Long-term follicular dynamics and biochemical characteristics of dominant follicles in dairy cows subjected to acute heat stress

Güzeloğlu, Aydın; Ambrose, D.J.; Kassa, Tesfu; Díaz, Thais; Thatcher, M.-J.; Thatcher, W.W.

doi:10.1016/s0378-4320(01)00082-3

Cited by 71 publications

(53 citation statements)

References 35 publications

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“…This effect is in line with previous reports (Theodorakis and Morimoto 1987;Trinklein et al 2004;Akerfelt et al 2007) that heat stress can induce production of HSPs by non-ovarian cells, but not with reports on lack of effect of heat stress on ovarian HSP90 (Guzeloglu et al 2001). The ability of high temperatures to induce both HSP mRNAs accumulation and HSP depletion suggest that thermal stress can promote both HSP synthesis and metabolisation by ovarian cells.…”

Section: Discussionsupporting

confidence: 90%

“…This is the first demonstration of synthesis (mRNA) of HSPs in porcine ovarian cells, as well as the confirmation of accumulation of HSP70 in porcine ovary observed previously in other species (Driancourt et al 1999;Guzeloglu et al 2001;Narayansingh et al 2004;Maniwa et al 2005;Salvetti et al 2008). The presence of HSP in both granulosa cells and whole follicle confirm previous indications (Driancourt et al 1999) that HSP is produced in both granulosa and theca compartments but predominantly in granulosa cells.…”

Section: Discussionsupporting

confidence: 88%

“…Negative effects of suboptimal temperatures on reproductive processes are well documented (Putney et al 1989;Edwards and Hansen 1997;Guzeloglu et al 2001;Lawrence et al 2004), but the mechanisms of such effect remain not fully known. It is demonstrated that high temperatures can affect reproductive processes via inhibition of both ovarian cell proliferation, apoptosis, induction of oversecretion of ovarian steroid hormones and alteration in response of cultured ovarian cells to hormones.…”

Section: Introductionmentioning

confidence: 99%

“…Since HSP70 is present in the ovary (Driancourt et al 1999;Guzeloglu et al 2001;Narayansingh et al 2004;Maniwa et al 2005;Salvetti et al 2008), it is not to be excluded that heat shock can affect reproduction, like other processes, via HSPs. Nevertheless, acute heat stress did not alter HSP90 level in bovine ovarian follicular fluid (Guzeloglu et al 2001) and effect of high temperatures on ovarian HSPs, and involvement of HSPs in ovarian response to heat stress has not been demonstrated.…”

Section: Introductionmentioning

confidence: 99%

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Heat shock proteins in porcine ovary: synthesis, accumulation and regulation by stress and hormones

Sirotkin

Bauer

2011

Cell Stress and Chaperones

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The present studies aimed to understand the interrelationships between stress, hormones and heat shock proteins (HSPs) in the ovary. We examined (1) whether HSP70.2, HSP72 and HSP105/110 can be produced and accumulated in porcine ovarian tissue, (2) whether these HSPs could be indicators of stress, i.e. whether two kinds of stress (high temperatures and malnutrition/serum deprivation) can affect them, and (3) whether some hormonal regulators of ovarian functions (insulin-like growth factor (IGF)-I, leptin and follicle-stimulating hormone (FSH)) can affect these HSPs and response of ovaries to HSP-related stress. We analysed the expression of HSP70.2, HSP72 and HSP105/110 mRNA (by using real-time reverse transcriptase polymerase chain reaction) in porcine ovarian granulosa cells, as well as the accumulation of HSP70 protein (by using sodium dodecyl sulphate polyacrylamide gel electrophoresis-Western) in either whole ovarian follicles and granulose cells cultured at normal (37.5°C) or high (41.5°C) temperature, with and without serum and with and without IGF-I, leptin and FSH. Expression of mRNA for HSP70.2, HSP72 and HSP105/110 in ovarian granulosa cells and accumulation of HSP70 protein in whole ovarian follicles and granulosa cells were demonstrated. In all the groups, addition of either IGF-I, leptin and FSH reduced the expression of HSP70.2, HSP72 and HSP105/110 mRNA. Both high temperature, serum deprivation and their combination resulted in increase in mRNAs for all three analysed HSPs. Additions of either IGF-I, leptin and FSH prevented the stimulatory effect of both high temperature and serum deprivation on the transcription of HSP70.2, HSP72 and HSP105/110. In contrast, high temperature reduced accumulation of peptide HSP70 in both ovarian follicles and granulosa cell. Serum deprivation promoted accumulation of HSP70 in granulosa cells, but not in ovarian follicles. Addition of IGF-I, leptin and FSH was able to alter accumulation of HSP70 in both follicles and granulosa cells. The present observations suggest (1) that HSPs can be synthesised in ovarian follicular granulosa cells; (2) that hormones (IGF-I, leptin and FSH) can inhibit, whilst stressors (both high temperature and malnutrition/serum deprivation) can stimulate transcription of HSP70.2, HSP72 and HSP105/ 110 genes, whilst heat stress, but not malnutrition, can promote depletion of HSP70 in ovarian cells, and (3) that hormones (IGF-I, leptin and FSH) can prevent stress-related changes in HSPs. The application of HSPs as indicators and mediators of stress and hormones on ovarian functions, as well as use of hormones and HSPs as anti-stressor molecules, are discussed.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Heat shock proteins in porcine ovary: synthesis, accumulation and regulation by stress and hormones

