Long-term changes to in vitro preserved bioengineered human trachea and their implications for decellularized tissues

Baiguera, Silvia; Gaudio, Costantino Del; Jaus, Massimo Osvaldo; Polizzi, Leonardo; Gonfiotti, Alessandro; Comin, Camilla E.; Bianco, Alessandra; Ribatti, Doménico; Taylor, Doris A.; Macchiarini, Paolo

doi:10.1016/j.biomaterials.2012.01.064

Cited by 79 publications

(75 citation statements)

References 40 publications

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“…To date, several ECM products have been used or tested in multiple clinical applications [Badylak et al, 2009]. In the lab, whole-organ ECM scaffolds with intact 3D anatomical structures and vasculature conduits have been generated from the heart, lungs, liver, kidneys, pancreas and other organs [Baiguera et al, 2012;Bonvillain et al, 2012;Jeng et al, 2013;Mirmalek-Sani et al, 2013;O'Neill et al, 2013]. Studies have shown that ECM scaffold acquired by decellularization can be repopulated with cells, cultured as a whole-organ construct and partially restore organ function after orthotopic transplantation Totonelli et al, 2012;Qu et al, 2013;Ren et al, 2013].…”

Section: Discussionmentioning

confidence: 99%

Generation of Bioartificial Salivary Gland Using Whole-Organ Decellularized Bioscaffold

Gao

et al. 2014

Cells Tissues Organs

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Salivary gland hypofunction resulting in xerostomia occurs as a result of various pathological conditions such as radiotherapy for head and neck cancers, Sjögren's syndrome or salivary gland tumor resection. It can induce a large number of problems, including dental decay, periodontitis, dysgeusia, difficulty with mastication and swallowing and a reduced quality of life. Current therapies for xerostomia mostly focus on saliva substitutes, oral lubricants and medications which stimulate salivation from residual glands. However, these treatments are not sufficient to restore gland secretory function. Tissue engineering-based organ regeneration has emerged as a potential therapeutic alternative for end- organ failure. Here, we decellularized rat submandibular glands (SMG) by detergent immersion. Histological, immunofluorescent, Western blot, DNA and collagen quantitative analyses demonstrated that our protocol effectively removed cellular components and that extracellular matrix proteins and native structures were well preserved. We then reseeded the decellularized SMG as scaffolds with rat primary SMG cells in a rotary cell culture system. Histological staining and electron microscopy analyses illustrated that the decellularized SMG could support cellular adhesion. Furthermore, with immunofluorescent staining, we proved that bioartificially generated SMG showed some differentiation markers in vitro. Taken together, our findings might provide a potential scaffold for tissue-engineered regeneration of the salivary glands.

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Section: Discussionmentioning

confidence: 99%

Generation of Bioartificial Salivary Gland Using Whole-Organ Decellularized Bioscaffold

Gao

et al. 2014

Cells Tissues Organs

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“…This is because it is of paramount importance that both decellularised scaffolds and recellularised, bioengineered replacements can be stored for prolonged periods of time prior to clinical use. For example, Baiguera et al [18] studied the effects of long-term (1 year) storage on both the microstructure and mechanical properties of human decellularised tracheas. To investigate the impact of storing the samples at 4…”

Section: Resultsmentioning

confidence: 99%

Comparative quasi-static mechanical characterization of fresh and stored porcine trachea specimens

Butler

Williams

Tucker

et al. 2018

Eur. Phys. J. Spec. Top.

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Abstract. Tissues of the upper airways of critically ill patients are particularly vulnerable to mechanical damage associated with the use of ventilators. Ventilation is known to disrupt the structural integrity of respiratory tissues and their function. This damage contributes to the vulnerability of these tissues to infection. We are currently developing tissue models of damage and infection to the upper airways. As part of our studies, we have compared how tissue storage conditions affect mechanical properties of excised respiratory tissues using a quasi-static platform. Data presented here show considerable differences in mechanical responses of stored specimens compared to freshly excised specimens. These data indicate that implementation of storage and maintenance procedures that minimize rapid degradation of tissue structure are essential for retaining the material properties in our tissue trauma models.

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“…The ideal method of decellularisation remains to be determined, with differences seen in structural integrity and ECM composition between the three most popular protocols (139). Tissue heterogeneity due to long-term storage prior to surgical implantation is another caveat that must be taken into account (140). Finally, there is the risk of disease transmission between donor and recipient.…”

Section: Decellularised Tissuementioning

confidence: 99%

Respiratory Tissue Engineering: Current Status and Opportunities for the Future

O’Leary

Gilbert

O’Dea

et al. 2015

Tissue Engineering Part B: Reviews

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Long-term changes to in vitro preserved bioengineered human trachea and their implications for decellularized tissues

Cited by 79 publications

References 40 publications

Generation of Bioartificial Salivary Gland Using Whole-Organ Decellularized Bioscaffold

Generation of Bioartificial Salivary Gland Using Whole-Organ Decellularized Bioscaffold

Comparative quasi-static mechanical characterization of fresh and stored porcine trachea specimens

Respiratory Tissue Engineering: Current Status and Opportunities for the Future

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