Long-term dry preservation of viable mycelia of two mycoherbicidal organisms

Current Advances in the Fusarium Wilt Disease Management in Banana with Emphasis on Biological Control

“…This fungal biocontrol agent has long been recognized as biological agents, for the control of plant disease and for their ability to increase root growth and development, crop productivity, resistance to abiotic stresses, and uptake and use of nutrients. It can be efficiently used as spores (especially, conidia), which are more tolerant to adverse environmental conditions during product formulation and field use, in contrast to their mycelial and chlamydospore forms as microbial propagules (Amsellem et al 1999). However, the presence of a mycelial mass is also a key component for the production of antagonistic metabolites (Benhamou and Chet 1993;Yedidia et al 2000).…”

Bioherbicides for weed control.

“…However, the significance of formulation composition as it relates to storage stability of bioherbicide propagules has been demonstrated (Silman et al, 1993;Connick et al, 1996Connick et al, , 1997Amsellem et al, 1999;Shabana et al, 2003;Müller-Stöver et al, 2004;Friesen et al, 2006). For example, in research on the shelf-life of either conidia or conidia plus mycelium of Fusarium arthrosporioides and F. oxysporum, 'Stabileze' (a mixture of starch, sucrose, corn oil and silica) was found to be superior to alginate bead formulations in preserving the viability of each weed control agent (Amsellem et al, 1999). Shelf-life of C. truncatum spores, formulated in a solid/perlite-cornmeal-agar mixture, at 15°C was longer than in a liquid formulation or a solid/vermiculite mixture (Silman et al, 1993).…”

Mycelia Growth and Spore Yield of Trichoderma harzianum in Batch and Fed-Batch Cultures: Influence of pH and Temperature

“…during product formulation and field use, in contrast to their mycelia and chlamysdospore forms as microbial propagules (Amsellem et al, 1999). However, the presence of mycelia along with conidia would insure presence of various essential metabolites (e.g., antibiotics) for BCA activity (Roberts et al, 2005).…”