Long-Term Cell Suspension Culture and Regeneration of the Single-Leafed StrawberryFragaria vesca monophylla

Abstract

Cell suspensions have been used for obtaining somaclonal variability and for the production of secondary metabolites, such as aroma compounds, natural dyes and drugs. Undifferentiated growing cells completely lose their regeneration capacity due to the formation of cells with abnormal DNA content during this phase. Callus culture of Fragaria vesca monophyllawas obtained on agarised media supplemented with different growth regulators. Afterwards, a stable cell suspension was obtained on an SH (1972) medium supplemented with BSAA 0·25 mg litre−1 and BA 0·1 mg litre−1. The cells were poured onto an agarised regeneration medium, where it was possible to obtain regenerants. This is the first report of a stable cell suspension of a diploidFragariaand regeneration after 2 years of unorganised cell culture.