Long-term effect of heavy-metal pollution on diversity of gastrointestinal microbial community of Bufo raddei

Zhang, Wenya; Guo, Rui; Yang, Ying; Ding, Jian; Zhang, Yingmei

doi:10.1016/j.toxlet.2016.07.003

Cited by 77 publications

(43 citation statements)

References 25 publications

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“…S2 and Table 1) using MiSeq sequencing of the microbial 16S showed high richness and diversity, but the microbial richness and diversity in the sewage outfall and dosing sites showed a significant reduction compared to those of the control, especially in the sewage outfall samples. These results indicated that the microbial abundance was restrained and the microbial diversity reduced in the sewage outfall due to the existence of Cd, which was consistent with previous reports 35, 37 .…”

Section: Resultssupporting

confidence: 93%

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Geographic distribution of cadmium and its interaction with the microbial community in the Longjiang River: risk evaluation after a shocking pollution accident

Zhang

Huang

Wang

et al. 2017

Sci Rep

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A shocking Longjiang River cadmium pollution accident occurred in 2012, the effects of which on microbial communities remain unclear. Alkaline precipitation technology was applied for remediation, but concerns rose about the stability of this technology. To understand the geographic distribution of cadmium and its correlation with microbes, in this study, 39 water samples and 39 sludge samples from this river and 2 soil samples from the nearby farmland were collected for chemical and microbial analyses. The Cd concentrations of all water samples were lower than 0.005 mg/L and reached the quality standards for Chinese surface water. A ranking of sludge samples based on Cd contents showed sewage outfall > dosing sites > farmland, all of which were higher than the quality standard for soil. Alkaline precipitation technology was effective for Cd precipitation. Cd was unstable; it was constantly dissolving and being released from the sludge. The Cd content of each phase was mainly influenced by the total Cd content. Over 40,000 effective sequences were detected in each sample, and a total of 59,833 OTUs and 1,273 genera were found using Illumina MiSeq sequencing. Two phyla and 39 genera were notably positively correlated with the Cd distribution, while the cases of 10 phyla and 6 genera were the opposite.

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Section: Resultssupporting

confidence: 93%

“…Physical and chemical indices were determined for all the samples. In addition, 16S rRNA high throughput sequencing technology was applied 23, 35, 36 . The goal of this study is to investigate the stability and geographic distribution of Cd and its effects on the bacterial community composition of the Longjiang River.…”

Section: Introductionmentioning

confidence: 99%

Geographic distribution of cadmium and its interaction with the microbial community in the Longjiang River: risk evaluation after a shocking pollution accident

Zhang

Huang

Wang

et al. 2017

Sci Rep

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“…The fecal microbial 16S rRNA gene sequencing and analysis were investigated as previous studies (Cui et al., ; Zhang et al., ). Five mice were randomly selected from each group and transferred to fresh sterilized cages after 6 weeks of feeding.…”

Section: Methodsmentioning

confidence: 99%

Inulin Ameliorates Alcoholic Liver Disease via Suppressing LPS‐TLR4‐Mψ Axis and Modulating Gut Microbiota in Mice

