Long-term effects of three compound probiotics on water quality, growth performances, microbiota distributions and resistance to Aeromonas veronii in crucian carp Carassius auratus gibelio

Zhang, Yingying; Ji, Tongwei; Jiang, Yifeng; Zheng, Chen; Yang, Hui; Liu, Qiu-Ning

doi:10.1016/j.fsi.2021.11.036

Cited by 24 publications

(10 citation statements)

References 40 publications

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“…Increasing research has shown that probiotics applied for regulation can effectively remove nutrients from water, especially nitrogen, and inhibit eutrophication [ 39 , 40 , 41 ]. In crucian carp ( Carassius auratus gibelio ), exposure to high concentrations of complex probiotics resulted in the attainment of a dynamic equilibrium state for total ammonia nitrogen, and nitrite levels [ 42 ]. The mixed probiotics have shown positive effects on reducing nitrogen and phosphorus compounds [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Metagenomic Insight into the Effect of Probiotics on Nitrogen Cycle in the Coilia nasus Aquaculture Pond Water

Mang,

Gao,

et al. 2024

Microorganisms

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Recently, probiotics have been widely applied for the in situ remediation of aquatic water. Numerous studies have proved that probiotics can regulate water quality by improving the microbial community. Nitrogen cycling, induced by microorganisms, is a crucial process for maintaining the balance of the aquatic ecosystem. Nevertheless, the underlying mechanisms by which probiotics enhance water quality in aquatic systems remain poorly understood. To explore the water quality indicators and their correlation with nitrogen cycling-related functional genes, metagenomic analysis of element cycling was performed to identify nitrogen cycling-related functional genes in Coilia nasus aquatic water between the control group (C) and the groups supplemented with probiotics in feed (PF) or water (PW). The results showed that adding probiotics to the aquatic water could reduce the concentrations of ammonia nitrogen (NH4+-N), nitrite (NO2−-N), and total nitrogen (TN) in the water. Community structure analysis revealed that the relative abundance of Verrucomicrobiota was increased from 30 d to 120 d (2.61% to 6.35%) in the PW group, while the relative abundance of Cyanobacteria was decreased from 30 d to 120 d (5.66% to 1.77%). We constructed a nitrogen cycling pathway diagram for C. nasus aquaculture ponds. The nitrogen cycle functional analysis showed that adding probiotics to the water could increase the relative abundance of the amoC_B and hao (Nitrification pathways) and the nirS and nosZ (Denitrification pathways). Correlation analysis revealed that NH4+-N was significantly negatively correlated with Limnohabitans, Sediminibacterium, and Algoriphagus, while NO2−-N was significantly negatively correlated with Roseomonas and Rubrivivax. Our study demonstrated that adding probiotics to the water can promote nitrogen element conversion and migration, facilitate nitrogen cycling, benefit ecological environment protection, and remove nitrogen-containing compounds in aquaculture systems by altering the relative abundance of nitrogen cycling-related functional genes and microorganisms.

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Section: Discussionmentioning

confidence: 99%

Metagenomic Insight into the Effect of Probiotics on Nitrogen Cycle in the Coilia nasus Aquaculture Pond Water

Mang,

Gao,

et al. 2024

Microorganisms

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“…Malondialdehyde (MDA) is negatively correlated with antioxidant capacity, so it is useful to evaluate antioxidant capacity in fish [42]. Studies have shown that SOD and CAT in intestine of crucian carp increased significantly after feeding complex probiotics (10 6 -10 8 cfu/ml) [43]. In this study, SOD and T-AOC levels were higher in group B than in the other groups, and groups BE and E also increased but not significantly compared to the control group.…”

Section: Discussionmentioning

confidence: 99%

Effects of Probiotics and Its Extracellular Products on the Growth Performance, Immune Response, and Aeromonas hydrophila Resistance of Grass Carp

