Long-term effects of hydrogel properties on human chondrocyte behavior

Klein, Travis J.; Rizzi, Simone C.; Schrobback, Karsten; Reichert, Johannes C.; Jeon, June; Crawford, Ross; Hutmacher, Dietmar W.

doi:10.1039/c0sm00229a

Cited by 51 publications

(54 citation statements)

References 55 publications

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“…When combined with growth factors such as TGF-b1, hydrogels such as alginate [8] and agarose [9] have been shown to help chondrocytes maintain their spherical morphology while supporting chondrogenic differentiation and cartilage-specific matrix deposition. Cartilage constructs can also be mechanically stimulated in vitro to enhance chondrocyte matrix synthesis and remodelling [10,11], and to recapitulate zonal characteristics in the construct [12,13].…”

Section: Introductionmentioning

confidence: 99%

Multiphasic construct studied in an ectopic osteochondral defect model

Jeon

Vaquette

Theodoropoulos

et al. 2014

J. R. Soc. Interface.

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In vivo osteochondral defect models predominantly consist of small animals, such as rabbits. Although they have an advantage of low cost and manageability, their joints are smaller and more easily healed compared with larger animals or humans. We hypothesized that osteochondral cores from large animals can be implanted subcutaneously in rats to create an ectopic osteochondral defect model for routine and high-throughput screening of multiphasic scaffold designs and/or tissue-engineered constructs (TECs). Bovine osteochondral plugs with 4 mm diameter osteochondral defect were fitted with novel multiphasic osteochondral grafts composed of chondrocyteseeded alginate gels and osteoblast-seeded polycaprolactone scaffolds, prior to being implanted in rats subcutaneously with bone morphogenic protein-7. After 12 weeks of in vivo implantation, histological and micro-computed tomography analyses demonstrated that TECs are susceptible to mineralization. Additionally, there was limited bone formation in the scaffold. These results suggest that the current model requires optimization to facilitate robust bone regeneration and vascular infiltration into the defect site. Taken together, this study provides a proof-of-concept for a high-throughput osteochondral defect model. With further optimization, the presented hybrid in vivo model may address the growing need for a cost-effective way to screen osteochondral repair strategies before moving to large animal preclinical trials.

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Section: Introductionmentioning

confidence: 99%

Multiphasic construct studied in an ectopic osteochondral defect model

Jeon

Vaquette

Theodoropoulos

et al. 2014

J. R. Soc. Interface.

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“…Among them, poly(ethylene glycol) (PEG) hydrogel was most prevalent class of synthetic materials for cartilage tissue engineering. PEG hydrogels are relatively inert and biocompatible, and could support chondrocytes and mesenchymal stem cells growth, but it was limited to support specific cartilage matrix production identical to the natural tissue [13]. Natural hydrogels based on proteins and polysaccharides are also helpful Fengxuan Han and Xiaoling Yang contributed equally to this work.…”

Section: Introductionmentioning

confidence: 97%

Photocrosslinked layered gelatin-chitosan hydrogel with graded compositions for osteochondral defect repair

Han

Yang

Zhao

et al. 2015

J Mater Sci: Mater Med

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A layered gelatin-chitosan hydrogel with graded composition was prepared via photocrosslinking to simulate the polysaccharide/collagen composition of the natural tissue and mimic the multi-layered gradient structure of the cartilage-bone interface tissue. Firstly, gelatin and carboxymethyl chitosan were reacted with glycidyl methacrylate (GMA) to obtain methacrylated gelatin (Gtn-GMA) and carboxymethyl chitosan (CS-GMA). Then, the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA were prepared to form the superficial, transitional and deep layers of the hydrogel, respectively under the irradiation of ultraviolet light, while polyhedral oligomeric silsesquioxane was introduced in the deep layer to improve the mechanical properties. Results suggested that the pore sizes of the superficial, transitional and deep layers of the layered hydrogel were 115 ± 30, 94 ± 34, 51 ± 12 μm, respectively and their porosities were all higher than 80 %. The compressive strengths of them were 165 ± 54, 565 ± 50 and 993 ± 108 kPa, respectively and the strain of the gradient hydrogel decreased along the thickness direction, similar to the natural tissue. The in vitro cytotoxicity results showed that the hydrogel had good cytocompatibility and the in vivo repair results of osteochondral defect demonstrated remarkable recovery by using the gradient gelatin-chitosan hydrogel, especially when the hydrogel loading transforming growth factor-β1. Therefore, it was suggested that the prepared layered gelatin-chitosan hydrogel in this study could be potentially used to promote cartilage-bone interface tissue repair.

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“…Biopolymer hydrogels are proving to be useful threedimensional porous scaffolds for tissue engineering and models to mimic extracellular matrices for studies of cell behaviors (Glowacki and Mizuno 2008;Grinnell and Petroll 2010;Petrie et al 2012). Viscoelastic hydrogels made of naturally occurring polymers such as collagen type-I (Wolf et al 2009), fibrinogen (Yang et al 2007), polysaccharides (Klein et al 2010), and Matrigel TM (Collins et al 2010) are biocompatible and possess unique chemical components that play important roles in cell signaling, mimicking in vivo cellular microenvironment. To mimic the microenvironment, numerous factors such as the mechanical properties, surface chemistry, porosity, and biochemical transportation in the hydrogel must be taken into consideration (Barkan et al 2010;Yang et al 2010;Pathak and Kumar 2011).…”

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confidence: 99%