Long-term degradation study of hyaluronic acid in aqueous solutions without protection against microorganisms

Simulescu, Vasile; Kalina, Michal; Mondek, Jakub; Pekař, Miloslav

doi:10.1016/j.carbpol.2015.10.101

Cited by 23 publications

(13 citation statements)

References 18 publications

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“…In contrast, the other studied molecular weights of hyaluronan in random coil conformation [ 52 ] have approximately the same entropy contribution. Hyaluronan with a molecular weight of 109 kDa, which has a conformation close to the transition between the rod and random coil forms [ 53 ], appears, from the microcalorimetric point of view, more like a random coil in terms of its interactions with arginine oligomers.…”

Section: Discussionmentioning

confidence: 99%

Hyaluronan-Arginine Interactions—An Ultrasound and ITC Study

Jugl

Pekař

2020

Polymers

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High-resolution ultrasound spectroscopy and isothermal titration calorimetry were used to characterize interactions between hyaluronan and arginine oligomers. The molecular weight of arginine oligomer plays an important role in interactions with hyaluronan. Interactions were observable for arginine oligomers with eight monomer units and longer chains. The effect of the ionic strength and molecular weight of hyaluronan on interactions was tested. In an environment with increased ionic strength, the length of the arginine oligomer was crucial. Generally, sufficiently high ionic strength suppresses interactions between hyaluronan and arginine oligomers, which demonstrated interactions in water. From the point of view of the molecular weight of hyaluronan, the transition between the rod conformation and the random coil conformation appeared to be important.

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Section: Discussionmentioning

confidence: 99%

Hyaluronan-Arginine Interactions—An Ultrasound and ITC Study

Jugl

Pekař

2020

Polymers

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“…The gel is a solid jelly-like material that can have properties ranging from soft and weak to hard, with a lot of potential applications, from cosmetics (e.g., gels containing hyaluronic acid for skin [28][29][30], shower gels, and hair gels) to solar cells and even flame retardants [31].…”

Section: Introductionmentioning

confidence: 99%

New Hybrid Materials Synthesized with Different Dyes by Sol-Gel Method

Gheonea

Crasmareanu

Pleşu

et al. 2017

Advances in Materials Science and Engineering

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One well-known method for hybrids synthesis with incorporated organic dyes is sol-gel method, which is based on the concept of molecular manipulation to design ceramics, glasses, and composites. The low-temperature process allows for the incorporation of guest organic molecules within the inorganic matrix, as well as for the synthesis of hybrid networks in which the organic and inorganic phases are interpenetrating. The aim of the work presented in this paper was the preparation of the gels with three different dyes, at different molar ratios by using the hydrolytic sol-gel process. The interaction of the dye and the oxide was examined by UV-vis spectroscopy and FT-IR. The thermal stability of the hybrid organic-inorganic xerogel formed here was studied by thermal analysis. The micrographs obtained by scanning electron microscopy (SEM) revealed the high density of the films. Such characteristics indicate the possible application of these films in solar cells.

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“…The main disadvantage of the former approach is finding suitable calibration standards that are structurally similar to the analyte. However, where suitable molar mass determination techniques are available, the conformation, as well as the temperature, pH, light, and storage time induced degradation of HA must also be investigated …”

Section: Introductionmentioning

confidence: 99%

“…However, where suitable molar mass determination techniques are available, the conformation, as well as the temperature, pH, light, and storage time induced degradation of HA must also be investigated. [25][26][27][28][29] For amphiphilic HA, DS alters the polymer's polarity, solubility, and aggregation behavior. Therefore, water or water-rich mobile phases, which are often used in the analysis of pure HA or HA with a low DS, become less suitable.…”

Section: Introductionmentioning

confidence: 99%

Molar Mass Analysis of Hydrophobically Modified Hyaluronic Acid by SEC‐MALLS: Facing the Challenges of Amphiphilic Biomacromolecules

Botha

Kuntz

Moire

et al. 2018

Macro Chemistry & Physics

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A size exclusion chromatography–multiangle laser light scattering (SEC‐MALLS) method is developed for determining the molar masses of amphiphilic hyaluronan (HA), consisting of a hydrophilic polymer backbone modified to varying extents with hydrophobic, polymerizable acrylate groups. The polymers are prepared via the base‐catalyzed esterification of HA with acrylic anhydride. Various parameters are investigated including mobile phase composition, temperature, pH, salt type, and concentration in order to find the most suitable conditions for completely dissolving the modified polymers irrespective of the degree of substitution (DS), and for suppressing secondary polymer interactions. The hydrolytic and the thermal stability of the different materials at various experimental conditions are also extensively investigated. HAs with DS varying from 0 to 3.4 are subsequently analyzed at the optimized experimental conditions for the mobile phase being DMSO/H2O 60/40(v/v) with 0.05 M LiBr and an operating temperature of 40 °C. The refractive index increments (dn/dc) of the HAs are determined and the results show a dependence of dn/dc on DS.

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Long-term degradation study of hyaluronic acid in aqueous solutions without protection against microorganisms

Cited by 23 publications

References 18 publications

Hyaluronan-Arginine Interactions—An Ultrasound and ITC Study

Hyaluronan-Arginine Interactions—An Ultrasound and ITC Study

New Hybrid Materials Synthesized with Different Dyes by Sol-Gel Method

Molar Mass Analysis of Hydrophobically Modified Hyaluronic Acid by SEC‐MALLS: Facing the Challenges of Amphiphilic Biomacromolecules

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