Long‐term field insecticide susceptibility data and laboratory experiments reveal evidence for cross resistance to other neonicotinoids in the imidacloprid‐resistant brown planthopper <i>Nilaparvata lugens</i>

Fujii, Tomohisa; Sanada‐Morimura, Sachiyo; Oe, Takaho; Ide, Manami; Thanh, Dinh Van; Chien, Ho Van; Tuong, Phan Van; Loc, Phung Minh; Cuong, Le Quoc; Liu, Zewen; Zhu, Zhiliang; Li, Jianhong; Wu, Gang; Huang, Sheng-Lung; Estoy, Gerardo F; Sonoda, Shoji; Matsumura, Masaya

doi:10.1002/ps.5533

Cited by 26 publications

(14 citation statements)

References 31 publications

(93 reference statements)

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“…Imidacloprid‐resistant strains were collected in 2009 (VN09‐1 and VN09‐3) and 2010 (VN10‐1) in Vietnam and 2014 in the Kumamoto prefecture, Japan (KR‐14). VN09‐3_F 24 was the most resistant laboratory‐selected strain established from VN09‐3 with exposure to imidacloprid for 24 generations 20–22 . Izumo‐87♀ x VN09‐1♂_F 1 (IxV‐F 1 ) and Izumo‐87♂ x VN09‐1♀_F 1 (VxI‐F 1 ) were F 1 s of the VN09‐1 and Izumo‐87 strains.…”

Section: Methodsmentioning

confidence: 99%

“…VN09-3_F 24 was the most resistant laboratory-selected strain established from VN09-3 with exposure to imidacloprid for 24 generations. [20][21][22] Izumo-87♀ x VN09-1♂_F 1 (IxV-F 1 ) and Izumo-87♂ x VN09-1♀_F 1 (VxI-F 1 ) were F 1 s of the VN09-1 and Izumo-87 strains. All insects were reared on rice seedlings in a glass bottle (6.5 cm diameter, 18 cm height) covered with a cloth mesh at 26°C under a long-day photoperiod (16L:8D) without exposure to insecticides.…”

Section: Insectsmentioning

confidence: 99%

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Genome‐wide assessment and development of molecular diagnostic methods for imidacloprid‐resistance in the brown planthopper, Nilaparvata lugens (Hemiptera; Delphacidae)

Yokoi

Nakamura

Jouraku

et al. 2020

Pest Management Science

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BACKGROUND The brown planthopper, Nilaparvata lugens (Stål), is one of the most notorious pests of rice throughout Asia. The brown planthopper has developed high resistance to imidacloprid, a member of neonicotinoid insecticides. Several genes and mutations conferring imidacloprid resistance in N. lugens, especially in eastern and southeastern Asia populations, have been reported. Thus, the key mechanisms of imidacloprid resistance need to be examined. RESULTS RNA‐seq analyses revealed that only one cytochrome P450 monooxygenase gene, CYP6ER1, was commonly upregulated in the five resistant strains tested. Sequences of CYP6ER1, which were highly expressed in the imidacloprid‐resistant strains, contained a three‐nucleotide deletion in the coding region, and amino acid substitutions and deletion, compared to that in an imidacloprid‐susceptible strain. RNAi‐mediated gene knockdown of CYP6ER1 increased imidacloprid susceptibility in a resistant strain. Further, we established two simple and convenient PCR‐based molecular diagnostic methods to detect the CYP6ER1 locus with the three‐nucleotide deletion. Using these methods, the resistance of F2 progenies derived from the crosses of F1 siblings from susceptible and resistant parents was analyzed, showing that the imidacloprid resistance had a relationship to the CYP6ER1 locus with the three‐nucleotide deletion. CONCLUSION The overexpression of a variant CYP6ER1 with amino acid substitutions and deletion was involved in imidacloprid resistance in N. lugens. Based on these findings, molecular diagnostic methods have been developed and are promising tools for monitoring imidacloprid resistance in paddy fields. © 2020 Society of Chemical Industry

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Section: Methodsmentioning

confidence: 99%

Section: Insectsmentioning

confidence: 99%

Genome‐wide assessment and development of molecular diagnostic methods for imidacloprid‐resistance in the brown planthopper, Nilaparvata lugens (Hemiptera; Delphacidae)

Yokoi

Nakamura

Jouraku

et al. 2020

Pest Management Science

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“…- Saeed et al (2018) found in Pakistan that there was very high resistance to acetamiprid (433-fold) and imidacloprid (173-fold) in the crop pest Dysdercus koenigii. -Fujii et al (2019) studied resistance in the brown planthopper in East Asia and Vietnam. Initially this was with imidacloprid, but this had spread to thiamethoxam and clothianidin, but not to dinotefuran and nitenpyram.…”

Section: Effectiveness and Alternativesmentioning

confidence: 99%

Social, Psychological and Health Impact of Coronavirus Disease (COVID-19) on the Elderly: South African and Italian Perspectives

