Long-term exposure to Zn-spiked sewage sludge alters soil community structure

Macdonald, Catriona A.; Singh, Brajesh K.; Peck, J.A.; Schaik, Andrew P. van; Hunter, Louise C.; Horswell, Jacqui; Campbell, Colin D.; Speir, T. W.

doi:10.1016/j.soilbio.2007.04.028

Cited by 33 publications

(26 citation statements)

References 50 publications

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“…DNA was precipitated as described by Macdonald et al (2007), and re-suspended in 30 µL nuclease free water. A Multiplex PCR (Singh et al 2006) was carried out with primers specific for bacteria, fungi and Archaea (Table 1).…”

Section: Microbiological Analysismentioning

confidence: 99%

“…A Multiplex PCR (Singh et al 2006) was carried out with primers specific for bacteria, fungi and Archaea (Table 1). PCR amplification and cycling parameters was carried out as described by Macdonald et al (2007). The bacterial and archaeal primers were used at a concentration of 20 pmol µl −1 and the fungal primers at 40 pmol µL −1 .…”

Section: Microbiological Analysismentioning

confidence: 99%

“…PCR products were cleaned using a ChargeSwitch ® Kit (CS1200010, Invitrogen, UK) according to the manufacturer's instructions. Amplicon digestion, TRF detection and M-TRFLP profile data analysis was carried out as described by Macdonald et al (2007).…”

Section: Microbiological Analysismentioning

confidence: 99%

See 2 more Smart Citations

Is vegetation composition or soil chemistry the best predictor of the soil microbial community?

Mitchell¹,

Hester²,

Campbell

et al. 2010

Plant Soil

117

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Section: Microbiological Analysismentioning

confidence: 99%

Section: Microbiological Analysismentioning

confidence: 99%

See 1 more Smart Citation

Is vegetation composition or soil chemistry the best predictor of the soil microbial community?

Mitchell¹,

Hester²,

Campbell

et al. 2010

Plant Soil

117

View full text Add to dashboard Cite

show abstract

“…The soil C: N ratio has been found to be a good predictor of the SMC, with fungal biomass being high when there is a high C:N ratio, and bacterial biomass increasing as this ratio declines (Högberg et al 2007). Heavy metal contamination of the soil also causes changes in the SMC (Frostegård et al 1993;Macdonald et al 2007). Specific communities within the SMC will have different physiological responses to factors such as soil pH, moisture and high metal concentrations, for example many fungi can tolerate lower moisture contents due to their ability to transport water along their hyphal network.…”

mentioning

confidence: 99%

Explaining the variation in the soil microbial community: do vegetation composition and soil chemistry explain the same or different parts of the microbial variation?

et al. 2011

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“…In particular, zinc did not appear to have any effect on microbial biomass nor metabolic activity. Macdonald et al (2007) investigated the effects of Zn-spiked sewage sludge additions on the microbial community structure and microbial after 8 years of exposure to elevated metals. They also found that despite notable changes in community structure, there was no significant long-term impact of Znspiked sludge applications on microbial biomass.…”

Section: Effects Of Copper and Zincmentioning

confidence: 99%

Effect of copper and zinc on microbial tolerance to triclosan in two soil types

et al. 2016

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Purpose Sewage sludge and biosolid application to land is a common approach to fertilise soils, but sewage-derived contaminants like the antimicrobial agent triclosan, and heavy metals zinc and copper, are known to affect soil microbial communities. In this study, the tolerance to triclosan was examined for soil microbial communities chronically preexposed to one of two heavy metals (Cu or Zn) and the antimicrobial triclosan. This was investigated in two different soil types. Materials and methods The impacts of chronic exposure of copper, zinc and triclosan as individual compounds or in mixtures on soil microbial communities were assessed in soils collected from two sites. The first was a Horotiu sandy loam with ample carbon and nitrogen levels and the second was a Templeton silt loam with very low carbon and nitrogen levels. The end points used to characterise the response of the soil microbial community were biomass, metabolic activity and pollution-induced community tolerance (PICT) to triclosan (using Biolog EcoPlates). In addition, metabolic activities for individual substrates were examined and those that significantly changed with the applied treatments were identified. Results and discussion Exposure to mixtures of both triclosan and copper in the Horotiu sandy loam reduced microbial biomass, increased metabolic activity and reduced microbial tolerance to triclosan. The decrease in soil microbial tolerance correlated with an increased metabolic activity for N-acetyl-Dglucosamine providing a potential link between triclosan exposure and nitrogen mineralisation. Exposure to both triclosan and high zinc levels decreased microbial biomass in the Horotiu sandy loam but did not have an effect on microbial tolerance to triclosan. In the finer-textured and less fertile Templeton soil, microbial tolerance to triclosan and the microbial biomass were not impacted by copper/triclosan or zinc/triclosan mixtures. Conclusions Mixture effects could become a cause for concern when soil microbial communities are exposed to triclosan in fertile soils with copper concentrations in excess of 50 mg kg −1 and could be especially important in the more coarsely textured soils. Current regulations for soil contaminants only consider the risk and effects of single contaminants. Greater protection of soil resources could result from considering mixture effects and soil types.

show abstract

Long-term exposure to Zn-spiked sewage sludge alters soil community structure

Cited by 33 publications

References 50 publications

Is vegetation composition or soil chemistry the best predictor of the soil microbial community?

Is vegetation composition or soil chemistry the best predictor of the soil microbial community?

Explaining the variation in the soil microbial community: do vegetation composition and soil chemistry explain the same or different parts of the microbial variation?

Effect of copper and zinc on microbial tolerance to triclosan in two soil types

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