Long-term chemically protected sodium butyrate supplementation in broilers as an antibiotic alternative to dynamically modulate gut microbiota

Wan, Fan; Deng, Fuli; Chen, Lan-yue; Zhong, Ruqing; Wang, M.Y.; Yi, Bao; Liu, L.; Zhao, Huaibao; Zhang, H.F.

doi:10.1016/j.psj.2022.102221

Cited by 14 publications

(16 citation statements)

References 61 publications

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“…Over the past decades, using antibiotics in subtherapeutic doses as feed additives for animal and poultry diets has achieved multiple benefits of promoting growth, minimizing mortalities, and reducing production costs although, numerous safety worries emerged due to that practice, as the persistent inclusion of antibiotics in the food animal industry caused injury to animal’s intestines, suppression of gut health, contamination of animal products with antibiotic residues, and polluting of the environment with antibiotic-resistant bacteria [ 1 , 2 ]. All these drawbacks challenged animal producers to find efficient growth-promoting substitutes for antibiotics.…”

Section: Introductionmentioning

confidence: 99%

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Impact of buffered sodium butyrate as a partial or total dietary alternative to lincomycin on performance, IGF-1 and TLR4 genes expression, serum indices, intestinal histomorphometry, Clostridia, and litter hygiene of broiler chickens

Bawish,

Zahran,

Ismael

et al. 2023

Acta Vet Scand

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Background Sodium butyrate (SB) is a short-chain fatty acid and a safe antibiotic alternative. During 35 days, this study compared the impact of coated SB (Butirex C4) and lincomycin (Lincomix) on broiler growth, gut health, and litter hygiene in 1200 one-day-old Ross-308 broiler chicks that were randomly assigned into 5-dietary groups with 5-replications each. Groups divided as follows: T1: Basal diet (control), T2: Basal diet with buffered SB (1 kg/ton starter feed, 0.5 kg/ton grower-finisher feeds), T3: Basal diet with 100 g/ton lincomycin, T4: Basal diet with buffered SB (0.5 kg/ton starter feed, 0.25 kg/ton grower-finisher feeds) + 50 g/ton lincomycin, and T5: Basal diet with buffered SB (1 kg/ton starter feed, 0.5 kg/ton grower-finisher feeds) + 50 g/ton lincomycin. Birds were housed in a semi-closed deep litter house, where feed and water were available ad libitum. Results were statistically analyzed using ANOVA and Tukey’s post hoc tests. Results Combined dietary supplementation with SB and lincomycin (T4 and T5) significantly enhanced body weights, weight gains, feed conversion ratio, and profitability index. Also, carcasses in T4 and T5 exhibited the highest dressing, breast, thigh, and liver yields. T5 revealed the best blood biochemical indices, while T3 showed significantly elevated liver and kidney function indices. T4 and T5 exhibited the highest expression levels of IGF-1 and TLR4 genes, the greatest villi length of the intestinal mucosa, and the lowest levels of litter moisture and nitrogen. Clostridia perfringens type A alpha-toxin gene was confirmed in birds’ caeca, with the lowest clostridial counts defined in T4. Conclusions Replacing half the dose of lincomycin (50 g/ton) with 0.5 or 1 kg/ton coated SB as a dietary supplement mixture showed the most efficient privileges concerning birds’ performance and health.

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Section: Introductionmentioning

confidence: 99%

“…Previous studies indicated the application of coated SB at a rate of 600 to 1000 mg/kg of feed could enhance broiler chicken weights and feed conversion rates [ 2 , 10 ]. Further investigations reported that no significant body weight differences were obtained [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Impact of buffered sodium butyrate as a partial or total dietary alternative to lincomycin on performance, IGF-1 and TLR4 genes expression, serum indices, intestinal histomorphometry, Clostridia, and litter hygiene of broiler chickens

Bawish,

Zahran,

Ismael

et al. 2023

Acta Vet Scand

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“…Cytidine supplementation has been reported to improve dyslipidemia in obese mice via modulation of the microbiome with restoration of short chain fatty acid (SCFA) producing bacteria, including Lachnospiraceae 40,43 . Butyrate-producing bacteria regulate various gut-associated functions, including energy production, regulating gut barrier integrity by limiting pro-inflammatory cytokine production, and even inhibiting oncogenic pathways 44,45 . These bacteria also help maintain an anaerobic environment and prevent colonization of other pathogenic bacteria.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic-associated neutropenia is marked by depletion of intestinalLachnospiraceaein pediatric patients