2021

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Agriculture is critically important for African societies and economies but ensuring food security for Africa’s growing population is a major challenge. One particular concern are pesticides called ‘neonicotinoids’, which render all parts of a plant toxic to all insects and contaminate the soil and water bodies. By exposing all organisms to the toxins, neonicotinoids also harm beneficial insects that provide many important ‘ecosystem services’, such as pollination, soil development, and natural pest control, which are an integral part of sustainable agriculture. Neonicotinoids have contributed to the loss of ecosystem services from pollinators and other insects in Europe and elsewhere, and several of them have been banned in the (European Union) EU and other countries due to their harmful effect on beneficial insects. Africa, with its rich biodiversity and heavy reliance on agricultural production, is one of the fastest-growing pesticide markets in the world, so protecting it from the harmful effects of neonicotinoids is vital to ensuring a sustainable agriculture that provides food security. The Academy of Science of South Africa (ASSAf), in collaboration with the German National Academy of Sciences Leopoldina and the Network of African Science Academies (NASAC) has recently completed a project exploring the use and effects of neonicotinoids in African agriculture. This project brought together experts from 17 African countries, reviewed the relevant African scientific literature, and analysed the state of knowledge on neonicotinoids and their impact on ecosystem services for agriculture and on biodiversity in Africa. The resultant report ‘Neonicotinoid insecticides: use and effects in African agriculture. A review and recommendations to policy makers’ (NASAC, 2019) has collated an unprecedented amount of information, identified gaps in scientific knowledge and research relating to neonicotinoids in Africa, and developed key recommendations from science to policy-makers to ensure the sustainability of African agriculture and thus food security. One year after the launch of the NASAC report, the purpose of this virtual event was to introduce the report, including an update on recent global scientific and African policy developments regarding neonicotinoids, and to discuss its implications with a wide range of stakeholders, with the aim of stimulating policy and research action on this important issue. The target audience and participants included South African and Southern African Development Community (SADC) policymakers, regulatory agencies, government departments, agricultural associations, extension-service providers, research institutes, international development agencies, representatives of embassies, and other interested stakeholders.

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“…Currently, chemical control is the main measure implemented to prevent BPH outbreaks. Yet due to the excessive and unscientific use of insecticides, BPH has since developed resistance to many of them 6,7 . Cultivating resistant rice varieties is the most economical and environmentally friendly method to control BPH populations; however, this pest may gradually adapt and persist after being reared on resistant rice plants for multiple generations 8,9 .…”

Section: Introductionmentioning

confidence: 99%

RNAi‐mediated silencing of the autophagy‐related gene NlATG3 inhibits survival and fecundity of the brown planthopper, Nilaparvata lugens

Feng

et al. 2021

Pest Management Science

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BACKGROUND The brown planthopper (BPH), Nilaparvata lugens, is a serious insect pest of rice. Autophagy and its related gene ATG3 play multiple roles in insects. However, information about the functions of ATG3 in BPH (NlATG3) is unavailable, and its potential as a target for pest control remains unclear. RESULTS RT‐qPCR results showed a relatively low expression of NlATG3 in 1st–4th‐instar nymphs, which increased through 9‐day‐old adults. The expression of NlATG3 increased continuously in 1‐day‐old through 5‐day‐old eggs, whereas it decreased thereafter. The mRNA level of NlATG3 was markedly higher in the ovary (1.16) and head (1.00) compared to the rest body parts of BPH adults. Injecting nymphs with dsNlATG3 at doses from 62.5 to 250 ng per insect had strong lethal effect upon them. For the 5th‐instar nymphs, all individuals died within 5 days after receiving the dsNlATG3, and importantly, no individual successfully molted. Transmission electron microscopy revealed the new cuticle of nymphs injected with dsNlATG3 became loose and curved, which is clearly different from that of the control. Correspondingly, the obvious vesicles in epidermal cells disappeared after dsNlATG3‐treatment. RNAi of NlATG3 significantly reduced the total number of eggs laid per female as well as the eggs' hatchability, especially in the dsNlATG3♀ × dsGFP♂ group, whose total number of eggs laid per female largely decreased by 80.4%, and whose eggs' hatchability was significantly reduced from 95.7% to zero, when compared with the control (dsGFP♀ × dsGFP♂). CONCLUSION NlATG3 is a promising target for developing RNAi‐based insect management strategies. © 2021 Society of Chemical Industry.

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Long‐term field insecticide susceptibility data and laboratory experiments reveal evidence for cross resistance to other neonicotinoids in the imidacloprid‐resistant brown planthopper Nilaparvata lugens

Cited by 26 publications

References 31 publications

Genome‐wide assessment and development of molecular diagnostic methods for imidacloprid‐resistance in the brown planthopper, Nilaparvata lugens (Hemiptera; Delphacidae)

Genome‐wide assessment and development of molecular diagnostic methods for imidacloprid‐resistance in the brown planthopper, Nilaparvata lugens (Hemiptera; Delphacidae)

Social, Psychological and Health Impact of Coronavirus Disease (COVID-19) on the Elderly: South African and Italian Perspectives

RNAi‐mediated silencing of the autophagy‐related gene NlATG3 inhibits survival and fecundity of the brown planthopper, Nilaparvata lugens

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