

confidence: 99%

“…According to the previous reports, to achieve the effective dose of stem cells for IPF therapy, the number of the labeled MSCs was set at 1 × 10 6 to 4 × 10 6 cells and usually suspended in 50 to 350 µL (mm 3 ) PBS or normal saline (NS). [ 27,31,42 ] Therefore, the detection limit of the CPP‐PSD@Au labeled MSCs adequately met the imaging needs during stem cell‐based IPF therapy.…”

Section: Resultsmentioning

confidence: 99%

“…The successful induction of the model was confirmed by the behavior observation, weight variation, pathological section, and hydroxyproline (HYP) level change, which have been reported in our previous researches. [ 27,31 ] From the whole body CT image of the healthy mouse, the lung structure could be quickly and observed under the scanning condition at 60 kV, 133 µA with a scanning resolution of 50 µm (Figure 5b). After one day of transplantation, the labeled MSCs with a prominently enhanced CT contrast was observed at two horizontal levels, and could still be detectable in lung tissue at 35 days post‐transplantation (Figure 5c), manifesting that the CPP‐PSD@Au labeled MSCs could be successfully monitored by CT imaging for up to 35 days, much longer than the tracking time reported by other researchers.…”

Section: Resultsmentioning

confidence: 99%

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pH‐Triggered Aggregation of Gold Nanoparticles for Enhanced Labeling and Long‐Term CT Imaging Tracking of Stem Cells in Pulmonary Fibrosis Treatment

Chen

et al. 2021

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Gold nanoparticles (AuNPs) pose a great challenge in the development of nanotracers that can self‐adaptively alter their properties in response to certain cellular environments for long‐term stem cell tracking. Herein, pH‐sensitive Au nanotracers (CPP‐PSD@Au) are fabricated by sequential coupling of AuNPs with sulfonamide‐based polymer (PSD) and cell‐penetrating peptide (CPP), which can be efficiently internalized by mesenchymal stem cells (MSCs) and undergo pH‐induced self‐assembly in endosomes, facilitating long‐term computed tomography (CT) imaging tracking MSCs in a murine model of idiopathic pulmonary fibrosis (IPF). Using the CPP‐PSD@Au, the transplanted MSCs for the first time can be monitored with CT imaging for up to 35 days after transplantation into the lung of IPF mice, clearly elucidating the migration process of MSCs in vivo. Moreover, we preliminarily explored the mechanism of the CPP‐PSD@Au labeled MSCs in the alleviation of IPF, including recovery of alveolar integrity, decrease of collagen deposition, as well as down‐regulation of relevant cytokine level. This work facilitates our understanding of the behavior and effect of MSCs in the therapy of IPF, thereby providing an important insight into the stem cell‐based treatment of lung diseases.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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pH‐Triggered Aggregation of Gold Nanoparticles for Enhanced Labeling and Long‐Term CT Imaging Tracking of Stem Cells in Pulmonary Fibrosis Treatment

Chen

et al. 2021

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“…Bioluminescence imaging (BLI) which utilizes luciferase reactions also has the advantage of providing viability information of transplanted cells, but this method suffers from poor tissue penetration and low spatial resolution. MSCs can also be labeled with gold nanoparticle and tracked by computed tomography (CT) image in vivo [244,245]. These gold nanoparticles have advantage of exerting negligible influence on viability, proliferation, and differentiation ability of labeled MSCs, and offer good spatial resolution and long-term tracking when used in conjunction with CT modality [244].…”

Section: Methods Of Tracking Mscs In Vivomentioning

confidence: 99%

“…MSCs can also be labeled with gold nanoparticle and tracked by computed tomography (CT) image in vivo [244,245]. These gold nanoparticles have advantage of exerting negligible influence on viability, proliferation, and differentiation ability of labeled MSCs, and offer good spatial resolution and long-term tracking when used in conjunction with CT modality [244]. However, sensitivity is relatively poor, and there is still difficulty deriving quantitative information from CT images [246].…”

Section: Methods Of Tracking Mscs In Vivomentioning

confidence: 99%

Mesenchymal stem/stromal cell-based therapy: mechanism, systemic safety and biodistribution for precision clinical applications

et al. 2021

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Mesenchymal stem/stromal cells (MSCs) are a promising resource for cell-based therapy because of their high immunomodulation ability, tropism towards inflamed and injured tissues, and their easy access and isolation. Currently, there are more than 1200 registered MSC clinical trials globally. However, a lack of standardized methods to characterize cell safety, efficacy, and biodistribution dramatically hinders the progress of MSC utility in clinical practice. In this review, we summarize the current state of MSC-based cell therapy, focusing on the systemic safety and biodistribution of MSCs. MSC-associated risks of tumor initiation and promotion and the underlying mechanisms of these risks are discussed. In addition, MSC biodistribution methodology and the pharmacokinetics and pharmacodynamics of cell therapies are addressed. Better understanding of the systemic safety and biodistribution of MSCs will facilitate future clinical applications of precision medicine using stem cells.

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A Neurospheroid‐Based Microrobot for Targeted Neural Connections in a Hippocampal Slice

Kim¹,

Jeon²,

Yang

et al. 2023

Advanced Materials

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Functional restoration by the re‐establishment of cellular or neural connections remains a major challenge in targeted cell therapy and regenerative medicine. Recent advances in magnetically powered microrobots have shown potential for use in controlled and targeted cell therapy. In this study, a magnetic neurospheroid (Mag‐Neurobot) that can form both structural and functional connections with an organotypic hippocampal slice (OHS) is assessed using an ex vivo model as a bridge toward in vivo application. The Mag‐Neurobot consists of hippocampal neurons and superparamagnetic nanoparticles (SPIONs); it is precisely and skillfully manipulated by an external magnetic field. Furthermore, the results of patch‐clamp recordings of hippocampal neurons indicate that neither the neuronal excitabilities nor the synaptic functions of SPION‐loaded cells are significantly affected. Analysis of neural activity propagation using high‐density multi‐electrode arrays shows that the delivered Mag‐Neurobot is functionally connected with the OHS. The applications of this study include functional verification for targeted cell delivery through the characterization of novel synaptic connections and the functionalities of transported and transplanted cells. The success of the Mag‐Neurobot opens up new avenues of research and application; it offers a test platform for functional neural connections and neural regenerative processes through cell transplantation.

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Long-term in vivo CT tracking of mesenchymal stem cells labeled with Au@BSA@PLL nanotracers

Abstract: Au@BSA@PLL nanotracers were developed for long-term CT tracking of hMSCs in the treatment of pulmonary fibrosis injury.

Cited by 33 publications

References 49 publications

pH‐Triggered Aggregation of Gold Nanoparticles for Enhanced Labeling and Long‐Term CT Imaging Tracking of Stem Cells in Pulmonary Fibrosis Treatment

pH‐Triggered Aggregation of Gold Nanoparticles for Enhanced Labeling and Long‐Term CT Imaging Tracking of Stem Cells in Pulmonary Fibrosis Treatment

Mesenchymal stem/stromal cell-based therapy: mechanism, systemic safety and biodistribution for precision clinical applications

A Neurospheroid‐Based Microrobot for Targeted Neural Connections in a Hippocampal Slice

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