Long-term estradiol-17β administration reduces population of neurons in the sympathetic chain ganglia supplying the ovary in adult gilts

Koszykowska, Marlena; Całka, Jarosław; Gańko, Marta; Jana, Barbara

doi:10.1016/j.yexmp.2011.04.002

Cited by 21 publications

(21 citation statements)

References 43 publications

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“…A decrease in the total number of ovarian perikarya, including small ones, was revealed earlier in the CaMG of long‐term E 2 ‐treated gilts . In addition, E 2 led to a reduction in the total populations of ovarian perikarya in SChGs , DRGs and PCG . We assume that the E 2 level increase noted in the present study might be accompanied by down‐regulated oestrogen receptors (ERs) expression in the small perikarya.…”

Section: Discussionsupporting

confidence: 61%

“…Indeed, the pig, as a result of its embryological, anatomical and physiological similarities to humans, comprises an especially valuable species for biomedical research, including that of the ovary functions . Our earlier studies revealed that long‐term 17β‐oestradiol (E 2 ) administration changed the specific morphological and immunochemical structural organisation of the CaMG , sympathetic chain ganglia (SChGs) , DRGs and the paracervical ganglion (PCG) ovary‐projecting neurones (referred to further as ovarian perikarya or ovarian neurones) in adult gilts. Based on these findings, we hypothesise that elevated levels of endogenous androgens during pathological states can affect the morphological and chemical plasticity of sympathetic ovarian neurones in the CaMG and, as a result, change gonadal functions.…”

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confidence: 99%

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Reduction of the Number of Neurones in the Caudal Mesenteric Ganglion Innervating the Ovary in Sexually Mature Gilts Following Testosterone Administration

Jana

Rytel

Czarzasta

et al. 2013

J Neuroendocrinology

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The effect of testosterone on the morphological and chemical plasticity of the porcine caudal mesenteric ganglion (CaMG) ovary‐projecting neurones was investigated. To identify the neurones on day 3 of the oestrous cycle, the ovaries of both the control and experimental gilts were injected with Fast Blue retrograde neuronal tracer. From next day until day 20 of the anticipated second studied cycle, experimental gilts were injected with testosterone, whereas control gilts received oil. Testosterone injections increased testosterone (by approximately 3.5‐fold) and 17β‐oestradiol (by approximately 1.6‐fold) levels in the peripheral blood and decreased the following in the CaMG: the total number of Fast Blue‐positive perikarya (including small ones); the population of small perikarya in the caudal, ventral and dorsal ganglional regions; the numbers of dopamine‐β‐hydroxylase (DβH) and/or neuropeptide Y (NPY), somatostatin (SOM), galanin (GAL) small and large perikarya; the numbers of small perikarya containing DβH (but not NPY, SOM, GAL); and the density of DβH and/or NPY, SOM nerve fibres. A disappearance of small and large non‐noradrenergic perikarya and an increase in the total number of androgen receptor‐immunoreactive perikarya was noted. Our results suggest that elevated androgen levels occurring during pathological states may regulate ovary function(s) by affecting the CaMG gonad‐supplying neurones.

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Section: Discussionsupporting

confidence: 61%

mentioning

confidence: 99%

Reduction of the Number of Neurones in the Caudal Mesenteric Ganglion Innervating the Ovary in Sexually Mature Gilts Following Testosterone Administration

Jana

Rytel

Czarzasta

et al. 2013

J Neuroendocrinology

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“…86-23, revised 1985). As the present study was designated to provide basic data concerning the chemical phenotypes of IMG neurons both under physio- as well as patophysiological conditions (axotomy), the authors decided to focus on sexually immature female animals in order to exclude any possible influences of reproductive hormones on studied cells as identified in previous studies (Purves-Tyson and Keast 2004; Koszykowska et al 2011). …”