Gao,

Peng,

Gao

et al. 2024

Aquaculture Research

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The goal of this study was to research the effects of Lactobacillus buchneri L3-9 and its extracellular products on growth, immunity, intestinal microorganisms, and disease resistance to Aeromonas hydrophila in grass carp. A total of 120 fish (80–90 g) were distributed into 12 aquariums (10 fish/aquarium) in three replicates and fed 1% of their body weight for 21 days. The grass carp were fed four different diets: a control (group C), a diet supplemented with 1 × 106 cell g−1 of L. buchneri L3-9 (group B), a diet supplemented with extracellular products of L. buchneri L3-9 (group E), and a diet supplemented with L. buchneri L3-9 and the extracellular products (group BE). Compared with the control diet, the B, E, and BE diets increased the weight gain rate by 4.49%, 2.59%, and 4.38%, respectively (p <0.05), and significantly decreased the feed conversion ratio by 0.72, 0.57, and 0.76, respectively (p<0.05). Groups B, BE, and E showed significant decreases in mortality, with values of 26.67%, 16.67%, and 23.33%, respectively (p<0.05). Group B showed a significant increase in superoxide dismutase (SOD) activity and total antioxidant capacity (T-AOC) and decrease in malondialdehyde (MDA) content compared to the control group (p<0.05). The highest catalase (CAT) activity was found in group E (p<0.05). In group B, the expression of the gut proinflammatory gene TNF-α was downregulated (p<0.05), and the expression of anti-inflammatory genes, including TGF-β1, IL-10, and Tlr-8, was upregulated compared to that in the control group (p<0.05). Groups BE and E showed an increased abundance of Lactobacillaceae. Additionally, a drastic decrease in the abundance of pathogenic bacteria such as Aeromonadaceae and Enterobacteriaceae was in these groups compared to the control group. In conclusion, L. buchneri L3-9 and its extracellular products could improve the growth performance, immune responses, and resistance to A. hydrophila in grass carp. This study provides insights for the development and application of microecological preparations.

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“…It has been proven that the activities of antioxidant enzymes can be regarded as indicators of oxidative stress ( Wang et al, 2013 ). Various antioxidant enzymes and non-enzymatic antioxidants can effectively remove excess reactive oxygen species (ROS) and protect against oxidative damage ( Zhang et al, 2022 ). However, when the stress exceeds the tolerance, the scavenging capacity of the antioxidant defense system is decreased ( Winston, 1991 ; Mathew et al, 2007 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of stocking density on the growth performance, mitophagy, endocytosis and metabolism of Cherax quadricarinatus in integrated rice–crayfish farming systems

et al. 2022

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Red claw crayfish (Cherax quadricarinatus) is an economic freshwater shrimp with great commercial potential. However, the suitable stocking density of C. quadricarinatus is still unclear in integrated rice–crayfish farming system. Thus, this study aimed to investigate the effects of stocking density on growth performance, mitophagy, endocytosis and metabolism of C. quadricarinatus. The C. quadricarinatus was reared at low density (LD, 35.73 g/m2), middle density (MD, 71.46 g/m2) and high density (HD, 107.19 g/m2) in an integrated rice–crayfish farming system. After 90 days of farming, the growth performance of C. quadricarinatus significantly decreased in the MD and HD groups relative to that in the LD group. The HD treatment caused oxidative stress and lipid peroxidation at the end of the experiment in hepatopancreas. Transcriptome analysis showed that there were 1,531 DEGs (differently expressed genes) between the LD group and HD group, including 1,028 upregulated genes and 503 downregulated genes. KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis indicated that the DEGs were significantly enriched in endocytosis and mitophagy pathways. Meanwhile, four lipid metabolism pathways, including biosynthesis of unsaturated fatty acids, fatty acid biosynthesis, glycerolipid metabolism and glycerophospholipid metabolism, exhibited an upregulated tendency in the HD group. In conclusion, our data showed that when the stocking density reached up to 207.15 g/m2 in HD group, the growth performance of C. quadricarinatus was significantly inhibited in this system. Meanwhile, the data indicated that C. quadricarinatus may respond to the stressful condition via activating antioxidant defense system, endocytosis, mitophagy and metabolism-related pathways in hepatopancreas.

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Long-term effects of three compound probiotics on water quality, growth performances, microbiota distributions and resistance to Aeromonas veronii in crucian carp Carassius auratus gibelio

Cited by 24 publications

References 40 publications

Metagenomic Insight into the Effect of Probiotics on Nitrogen Cycle in the Coilia nasus Aquaculture Pond Water

Metagenomic Insight into the Effect of Probiotics on Nitrogen Cycle in the Coilia nasus Aquaculture Pond Water

Effects of Probiotics and Its Extracellular Products on the Growth Performance, Immune Response, and Aeromonas hydrophila Resistance of Grass Carp

Effects of stocking density on the growth performance, mitophagy, endocytosis and metabolism of Cherax quadricarinatus in integrated rice–crayfish farming systems

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