Long‐term hematologic reconstitution after autologous peripheral blood progenitor cell transplantation: a comparison between controlled‐rate freezing and uncontrolled‐rate freezing at 80°C

Montanari, Mauro; Capelli, Debora; Poloni, Antonella; Massidda, Danilo; Brunori, Marino; Spitaleri, Luca; Offidani, Massimo; Lucesole, Moira; Masia, Maria Cristiana; Balducci, F; Refè, C.; Piani, M; Leoni, Pietro; Olivieri, Attilio

doi:10.1046/j.1537-2995.2003.00271.x

Cited by 35 publications

(35 citation statements)

References 18 publications

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“…Previous reports found a similar slightly slower hematopoietic recovery when comparing stem cell cryopreservation with DMSO with or without HES, 13 with or without controlled-rate freezing 14 as well as with different concentrations of DMSO. 15 More importantly, two incidences of poor or incomplete engraftments occurred when Group DMSO 5% Group DMSO 10%…”

Section: Outcome and Long-term Follow-up Datasupporting

confidence: 59%

Long-term hematologic reconstitution and clinical evaluation of autologous peripheral blood stem cell transplantation after cryopreservation of cells with 5% and 10% dimethylsulfoxide at 80 C in a mechanical freezer

Galmés¹,

Gutiérrez²,

Sampol³

et al. 2007

Haematologica

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We report the long-term evaluation over 12 years of a simplified technique for stemcell cryopreservation at -80ºC without rate-controlled freezing and with 5% (n=251) or 10% (n=47) DMSO as the sole cryoprotectant. Platelet recovery was greater in the 5% DMSO group while long-term hematologic recovery did not differ. Factors influencing a faster hematologic recovery were infusion of more than 2.7×10 6 /kg of CD34 + cells, 10% DMSO cryopreservation and G-CSF. We confirm that the procedure is feasible with a reduction in infusion-related toxicity from 60% using 5% DMSO. Differences in hematologic reconstitution were not clinically significant if a minimum of 1. 1 The toxic effects related to DMSO infusion are generally dose-related and while they are usually mild, they can become severe.2-4 HES is a relatively non-toxic drug but it is related with long-lasting pruritus 5 and osmotic nephrotoxicity. Cryopreservation protocols usually involve ratecontrolled freezing followed by the storage of the HSC in either the liquid or vapor phase of liquid nitrogen. These procedures are time consuming and require expensive computerassisted devices.

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Section: Outcome and Long-term Follow-up Datasupporting

confidence: 59%

Long-term hematologic reconstitution and clinical evaluation of autologous peripheral blood stem cell transplantation after cryopreservation of cells with 5% and 10% dimethylsulfoxide at 80 C in a mechanical freezer

Galmés¹,

Gutiérrez²,

Sampol³

et al. 2007

Haematologica

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“…The procedures are both expensive and time-consuming. As an alternative, a simple method of cryopreservation using a mechanical freezer has been introduced for the PBSCT procedure (Makino et al 1991;Takaue et al 1994;Cilloni et al 1999;Choi et al 2001;Montanari et al 2003). One important disadvantage of uncontrolled-rate freezing is the lack of recordable data on the cooling rate.…”

Section: Discussionmentioning

confidence: 99%

“…As an alternative, several groups have collaborated in the development of a simple method for the cryopreservation of PBPCs in a -85˚C mechanical freezer for autologous peripheral blood stem cell transplantation (PBSCT). Some studies on simple cryopreservation methods have shown reproducible hematopoietic recoveries after PBSCT (Makino et al 1991;Takaue et al 1994;Cilloni et al 1999;Choi et al 2001;Montanari et al 2003). Our group recently provided a freezing rate of approximately 1˚C/min for cord blood (CB) cryopreservation by storing the freezing bag in a protective aluminum canister wrapped with heatinsulating materials and placing sample tubes in a double styrene foam box inside a -85˚C electric freezer .…”

mentioning

confidence: 99%

Evaluation of Hematological Reconstitution Potential of Autologous Peripheral Blood Progenitor Cells Cryopreserved by a Simple Controlled-Rate Freezing Method

Kudo

Minegishi

Itoh

et al. 2005

Tohoku J. Exp. Med.