Section: Methodsmentioning

confidence: 99%

Axotomy-Induced Changes in the Chemical Coding Pattern of Colon Projecting Calbindin-Positive Neurons in the Inferior Mesenteric Ganglia of the Pig

et al. 2013

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The present study examines the response of colon-projecting neurons localized in the inferior mesenteric ganglia (IMG) to axotomy in the pig animal model. In all animals (n = 8), a median laparotomy was performed under anesthesia and the retrograde tracer Fast Blue was injected into the descending colon wall. In experimental animals (n = 4), the descending colon was exposed and the bilateral caudal colonic nerves were identified and severed. All animals were euthanized and the inferior mesenteric ganglia were harvested and processed for double-labeling immunofluorescence for calbindin-D28k (CB) in combination with either tyrosine hydroxylase (TH), neuropeptide Y (NPY), somatostatin (SOM), vasoactive intestinal polypeptide (VIP), nitric oxide synthase (NOS), Leu-enkephalin (LENK), substance P, vesicular acetylcholine transporter, or galanin. Immunohistochemistry revealed significant changes in the chemical coding pattern of injured inferior mesenteric ganglion neurons. In control animals, Fast Blue-positive neurons were immunoreactive to TH, NPY, SOM, VIP, NOS, LENK, and CB. In the experimental group, the numbers of TH-, NPY-, and SOM-expressing neurons were reduced, whereas the number of neurons immunoreactive to LENK was increased. Our data indicate that the colon-projecting neurons of the porcine IMG react to the axotomy in a similar, but not an identical manner in a comparison to other species, especially rodents. Further studies are needed to elucidate the detailed factors/mechanisms involved in the response to nerve injury.

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“…On the other hand, the changed chemical coding of neurons may be a result of the direct effects of steroid hormones on these cells as well. It is recognized that estrogen receptors are expressed in the ovarian neurons of sympathetic and parasympathetic as well as sensory ganglia in adult gilts and that the long-term 17β-estradiol treatment reduced the population of these neurons (Koszykowska et al 2011a, b; Jana et al 2012, 2013a). A similar situation has been revealed for neurons in the caudal mesenteric ganglion innervating the ovary in adult gilts following testosterone administration (Jana et al 2013b).…”

Section: Discussionmentioning

confidence: 99%

Changes in the Cholinergic Innervation Pattern of Porcine Ovaries with Cysts Induced by Dexamethasone Administration

2014

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We revealed earlier that induction of ovarian cysts in gilts by dexamethasone phosphate disodium salt (DXM) administration from the follicular phase of the estrous cycle (EC) changed the cholinergic innervation of the gonad. In the present study, the innervation of porcine ovaries by vesicular acetylcholine transporter (VAChT)-, neuronal nitric oxide synthase (nNOS)-, vasoactive intestinal peptide (VIP)- and somatostatin (SOM)-immunoreactive (IR) fibres, after induction of cystic changes from the middle luteal phase of the EC, was determined. The cystic changes were induced by DXM injections from days 7 to 21 of the EC, and 11 days later, the ovaries were collected. In the cystic ovaries, VAChT-, nNOS- and SOM-IR fibres were found around cysts and small tertiary follicles; nNOS-IR and also VAChT-IR fibres were observed near secondary follicles and veins; and VAChT- and nNOS-IR fibres were not found around cortical arteries. The number of VIP-IR fibres increased near the cysts and within the ground plexus, while the number of VAChT-IR fibres decreased within the medullar part of this structure. Thus, our study showed changes in the cholinergic innervation pattern of the porcine cystic ovaries induced from the middle phase of the cycle and confirmed that cystic ovary innervation depends partly on the phase of the EC in which the induction of cysts was started.

show abstract

Long-term estradiol-17β administration reduces population of neurons in the sympathetic chain ganglia supplying the ovary in adult gilts

Cited by 21 publications

References 43 publications

Reduction of the Number of Neurones in the Caudal Mesenteric Ganglion Innervating the Ovary in Sexually Mature Gilts Following Testosterone Administration

Reduction of the Number of Neurones in the Caudal Mesenteric Ganglion Innervating the Ovary in Sexually Mature Gilts Following Testosterone Administration

Axotomy-Induced Changes in the Chemical Coding Pattern of Colon Projecting Calbindin-Positive Neurons in the Inferior Mesenteric Ganglia of the Pig

Changes in the Cholinergic Innervation Pattern of Porcine Ovaries with Cysts Induced by Dexamethasone Administration

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