Long-Range Cooperative Disassembly and Aging During Adenovirus Uncoating

Martín-González, Natalia; Ibáñez‐Freire, Pablo; Ortega-Esteban, Álvaro; Laguna-Castro, Mara; Martín, Carmen San; Valbuena, Alejandro; Delgado‐Buscalioni, Rafael; Pablo, Pedro

doi:10.1103/physrevx.11.021025

Cited by 11 publications

(20 citation statements)

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“…We believe that another possibility is that the spikes yield under the AFM tip force (~80 pN), reaching the viral capsid with the apex of the AFM stylus. This effect has also been recently found in human adenovirus [ 64 ], where the fibers remained invisible to AFM due to their great flexibility, despite their large size. However, fibers can be imaged when they are short and thick and robustly bonded to the capsid, as is the case of human rotavirus, where spikes can be visualized over a few frames before being wiped out during the imaging process [ 28 ].…”

Section: Discussionsupporting

confidence: 56%

“…For statistical analysis, we monitored eight particles subjected to similar processes and plotted their individual ( Figure 4 B, thin grey) and average ( Figure 4 B, thick dark) height variation over time. It is known that the AFM imaging process itself induces the disruption of some viruses, such as lambda phage and human adenovirus [ 31 , 64 ]. To rule out that effect as being responsible for the decrease in height, consecutive images of virions in buffer without detergent were taken as control ( Figure 4 B, blue, Video S5 ), showing that their heights were not affected by AFM scanning.…”

Section: Resultsmentioning

confidence: 99%

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Monitoring SARS-CoV-2 Surrogate TGEV Individual Virions Structure Survival under Harsh Physicochemical Environments

Cantero

Carlero

Chichón

et al. 2022

Cells

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Effective airborne transmission of coronaviruses via liquid microdroplets requires a virion structure that must withstand harsh environmental conditions. Due to the demanding biosafety requirements for the study of human respiratory viruses, it is important to develop surrogate models to facilitate their investigation. Here we explore the mechanical properties and nanostructure of transmissible gastroenteritis virus (TGEV) virions in liquid milieu and their response to different chemical agents commonly used as biocides. Our data provide two-fold results on virus stability: First, while particles with larger size and lower packing fraction kept their morphology intact after successive mechanical aggressions, smaller viruses with higher packing fraction showed conspicuous evidence of structural damage and content release. Second, monitoring the structure of single TGEV particles in the presence of detergent and alcohol in real time revealed the stages of gradual degradation of the virus structure in situ. These data suggest that detergent is three orders of magnitude more efficient than alcohol in destabilizing TGEV virus particles, paving the way for optimizing hygienic protocols for viruses with similar structure, such as SARS-CoV-2.

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Section: Discussionsupporting

confidence: 56%

Section: Resultsmentioning

confidence: 99%

Monitoring SARS-CoV-2 Surrogate TGEV Individual Virions Structure Survival under Harsh Physicochemical Environments

Cantero

Carlero

Chichón

et al. 2022

Cells

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“…As the force increased, the virus container yielded, and the FZ showed a non-linear stepwise regime (Figure 3B,C, black) that corresponded to the unzipping events between the capsomers. [46][47][48] This caused the fracture between some VLP subunits [49] that were able to recover their spherical shape following the removal of the tip. [50][51][52][53]…”

Section: Vlp Indentationmentioning

confidence: 99%

Electromechanical Photophysics of GFP Packed Inside Viral Protein Cages Probed by Force‐Fluorescence Hybrid Single‐Molecule Microscopy

Strobl

Selivanovitch

Ibáñez‐Freire

et al. 2022

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Packing biomolecules inside virus capsids has opened new avenues for the study of molecular function in confined environments. These systems not only mimic the highly crowded conditions in nature, but also allow their manipulation at the nanoscale for technological applications. Here, green fluorescent proteins are packed in virus‐like particles derived from P22 bacteriophage procapsids. The authors explore individual virus cages to monitor their emission signal with total internal reflection fluorescence microscopy while simultaneously changing the microenvironment with the stylus of atomic force microscopy. The mechanical and electronic quenching can be decoupled by ≈10% each using insulator and conductive tips, respectively. While with conductive tips the fluorescence quenches and recovers regardless of the structural integrity of the capsid, with the insulator tips quenching only occurs if the green fluorescent proteins remain organized inside the capsid. The electronic quenching is associated with the coupling of the protein fluorescence emission with the tip surface plasmon resonance. In turn, the mechanical quenching is a consequence of the unfolding of the aggregated proteins during the mechanical disruption of the capsid.

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“…[1][2][3][4][5][6][7][8] For a few decades, the physical virology community has employed diverse experimental techniques, like force microscopy, to learn more about the mechanical and electrostatic properties of biomacromolecular systems. [9][10][11][12][13][14][15][16][17][18][19] However, the amount of viruses that are electrostatically characterized at the nanoscale (i.e. few nanometers) are scarce, where we can highlight the pioneering work from de Pablo and co-workers.…”

Section: Introductionmentioning

confidence: 99%

“…particular, the interactions at the atomistic and molecular (subnanometric) resolutions are of predominant interests in proteinaceous capsids, membranes and whole viruses [1][2][3][4][5][6][7][8] . For a few decades, the physical virology community has employed diverse experimental techniques, like force microscopy, to learn more about the mechanical and electrostatic properties of biomacromolecular systems [9][10][11][12][13][14][15][16][17][18][19] . However, the amount of viruses that are electrostatically characterized at the nanoscale (i.e.…”

mentioning

confidence: 99%

Quantitative electrostatic force tomography for virus capsids in interaction with an approaching nanoscale probe

2022

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Long-Range Cooperative Disassembly and Aging During Adenovirus Uncoating

Cited by 11 publications

References 50 publications

Monitoring SARS-CoV-2 Surrogate TGEV Individual Virions Structure Survival under Harsh Physicochemical Environments

Monitoring SARS-CoV-2 Surrogate TGEV Individual Virions Structure Survival under Harsh Physicochemical Environments

Electromechanical Photophysics of GFP Packed Inside Viral Protein Cages Probed by Force‐Fluorescence Hybrid Single‐Molecule Microscopy

Quantitative electrostatic force tomography for virus capsids in interaction with an approaching nanoscale probe

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