Long-term HIV-1 infection of neural progenitor populations

Abstract

These studies suggest that neural progenitor populations can contribute to the reservoir for HIV in the brain and undergo changes as a consequence of HIV persistence.

Transduction of human neural progenitor cells with foamy virus vectors for differentiation-dependent gene expression

“…24 We have demonstrated earlier that HNSC.100 populations cultured in the astrocyte-differentiation medium for 7 days adopt morphological features of astrocytes and show strongly increased immunoreactivity for GFAP antigen, compared with HNSC.100 populations cultured in the proliferation medium. 25 Here we show by real-time PCR analysis that levels of gfap RNA are strongly increased in HNSC.100 astrocyte populations compared with progenitor populations (66 784-fold increase) (Figure 1), confirming differentiation-dependent activation of the gfap promoter. To verify the differentiation of neural progenitor cells to astrocytes, a second astrocyte marker gene was analysed by real-time PCR analysis.…”

“…25 Here we demonstrate that a FV vector carrying a transgene with the Gfa2 promoter and sequences encoding the enhanced green fluorescent protein (EGFP) (Gfa2-egfp) can stably transduce HNSC.100 cells. We demonstrate that the expression of the Gfa2-egfp transgene is activated by astrogenesis of transduced HNSC.100 cells.…”

“…For differentiation into astrocytes, HNSC.100 were cultured in basal medium supplemented with 100 ng ml À1 of ciliary neurotrophic factor (CNTF) (that is, astrocyte-differentiation medium) for at least 7 days. 25 Basic fibroblast growth factor, epidermal growth factor and CNTF were purchased from Tebu-Bio (Offenbach, Germany) and all other cell culture reagents from Invitrogen (Karlsruhe, Germany).…”

Coxsackievirus Preferentially Replicates and Induces Cytopathic Effects in Undifferentiated Neural Progenitor Cells

“…Also, the migratory nature of immature neuroblasts may assist in virus dissemination within the brain following infection of progenitor or stem cells (15). Recently, both human cytomegalovirus (7,27), HIV-1 (31,34), and Japanese encephalitis virus (9,10) have been shown to target neural stem cells and may influence stem cell function (26,29,30) and immunogenicity (11).…”

EASY-HIT: HIV Full-Replication Technology for Broad Discovery of Multiple Classes of HIV Inhibitors

“…For Gag p24 staining, 1 ϫ 10 6 LC5-RIC cells were fixed, washed, and permeabilized as previously described (49). Immunostaining with a fluorescein isothiocyanate (FITC)-labeled p24-specific antibody, KC57-FITC (Becton Dickinson, Heidelberg, Germany), was carried out for 2 h in the dark, followed by three washing steps with phosphatebuffered saline (PBS) and resuspension of cells in PBS for further analysis.…”