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A novel and simple procedure for the controlled-rate cryopreservation of peripheral blood progenitor cells (PBPCs) was introduced. A freezing bag housed in a protective aluminum canister was placed on top of a styrene foam box in the -85˚C electric freezer. A second set of samples was kept in cryotubes placed in a double styrene foam box in the same electric freezer. Measurement of the freezing rate in the PB bags and cryotubes demonstrated that this simple method for PBPC cryopreservation provided optimal conditions for both large-scale and small-scale cryopreservation. Within several days after autologous peripheral blood stem cell transplantation, we thawed the cells in the small sample tubes and evaluated the cell viability, the cell recovery, and the recovery rates of hematopoietic progenitor cells (HPCs), such as CD34 + cells and colonyforming unit-granulocyte/macrophage (CFU-GM) colonies. The median duration of cryopreservation was 59 days (range, 14 -365 days). According to our analysis, infusions of more than 2 × 10 6 CD34 + cells/kg body weight and 0.5 × 10 6 CFU-GM colonies/kg body weight after thawing had favorable influences on the neutrophil engraftment. We have therefore established a simple freezing method for cryopreservation of human PBPCs, which ensures the transplantability of hematopoietic progenitors even after thawing. In vitro HPC assay after thawing is important to evaluate the quality of cryopreservation procedures.peripheral blood progenitor cell; CD34 + cell; CFU-GM colony; cryopreservation

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“…However, the inevitable loss of CD34 þ cells between collection and transplant, especially during cryopreservation, is difficult to predict without assessment of the sample after cryopreservation. 4,5,7,8,18 This leads to the current debate as to whether the quantification of HPC should be performed before or after cryopreservation.…”

Section: Cd34mentioning

confidence: 99%

“…Investigators 4,5,7,8,18 who have studied post-thaw CD34 þ cells and hematopoietic recovery have found that there was a significant link between the number of CD34 þ cells after cryopreservation and neutrophil and platelet engraftment. It has been proposed 6 that the number of CD34 þ cells should be determined after cryopreservation, and that at least the loss of HPC should be monitored.…”

Section: Cd34mentioning

confidence: 99%

Association of post-thaw viable CD34+ cells and CFU-GM with time to hematopoietic engraftment

Yang

Acker

Cabuhat

et al. 2005

Bone Marrow Transplant

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Summary:In all, 78 peripheral hematopoietic progenitor cell collections from 52 patients were evaluated using our previously published validated post-thaw assays at the time of collection and following transplantation by assessment of viable CD34 þ cells, and granulocytemacrophage colony-forming units (CFU-GM) cryopreserved in quality control vials. The median (range) post-thaw recovery of viable CD34 þ cells and CFU-GM was 66.4% (36.1-93.6%) and 63.0% (28.6-85.7%), respectively, which did not show significant correlation with the engraftment of either neutrophils (P ¼ 0.136 and 0.417, respectively) or platelets (P ¼ 0.88 and 0.126, respectively). However, the reinfused viable CD34 þ cells/ kg of patient weight pre-or post-cryopreservation showed significant correlation to engraftment of neutrophils (P ¼ 0.0001 and 0.001, respectively) and platelets (P ¼ 0.023 and 0.010, respectively), whereas CFU-GM pre-or post-cryopreservation was significantly correlated to neutrophils (P ¼ 0.011 and 0.007, respectively) but not to platelets (P ¼ 0.112 and 0.100, respectively). The results show that post-cryopreservation assessment of viable CD34 þ cells or CFU-GM is as reliable a predictor of rapid engraftment as that of pre-cryopreservation measures. Therefore, the post-cryopreservation number of viable CD34 þ cells or CFU-GM should be used to eliminate the risks of unforeseen cell loss that could occur during cryopreservation or long-term storage.

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Long‐term hematologic reconstitution after autologous peripheral blood progenitor cell transplantation: a comparison between controlled‐rate freezing and uncontrolled‐rate freezing at 80°C

Abstract: Our study confirms that URF is safe and allows sustained long-term engraftment without increasing the risks of transplantation, even though the early engraftment after URF is slower.

Cited by 35 publications

References 18 publications

Long-term hematologic reconstitution and clinical evaluation of autologous peripheral blood stem cell transplantation after cryopreservation of cells with 5% and 10% dimethylsulfoxide at 80 C in a mechanical freezer

Long-term hematologic reconstitution and clinical evaluation of autologous peripheral blood stem cell transplantation after cryopreservation of cells with 5% and 10% dimethylsulfoxide at 80 C in a mechanical freezer

Evaluation of Hematological Reconstitution Potential of Autologous Peripheral Blood Progenitor Cells Cryopreserved by a Simple Controlled-Rate Freezing Method

Association of post-thaw viable CD34+ cells and CFU-GM with time to hematopoietic engraftment

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