Long-term effects of blue or red light on ATP and ADP contents in primary barley leaves

Abstract

Abstract. Levels of ATP and ADP were studied in primary leaves of barley (Hordeum vulgare L. cv. Viner) seedlings grown under blue (BL) or red light (RL) of various irradiances. In mature leaf segments, BL stimulated a greater accumulation of adenylates than RL. Transfer of barley seedlings from RL to BL for 48 h caused about a twofold increase in the content of adenylates, probably due to de-novo synthesis of adenine nucleotides. Weak BL was found to stimulate an increase in the adenylate content and a higher irradiance enhanced the stimulatory effect. The adenylate content increased markedly from the base towards the tip of barley leaves grown under BL but not in those grown under RL. However, the adenylate content was higher in the basalmost segment of barley leaves grown under RL, indicating that the action of RL on adenylate content proceeded more rapidly than that of BL. The same conclusion could be drawn from the results of experiments with de-etiolated leaves. A linear relationship was established between the maximum rate of CO 2 fixation and the ATP or ADP content in mature segments of primary barley leaves. The possible involvement of two photoreceptors, phytochrome and cryptochrome, in the long-term light regulation of the total content of adenylates in primary barley leaves is discussed.

Adenylate patterns of autumn‐sown sugar beet differ from spring‐sown sugar beet. Implications for root quality

“…The ratio ATP/ADP is a fundamental regulatory and regulated value. High ATP/ADP values have been found in germinating seeds (Ching and Kronstadt 1972) and lower values in roots (Adams 1970) and green tissues (Bukhov et al. 1995).…”

“…The ratio ATP/ADP is a fundamental regulatory and regulated value. High ATP/ADP values have been found in germinating seeds (Ching and Kronstadt 1972) and lower values in roots (Adams 1970) and green tissues (Bukhov et al 1995). It appears that adenylate ratios of non-green plant cells are stabilized at high values as is the case in other living cells, but these ratios are stabilized at lower values in green cells (Pradet and Raymond 1983).…”

“…Frozen samples (0.3 g of storage roots) were ground in a mortar with 5 ml of 0.6 m HClO 4 (Bukhov et al. 1995) and adenylates extracted for 30 min at room temperature.…”

“…Frozen samples (0.3 g of storage roots) were ground in a mortar with 5 ml of 0.6 M HClO 4 (Bukhov et al 1995) and adenylates extracted for 30 min at room temperature. The acid suspension was adjusted to pH 7.6 with 2 M K 2 CO 3 , and the residue was removed by centrifugation.…”

Different regulation of leaf respiration between Spinacia oleracea, a sun species, and Alocasia odora, a shade species

“…Segments were cut from leaves with a fresh razor blade, frozen in liquid nitrogen, and kept at -84°C until analysis. Adenylates were quantitated by the methods of Bukhov et al (1995) and Kobayashi et al (1979). Adenylates were extracted from a leaf sample with 5 ml of 0.25 M HCIO4.…”