Fernandez-Sanchez,

Rodgers,

Maknojia

et al. 2024

Preprint

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Hematologic side effects are associated with prolonged antibiotic exposure in up to 34% of patients. Neutropenia, reported in 10-15% of patients, increases the risk of sepsis and death. Murine studies have established a link between the intestinal microbiota and normal hematopoiesis. We sought to identify predisposing factors, presence of microbiota-derived metabolites, and changes in intestinal microbiota composition in otherwise healthy pediatric patients who developed neutropenia after prolonged courses of antibiotics. In this multi-center study, patients with infections requiring anticipated antibiotic treatment of two or more weeks were enrolled. Stool samples were obtained at the start and completion of antibiotics and at the time of neutropenia. We identified 10 patients who developed neutropenia on antibiotics and 29 controls matched for age, sex, race, and ethnicity. Clinical data demonstrated no association between neutropenia and type of infection or type of antibiotic used; however intensive care unit admission and length of therapy were associated with neutropenia. Reduced intestinal microbiome richness and decreased abundance of Lachnospiraceae family members correlated with neutropenia. Untargeted stool metabolomic profiling revealed several metabolites that were depleted exclusively in patients with neutropenia, including members of the urea cycle pathway, pyrimidine metabolism and fatty acid metabolism that are known to be produced by Lachnospiraceae. Our study confirms a relationship between intestinal microbiota disruption and abnormal hematopoiesis and identifies taxa and metabolites likely to contribute to microbiota-sustained hematopoiesis. As the microbiome is a key determinant of stem cell transplant and immunotherapy outcomes, these findings are likely to be of broad significance.

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“…Dietary 50 mg/kg and 150 mg/kg of aureomycin had no influence on the broilers’ growth performance (ADG and FCR) during the whole experiment, while ADFI from day 1 to 21 showed lower results in the latter [ 34 , 35 ]. Nevertheless, administrating broilers with 100 mg/kg of aureomycin enhanced BW and FCR [ 36 ]. These results suggested that the growth-prompting effects of antibiotics were not directly related to dose, which may be relevant considering that broad-spectrum antibiotic resistance causes increased tolerance in animals.…”

Section: Discussionmentioning

confidence: 99%

Effects of Clostridium butyricum as an Antibiotic Alternative on Growth Performance, Intestinal Morphology, Serum Biochemical Response, and Immunity of Broilers

Yang

Zhang

et al. 2023

Antibiotics

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The current study aimed to investigate the effects of Clostridium butyiricum on growth performance, intestinal morphology, serum biochemical response, and immunity in broiler chickens. A total of 330 commercial one-day-old, mixed-sex Ross 308 broilers were randomly divided into five treatment groups with six replicates per group. The broilers were fed the basal diet (CON), the basal diet with 150 mg/kg of aureomycin (AM), the basal diet with C. butyricum at 2 × 108 CFU/kg (CBL), the basal diet with C. butyricum at 4 × 108 CFU/kg (CBM), and the basal diet with C. butyricum at 8 × 108 CFU/kg (CBH). Results showed that the final body weight (BW) (p < 0.01; p < 0.05), ADG from day 22 to 39 (p < 0.05), and ADG from day 1 to 39 (p < 0.01; p < 0.05) were improved in a linear and quadratic response with the inclusion of C. butyricum. There were no differences in feed conversion rate (FCR) among all groups (p > 0.05). Supplementation with C. butyricum quadratically reduced the crypt depth at day 21 (p < 0.01), linearly improved the villus height in the jejunum at day 39 (p < 0.001), and linearly and quadratically increased the villus height to crypt depth (V/C) ratio in the jejunum at day 21 (p < 0.01) and day 39 (p < 0.01; p < 0.001). Dietary C. butyricum affected the thymus index at day 21 and day 39 (linear, p < 0.01), and the bursa of Fabricius index at day 39 (quadratic, p < 0.05). Compared to the AM group, the serum urea contents were decreased (p < 0.05) but the IgG contents were increased in the CBL and CBH groups at day 21 (p < 0.01); in addition, serum albumin (ALB) concentrations in all the C. butyricum-supplemented groups (p < 0.01) and IgG concentrations in the CBM group were augmented at day 39 (p < 0.05). In conclusion, dietary C. butyricum could enhance growth performance by improving jejunal morphology and stimulating immunity organ development in broilers, and could be an alternative to antibiotics in poultry feeds.

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Long-term chemically protected sodium butyrate supplementation in broilers as an antibiotic alternative to dynamically modulate gut microbiota

Cited by 14 publications

References 61 publications

Impact of buffered sodium butyrate as a partial or total dietary alternative to lincomycin on performance, IGF-1 and TLR4 genes expression, serum indices, intestinal histomorphometry, Clostridia, and litter hygiene of broiler chickens

Impact of buffered sodium butyrate as a partial or total dietary alternative to lincomycin on performance, IGF-1 and TLR4 genes expression, serum indices, intestinal histomorphometry, Clostridia, and litter hygiene of broiler chickens

Antibiotic-associated neutropenia is marked by depletion of intestinalLachnospiraceaein pediatric patients

Effects of Clostridium butyricum as an Antibiotic Alternative on Growth Performance, Intestinal Morphology, Serum Biochemical Response, and Immunity of Broilers

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