Yang

Zhang

et al. 2019

Alcoholism Clin & Exp Res

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Background: Alcoholic liver disease (ALD) represents a chronic liver disorder caused by alcohol abuse. Numerous studies have demonstrated that gut microbiota dysbiosis plays a critical role in ALD pathogenesis. Application of prebiotic, probiotic, and dietary supplementation to the modulation of gut microbiota contributes to a novel approach to the management of ALD. Inulin, a natural prebiotic found in plants, can restore gut dysbiosis in ALD. However, the exact mechanism of dietary inulin in ALD remains largely unknown.Methods: Sixty female C57BL/6J mice were randomly divided into 4 groups: pair-fed (PF) group (PF/CON); alcohol-fed (AF) group (AF/CON); PF with inulin (INU) group (PF/INU); and AF with INU group (AF/INU). All mice were fed with isocaloric modified Lieber-DeCarli liquid diets with or without alcohol.Results: After 6 weeks of feeding, mice were euthanized and associated indications were investigated. The results showed that chronic ethanol (EtOH) intake led to the loss of body weights, abnormal levels of transaminases, and inflammatory indicators (lipopolysaccharide [LPS], interleukin [IL]-6, IL-10, tumor necrosis factor-a, IL-17A), while inulin administration ameliorated these effects. To further understand the underlying mechanism, we investigated macrophages (Mws) and gut microbiota in diverse groups. The number of Mws was reduced after dietary inulin treatment in chronic EtOH exposure. Hepatic Toll-like receptor 4 (TLR4 + ) Mws in AF/INU group were lower than AF/CON group. 16S rRNA sequencing and analysis of gut microbiota indicated the reduction of Allobaculum, Lactobacillus, and Lactococcus, as well as the increase of Parasutterella in AF group compared with PF control. Increased Allobaculum, Lactobacillus, and Lactococcus but reduced Parasutterella in AF/INU group were confirmed that dietary inulin rectified gut dysbiosis to attenuate ALD.Conclusions: Dietary inulin ameliorates ALD via suppressing LPS-TLR4-Mw axis and modulating gut microbiota in mice, thus potentially provides theoretical foundation for inulin intervention in the prevention and treatment of ALD.

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“…Not all bacteria, however, can tolerate metals and metalloids. For example, the microbiota of mice can be altered when exposed to lead, cadmium (Breton et al, 2013) or selenium (Kasaikina et al, 2011); human gut microbes change in response to arsenic and lead (Bisanz et al, 2014); and the gut microbial community of the Mongolian toad is affected by a combination of copper, cadmium, zinc and lead (Zhang et al, 2016). In light of these studies, using the microbiome to reduce metalloid toxicity is now starting to be explored, with Coryell et al establishing that the mouse gut community assists in reducing host mortality upon arsenic exposure (Coryell et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

The bumble bee microbiome increases survival of bees exposed to selenate toxicity

Rothman

Leger

Graystock

et al. 2019

Environmental Microbiology

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Summary Bumble bees are important and widespread insect pollinators who face many environmental challenges. For example, bees are exposed to the metalloid selenate when foraging on pollen and nectar from plants growing in contaminated soils. As it has been shown that the microbiome of animals reduces metalloid toxicity, we assayed the ability of the bee microbiome to increase survivorship against selenate challenge. We exposed uninoculated or microbiota‐inoculated Bombus impatiens workers to a field‐realistic dose of 0.75 mg l−1 selenate and found that microbiota‐inoculated bees survive slightly but significantly longer than uninoculated bees. Using 16S rRNA gene sequencing, we found that selenate exposure altered gut microbial community composition and relative abundance of specific core bacteria. We also grew two core bumble bee microbes – Snodgrassella alvi and Lactobacillus bombicola – in selenate‐spiked media and found that these bacteria grew in the tested concentrations of 0.001–10 mg l−1 selenate. Furthermore, the genomes of these microbes harbour genes involved in selenate detoxification. The bumble bee microbiome slightly increases survivorship when the host is exposed to selenate, but the specific mechanisms and colony‐level benefits under natural settings require further study.

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Long-term effect of heavy-metal pollution on diversity of gastrointestinal microbial community of Bufo raddei

Cited by 77 publications

References 25 publications

Geographic distribution of cadmium and its interaction with the microbial community in the Longjiang River: risk evaluation after a shocking pollution accident

Geographic distribution of cadmium and its interaction with the microbial community in the Longjiang River: risk evaluation after a shocking pollution accident

Inulin Ameliorates Alcoholic Liver Disease via Suppressing LPS‐TLR4‐Mψ Axis and Modulating Gut Microbiota in Mice

The bumble bee microbiome increases survival of bees exposed to selenate toxicity

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