Long-term exposure of the male mouse to lead: Effects on fertility

Johansson, Lars; Wide, Mariann

doi:10.1016/s0013-9351(86)80142-6

Cited by 52 publications

(16 citation statements)

References 11 publications

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“…1993). These results were similar to those achieved by other authors (Johansson and Wide 1986;Quintanilla-Vega et al 2000). In our current study of rats subjected to chronic low-dose lead intoxication for nine months (duration of five spermatogeneses), the lead concentration in whole blood was significantly higher (7.03 lg/dL) than in the blood of rats from the control group (0.76 lg/dL).…”

Section: Discussionsupporting

confidence: 93%

Disturbances of energetic metabolism in rat epididymal epithelial cells as a consequence of chronic lead intoxication

et al. 2009

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Adult male Wistar rats were intoxicated with 1% lead acetate (PbAc) administered in drinking water for nine months, which amounts to a period five times longer than the duration of one spermatogenesis. There were mitochondrial ultrastructure disorders of epididymal epithelial cells observed in PbAc-treated rats; also a significant lead-induced decrease in ATP concentration in epididymal epithelial cells (by 32%, P < 0.05), Adenylate Energy Charge value (AEC) (by 8%, P < 0.05) and an increase in ADP (28.5%, P < 0.05), AMP (27%, P < 0.05) and adenosine (by 56%, P < 0.05). The results were measured using high performance liquid chromatography (HPLC) and detected even at low lead concentrations in whole blood (M:7.03 μg/dL; Q1-Q3: 2.99-7.65). The function of mitochondria in cultured epididymal epithelial cells of control and PbAc-treated animals were evaluated using fluorophores: Mitotracker Green FM and JC-1. After incubation with Mitotracker Green FM, we observed active mitochondria producing bright green fluorescence in the cytoplasm of cultured epididymal epithelial cells, both in the control group and the Pb-treated animals. Incubation of cultured epididymal epithelial cells of animals from both groups produced red-orange fluorescence with the mitochondrial JC-1 probe indicating mitochondria with high membrane potential (ΔΨm > 80-100 mV) and green fluorescence in the mitochondria with low membrane potential (ΔΨm < 80 mV). The results showed that a chronic low-level exposure to lead, even without severe clinical symptoms of contamination, disrupted the ultrastructure and energy metabolism of mitochondria in epididymal epithelial cells.

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Section: Discussionsupporting

confidence: 93%

Disturbances of energetic metabolism in rat epididymal epithelial cells as a consequence of chronic lead intoxication

et al. 2009

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“…Also, individuals residing near a metallurgic zone with low sperm motility have shown higher seminal plasma levels of lead than a control group [Mora Ân-Martõ Ânez, 1998]. In addition, animal studies have shown that lead levels in male reproductive organs are among the highest after long-term exposures to the metal [Johansson and Wide, 1986;Batra et al, 1998].…”

Section: Discussionmentioning

confidence: 99%

Lead effects on protamine-DNA binding

et al. 2000

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“…Although suspected for a long time [Uzych, 1985], the possibility that lead could also influence male reproductive health was further fueled by observations published by Lancranjan et al [1975]. Subsequent cross-sectional studies have also reported that decrements of semen quality may be present in groups of men with occupational lead exposure [Wildt et al, 1983;Assennato et al, 1986;Lerda, 1992;Alexander et al, 1996;Kuo et al, 1997].Experimental studies support the notion that lead exposure in males causes decreased fertility [Johansson and Wide, 1986] and also results in lowered intratesticular sperm counts, decreased sperm production rates, and subnormal serum-testosterone [Sokol, 1989]. Clastogenic [Grandjean et al, 1983], hormonal [Braunstein et al, 1978;Rodamilans et al, 1988;McGregor and Mason, 1990;Ng et al, 1991], and neurotoxic [Grandjean, 1978] effects of lead have also been demonstrated in humans and could conceivably be involved in adverse effects on sperm quality.…”

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confidence: 99%

Improvement in semen quality associated with decreasing occupational lead exposure

Viskum

Rabjerg

Jørgensen

et al. 1999

American J Industrial Med

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Background Cross‐sectional studies have suggested that occupational lead exposure may adversely affect sperm quality. >Methods Sperm quality changes were prospectively assessed in 19 men employed at a car battery plant where efforts were made to decrease the exposure level. The participants delivered monthly samples of semen and venous blood during their employment at the factory. The factory then closed, and additional samples were obtained from 16 of the men. Results Average blood‐lead concentrations decreased from 2.03 >mol/l to 0.96 >mol/l during the observation period. Concomitantly, significant improvements were seen in the proportion of motile cells both at sample delivery and after 24 hr, and in penetration. However, the sperm cell concentration and the proportion of morphological abnormalities did not change. Conclusions These results support the notion that occupational lead exposure at currently acceptable levels has a small adverse effect on sperm quality, especially sperm motility, and that this effect is at least partially reversible. Am. J. Ind. Med. 35:257–263, 1999. © 1999 Wiley‐Liss, Inc.

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Long-term exposure of the male mouse to lead: Effects on fertility

Cited by 52 publications

References 11 publications

Disturbances of energetic metabolism in rat epididymal epithelial cells as a consequence of chronic lead intoxication

Disturbances of energetic metabolism in rat epididymal epithelial cells as a consequence of chronic lead intoxication

Lead effects on protamine-DNA binding

Improvement in semen quality associated with decreasing occupational lead exposure

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