Sirotkin

Bauer

2011

Cell Stress and Chaperones

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“…Heat stress reduces gonadotropin-releasing hormone (GnRH) and LH secretion (Madan and Johnson, 1973;Wise et al, 1988;Gilad et al, 1993) and the degree of dominance of the selected follicle, it reduced the steroidogenic capacity of theca and granulosa cells and it caused low blood estradiol concentrations (Badinga et al, 1993;Wolfenson et al, 1995 andRoth et al, 2000). The reported effects of heat stress on plasma progesterone concentrations in dairy cows show marked discrepancies: studies have reported increased (Abilay et al, 1975;Trout et al, 1998;Wilson et al, 1998), decreased (Rosenberg et al, 1977;Jonsson et al, 1997;Ronchi et al, 2001) or unchanged (Roth et al, 2000;Guzeloglu et al, 2001) concentrations of plasma progesterone during the warm season (for review see De Rensis and Scaramuzzi, 2003). These endocrine changes are associated with reduced follicular activity, altered ovulatory mechanisms and decreased oocyte and embryo quality.…”

mentioning

confidence: 99%

Effect of season on luteal activity during the post partum period of dairy cows in temperate areas

Rensis

López‐Gatius

Capelli

et al. 2008

Animal

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Seasonal effects on luteal activity during post partum were evaluated in two consecutive studies in 253 dairy cows in Northern Italy. In study 1, plasma progesterone concentrations were determined on days 14, 21, 28, 35, 42, 49 and 56 post partum and in study 2 cows were synchronized and inseminated at a fixed time using two regimes based on the 'Ovsynch' protocol. Study 1: Animals were classified as luteal (progesterone .1.5 ng/ml in at least two consecutive samples) or non-luteal (progesterone ,1.5 ng/ml in all samples). The proportion of cows without luteal activity from calving to day 56 post partum was 47/253 (18.5%). Of the 47 cows without luteal activity, 42 (89%) were detected during the warm months of the year and five were detected during the cold months of the year, and the effect of season was highly significant (P , 0.001). Study 2: Three study groups were established; control (CONT, untreated cows, n 5 92), GPG (cows receiving gonadotropin-releasing hormone (GnRH) on day 0, PGF 2a on day 7 followed by a second dose of GnRH 24 h later, n 5 80); and HPH (the same as the GPG group, but with human chorionic gonadotropin (hCG) substituted for GnRH, n 5 81). In the GPG and HPH groups, cows were inseminated 16 to 22 h after the second GnRH or hCG injection. Untreated cows were inseminated at first estrus after a voluntary weaning period. Because the effects of the GPG and HPH regimes on pregnancy rate were not significantly different, data were pooled into a single treatment group (TREAT). Pregnancy rates during the warm months of the year were 16% and 15% at first service and 65% and 66% at day 135 post partum for CONT and TREAT groups, respectively. Pregnancy rates during the cold months of the year were 36% and 38% at first service and 72% and 76% at day 135 post partum for CONT and TREAT groups, respectively. There was an effect of season (P , 0.05) but not of treatment on pregnancy rate. Treatment reduced the number of days from calving to conception during both the cold (101 6 3.2 v. 121 6 3.1 days; P , 0.001) and warm seasons (122 6 3.2 v. 145 6 3.1 days; P , 0.001). In conclusion, the present study shows that (i) heat stress during the warm season can compromise luteal activity and (ii) that regimes based on the Ovsynch protocol did not improve pregnancy rate at first service or by 135 post partum, but they had a positive effect on the calving-to-conception interval.Keywords: progesterone, heat stress, season, dairy cow, Ovsynch IntroductionPost partum anestrous persisting beyond 50 to 60 days after calving is common in high-producing dairy cows and it is one of the main factors that increases the length of the inter-calving interval (Opsomer et al., 2000;Lamming and Royal, 2001;Ló pez-Gatius et al., 2001). This problem can be caused either by a failure of cyclic cows to show estrous behavior or by a failure of the cows to re-commence cycling after calving. Cows can remain acyclic because of the presence of persistent follicular cysts, persistent corpora lutea or inactive ovaries with no follicul...

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Strategies for Improvement of Thermal and Reproductive Responses under Heat Stress

Wolfenson

Thatcher

2012

Environmental Physiology of Livestock

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Long-term follicular dynamics and biochemical characteristics of dominant follicles in dairy cows subjected to acute heat stress

Cited by 71 publications

References 35 publications

Heat shock proteins in porcine ovary: synthesis, accumulation and regulation by stress and hormones

Heat shock proteins in porcine ovary: synthesis, accumulation and regulation by stress and hormones

Effect of season on luteal activity during the post partum period of dairy cows in temperate areas

Strategies for Improvement of Thermal and Reproductive Responses under Heat Stress

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