Long-term dietary l-arginine supplementation increases endothelial nitric oxide synthase and vasoactive intestinal peptide immunoexpression in rat small intestine

Veličković, Ksenija; Markelić, Milica; Golić, Igor; Otašević, Vesna; Stančić, Ana; Janković, Aleksandra; Vučetić, Milica; Buzadžić, Biljana; Korać, Bato; Korać, Aleksandra

doi:10.1007/s00394-013-0585-8

Cited by 11 publications

(6 citation statements)

References 33 publications

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“…The mRNA and protein expressions from the solvent control group were used as the reference values in a-f. Each value is represented by the mean ± SD of five individuals (n=5). The asterisk indicates that there are significant differences (P<0.05) between the control group and the L group at the same time point and local signal transduction molecule in tissue damage [32]. Under physiological conditions, the sustainability and lowlevel expression of NO contribute to maintaining the integrity of the gastrointestinal mucosa.…”

Section: Discussionmentioning

confidence: 98%

The Role of Nitric Oxide and Oxidative Stress in Intestinal Damage Induced by Selenium Deficiency in Chickens

Yao

Gao

et al. 2014

Biol Trace Elem Res

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Nitric oxide (NO) is an essential messenger molecule and is associated with inflammation and oxidative stress. Although NO has important biological functions in mammals, its role in the mechanism that occurs after intestinal injuries in chickens remains unknown. The objective of the present study was to investigate the real role of NO and oxidative stress in the intestinal injuries of chickens induced by selenium (Se) deficiency. A total 150 chickens were randomly divided into the following two groups: a low-Se group (L group, fed a Se-deficient diet containing 0.020 mg/kg Se) and a control group (C group, fed a commercial diet containing 0.2 mg/kg Se). The activities and mRNA levels of glutathione peroxidase (GSH-Px), the production of glutathione (GSH) and NO, and the protein and mRNA levels of inducible nitric oxide synthase (iNOS) were examined in the intestinal tissues (duodenum, jejunum, and rectum) at 15, 25, 35, 45, and 55 days. Methane dicarboxylic aldehyde (MDA) levels were also detected by assay kits. Then, the morphologies of the tissues were observed under the microscope after hematoxylin and eosin staining (H&E staining). The results showed that Se deficiency induced higher inflammatory damage and MDA levels (P < 0.05), which were accompanied by higher levels of iNOS and NO but lower levels of GSH and GSH-Px (P < 0.05). Our results indicated that Se deficiency induced oxidative damage in the intestinal tracts of chickens and that low levels of GSH-Px and high contents of NO may exert a major role in the injury of the intestinal tract induced by Se deficiency.

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Section: Discussionmentioning

confidence: 98%

The Role of Nitric Oxide and Oxidative Stress in Intestinal Damage Induced by Selenium Deficiency in Chickens

Yao

Gao

et al. 2014

Biol Trace Elem Res

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“…Besides, the vasoactive intestinal peptide (VIP), a peptide hormone synthesized in the gut but also brain, could mediate immune-modulation during CNS inflammation(Gonzalez-Rey et al, 2006). While the direct impact of microbiome on VIP expression has not been identified, dietary intervention is able to increase intestinal VIP, which might hint the role of microbiome(Velickovic et al, 2013). …”

Section: Communication Between Gut Microbiome and The Cnsmentioning

confidence: 99%

The role of microbiome in central nervous system disorders

Wang

Kasper

2014

Brain, Behavior, and Immunity

628

416

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Mammals live in a co-evolutionary association with the plethora of microorganisms that reside at a variety of tissue microenvironments. The microbiome represents the collective genomes of these co-existing microorganisms, which is shaped by host factors such as genetics and nutrients but in turn is able to influence host biology in health and disease. Niche-specific microbiome, prominently the gut microbiome, has the capacity to effect both local and distal sites within the host. The gut microbiome has played a crucial role in the bidirectional gut-brain axis that integrates the gut and central nervous system (CNS) activities, and thus the concept of microbiome-gut-brain axis is emerging. Studies are revealing how diverse forms of neuro-immune and neuro-psychiatric disorders are correlated with or modulated by variations of microbiome, microbiota-derived products and exogenous antibiotics and probiotics. The microbiome poises the peripheral immune homeostasis and predisposes host susceptibility to CNS autoimmune diseases such as multiple sclerosis. Neural, endocrine and metabolic mechanisms are also critical mediators of the microbiome-CNS signaling, which are more involved in neuro-psychiatric disorders such as autism, depression, anxiety, stress. Research on the role of microbiome in CNS disorders deepens our academic knowledge about host-microbiome commensalism in central regulation and in practicality, holds conceivable promise for developing novel prognostic and therapeutic avenues for CNS disorders.

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“…For semi-quantification of UCP1 and noradrenaline immunohistochemical stainings, 12 fields per group were randomly selected at 40× magnifications and analysed by ImageJ software. Data for UCP1 are presented as the reciprocal intensity (Nguyen et al, 2013) and data for noradrenaline expression are presented as positive area (Velickovic et al, 2014).…”

Section: Immunohistochemistrymentioning

confidence: 99%

Effects of long-term sucrose overfeeding on rat brown adipose tissue: a structural and immunohistochemical study

Veličković

Ukropina

Čakić-Milošević

2018

Journal of Experimental Biology

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The aim of this study was to determine the effects of long-term sucrose overfeeding on functional capacity and ultrastructural characteristics of the rat brown adipose tissue (BAT). For the study, 16 male Wistar rats, chow-fed and kept under standard laboratory conditions, were divided into 2 equal groups. The rats from a control group drank tap water, whereas those from a sucrose overfed group were allowed to drink 10% sucrose solution for 21 days. Structural changes of BAT were analysed at the level of light and electron microscopy on routinely prepared tissue sections or using immunohistochemical staining, in combination with stereological methods. Obtained results have shown that the significantly increased energy intake in sucrose overfed rats did not result in a higher gain of body mass compared with controls. The light microscopy analysis revealed that the BAT acquired the appearance of a thermogenically active tissue, with intensified vascularisation, reduced size of brown adipocytes and increased multilocularity. At the ultrastructural level, mitochondria of brown adipocytes became more abundant, enlarged and contained more cristae in comparison to control animals. The immunoexpression of uncoupling protein 1 (UCP1) and noradrenaline, as markers of BAT thermogenic status, was increased, whereas the pattern of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α) was slightly modified. Taken together, the results of this investigation indicated that BAT possesses the ability to increase thermogenic capacity/activity in response to high energy intake and to prevent body mass gain. These findings are particularly relevant in view of recent reports on the existence of functional BAT in adult humans and its potential use to combat obesity.

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Long-term dietary l-arginine supplementation increases endothelial nitric oxide synthase and vasoactive intestinal peptide immunoexpression in rat small intestine

Abstract: Our results demonstrate that long-term dietary L-arginine supplementation modulates NOSs and VIP immunoexpression in rat small intestine with respect to ambient temperature, pointing out the eNOS as a predominant NOS isoform with an immunoexpression pattern similar to VIP.

Cited by 11 publications

References 33 publications

The Role of Nitric Oxide and Oxidative Stress in Intestinal Damage Induced by Selenium Deficiency in Chickens

The Role of Nitric Oxide and Oxidative Stress in Intestinal Damage Induced by Selenium Deficiency in Chickens

The role of microbiome in central nervous system disorders

Effects of long-term sucrose overfeeding on rat brown adipose tissue: a structural and immunohistochemical study

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