Long-term Extensive Expansion of Mouse Hepatic Stem/Progenitor Cells in a Novel Serum-Free Culture System

Tsuchiya, Atsunori; Heike, Toshio; Fujino, Hisanori; Shiota, Mitsutaka; Umeda, Katsutsugu; Yoshimoto, Momoko; Matsuda, Yasunobu; Ichida, Takafumi; Aoyagi, Yoshinobu; Nakahata, Tatsutoshi

doi:10.1053/j.gastro.2005.03.030

Cited by 40 publications

(56 citation statements)

References 39 publications

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“…The polygonal epithelial cells from single aggregates proliferated rapidly and formed moderate colonies within 4-5 days (Supplementary information, Figure S2A) and large colonies within 8-10 days (Supplementary information, Figure S2B) consisting of thousands of cells with similar morphology to previously identified HPCs [5,6]. Almost all of the polygonal epithelial cells in different colonies were positive for E-cad ( Figure 1B-i), a marker of HPCs [7,8].…”

Section: Dear Editorsupporting

confidence: 54%

Isolation and characterization of liver epithelial progenitor cells from normal adult rhesus monkeys (Macaca mulatta)

Jin

Tang

et al. 2009

Cell Res

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Section: Dear Editorsupporting

confidence: 54%

Isolation and characterization of liver epithelial progenitor cells from normal adult rhesus monkeys (Macaca mulatta)

Jin

Tang

et al. 2009

Cell Res

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“…To mimic cell-matrix interactions, culture dishes can be coated with matrix components. Collagen type I supports the long-term colony-forming ability of mouse liver progenitors, 69 laminin-5 causes osteogenic differentiation in human BMSCs, 70 and fibronectin inhibits differentiation of human adipose stem cells and progenitors. 71 Physiological factors, including oxygen tension and pH, are also relevant in the stem cell environment.…”

Section: Cultivation Strategiesmentioning

confidence: 98%

Review:Ex VivoEngineering of Living Tissues with Adult Stem Cells

et al. 2006

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Adult stem cells have the potential to revolutionize regenerative medicine with their unique abilities to self-renew and differentiate into various phenotypes. This review examines progress and challenges in ex vivo tissue engineering with adult stem cells. These rare cells are harvested from a variety of tissues, including bone marrow, adipose, skeletal muscle, and placenta, and differentiate into cells of their own lineage and in some cases atypical lineages. Insight into the stem cell niche leads to the identification of matrix components, soluble factors, and physiological conditions that enhance the ex vivo amplification and differentiation of stem cells. Scaffolds composed of metals, naturally occurring materials, and synthetic polymers organize stem cells into complex spatial groupings that mimic native tissue. Cell signals from covalently bound ligands and slowly released regulatory factors in scaffolds direct stem cell fate. Future advances in stem cell biology and scaffold design will ultimately improve the efficacy of tissue substitutes as implants, in research, and as extracorporeal devices.

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“…This NPC fraction contains a heterogeneous mix of stellate cells, Kupffer cells and biliary cells in addition to the HPC population [11]. Other investigators demonstrated Sca-1 expression on fetal liver stem cell or select oval cell populations generated in specific injury models [17,18,24]. We analyzed the NPC fraction by flow cytometric analysis and found that 15.4% ± 3.7% of the cells in this fraction express Sca-1.…”

Section: Enrichment and Expansion Of The Sca-1 + Hpc (Sca-hpc)mentioning

confidence: 91%