“…Previous studies reported various approaches to maintaining in vivo characteristics in cultured DP cells, including co-culture with keratinocytes Inamatsu et al, 1998;Kobayashi et al, 2011), cultivation on extracellular matrices, and sphere formation (Osada et al, 2007;Young et al, 2009;Higgins et al, 2010;Kobayashi et al, 2011). The hair inductive capacity of rodent and canine DP cells was maintained in cultures treated with keratinocyte-derived factors (Inamatsu et al, 1998;Kobayashi et al, 2011) and ligands in signaling pathways involved in hair follicle morphogenesis, including Wnt3a and Bmp6 (Kishimoto et al, 2000;Shimizu and Morgan, 2004;Rendl et al, 2008).…”