Long-Term Exposure to Isoflavones Alters the Hormonal Steroid Homeostasis-Impairing Reproductive Function in Adult Male Wistar Rats

Cáceres, Sara; Crespo, Belén; Alonso-Diez, Ángela; Andrés, Paloma Jimena de; Millán, P.; Silván, Gema; Illera, M.; Illera, Juan Carlos

doi:10.3390/nu15051261

Cited by 5 publications

(11 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, an imbalance in androgen or estrogen production can affect spermatogenesis [ 4 , 11 ]. Recent findings defend flavonoids and isoflavones such as chrysin, apigenin, luteolin, quercetin, and daidzein along with genistein, which may be effective in delaying the initiation of late-onset hypogonadism associated with aging in males to improve testosterone production, contributing to normal spermatogenesis, male fertility, and preventing age-related degenerative diseases associated with testosterone deficiency [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…Decreases in the testis weight of adult male rats exposed to mixtures of isoflavones were previously reported. Rats receiving genistein + daidzein (17 mg/kg/day and 12 mg/kg/day, respectively) or higher doses (170 mg mg/kg/day + 120 mg/kg/day, respectively) presented decreased testis relative weight after 12 weeks [ 11 ]. In our study, testicle changes were observed after such a short exposure time.…”

Section: Discussionmentioning

confidence: 99%

“…In our study, testicle changes were observed after such a short exposure time. Changes in sex steroids hormones and sperm quality can occur in the testicles due to variations in the size and morphological cellular alterations [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

“…These receptors are present with specific selectivity in several estradiol-responsive tissues, including those that are part of the hypothalamic–pituitary–gonadal axis, responsible for the reproductive function [ 4 ]. Additionally, the effects of genistein appear to be dose-dependent and vary by individual [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of Genistein as a Mitochondrial Modulator and Its Effects on Sperm Quality

Ferigolo,

Nardi,

Freddo

et al. 2023

IJMS

View full text Add to dashboard Cite

Phytoestrogens, such as isoflavones, are bioactive compounds found in plants with defense and protection functions. In the human body, they simulate the behavior of the hormone estradiol and can modulate the function of the male hypothalamic–pituitary–gonadal axis. This study aims to describe the effects of genistein on sperm quality of Wistar rats (male/adult) after a short oral administration protocol (50 mg/day, for 5 days), focusing on mitochondrial function. No signs of toxicity were observed in the animals during the period. The testicular mass of rats from the genistein-treated group was lower than that from the control group. Isoflavone increased the number of viable Leydig and Sertoli cells, spermatogonia, and primary spermatocytes in the treated group. The rounded spermatid count was similar to the control group, and a decrease in elongated spermatids was observed in the treated group. Genistein treatment increased plasma testosterone levels in the treated group. To the best of our knowledge, this is the first report of an in vivo short protocol demonstrating that genistein administration stimulates the overall oxygen consumption in rat seminal samples. Therefore, genistein induced a pro-spermatogenesis effect, enhanced plasma testosterone levels, and increased oxygen consumption, improving sperm mitochondrial efficiency. Similar protocols can be explored in animal and human infertility issues.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of Genistein as a Mitochondrial Modulator and Its Effects on Sperm Quality

Ferigolo,

Nardi,

Freddo

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…), propose that soybean-based meat alternatives should not be considered ultra-processed foods [202] and that soybean isoflavones should not be classified as endocrine disruptors [203]. The extent to which the pre-clinical endocrine-disrupting effects of soy isoflavones (and their gut microbiome metabolites) extend to humans remains an open question [204]. The extent to which the soy industry is shaping international discourse is also an open question [85,205,206].…”

Section: Dietary Displacement Planetary Healthmentioning

confidence: 99%

Beyond Plants: The Ultra-Processing of Global Diets Is Harming the Health of People, Places, and Planet

Prescott

D’Adamo

Holton

et al. 2023

IJERPH

View full text Add to dashboard Cite

Global food systems are a central issue for personal and planetary health in the Anthropocene. One aspect of major concern is the dramatic global spread of ultra-processed convenience foods in the last 75 years, which is linked with the rising human burden of disease and growing sustainability and environmental health challenges. However, there are also calls to radically transform global food systems, from animal to plant-derived protein sources, which may have unintended consequences. Commercial entities have moved toward this “great plant transition” with vigor. Whether motivated by profit or genuine environmental concern, this effort has facilitated the emergence of novel ultra-processed “plant-based” commercial products devoid of nutrients and fiber, and sometimes inclusive of high sugar, industrial fats, and synthetic additives. These and other ingredients combined into “plant-based” foods are often assumed to be healthy and lower in calorie content. However, the available evidence indicates that many of these products can potentially compromise health at all scales—of people, places, and planet. In this viewpoint, we summarize and reflect on the evidence and discussions presented at the Nova Network planetary health meeting on the “Future of Food”, which had a particular focus on the encroachment of ultra-processed foods into the global food supply, including the plant-sourced animal protein alternatives (and the collective of ingredients therein) that are finding their way into global fast-food chains. We contend that while there has been much uncritical media attention given to the environmental impact of protein and macronutrient sources—meat vs. novel soy/pea protein burgers, etc.—the impact of the heavy industrial processing on both human and environmental health is significant but often overlooked, including effects on cognition and mental health. This calls for a more nuanced discourse that considers these complexities and refocuses priorities and value systems towards mutualistic solutions, with co-benefits for individuals, local communities, and global ecology.

show abstract

Effect of Repeated Exposure of Soy Flour on Testicular Histology and Morphology of Mice Spermatozoa

Primiani,

Waris,

Susanto

et al. 2023

EJBIO

View full text Add to dashboard Cite

Isoflavones are active compounds in soybean seeds with a chemical structure resembling 17 β-estradiol and are able to bind to estrogen receptors on Leydig cells. The chemical structure and physiological activity of isoflavones are similar to estrogen. This research was conducted to evaluate changes in the structure of testicular seminiferous tubule tissue and spermatozoa morphology. Male Balb/c mice, 9 weeks old, 20–25 g, 24 mice, grouped into 4 treatments: 6 mice in control group (P0), 6 mice given 0.369 g/kg soy flour (P1), 6 mice given 0.369 g/kg soy flour 0.74 g/kg (P2), 6 mice given soybean flour 1.47 g/kg (P3) treatment for 36 days. Surgery and removal of testicular organs were carried out on the 37th day, and HE staining and preparation of spermatozoa smears were made. Data analysis parameters are changes in the structure of the testicular seminiferous tubule tissue and testicular germ cells (spermatogonia, primary spermatocytes, secondary spermatocytes, spermatids, and spermatozoa). Spermatozoa abnormalities include the head, neck, and tail. The results showed that the administration of soy flour caused the widening of the testicular tubule lumen; no spermatozoa were found in the tubule lumen. Spermatozoa abnormalities in treatment P1 was 18%, P2 was 29%, and P3 was 38%.

show abstract

Long-Term Exposure to Isoflavones Alters the Hormonal Steroid Homeostasis-Impairing Reproductive Function in Adult Male Wistar Rats

Cited by 5 publications

References 40 publications

Evaluation of Genistein as a Mitochondrial Modulator and Its Effects on Sperm Quality

Evaluation of Genistein as a Mitochondrial Modulator and Its Effects on Sperm Quality

Beyond Plants: The Ultra-Processing of Global Diets Is Harming the Health of People, Places, and Planet

Effect of Repeated Exposure of Soy Flour on Testicular Histology and Morphology of Mice Spermatozoa

Contact Info

Product

Resources

About