Long noncoding <scp>RNA</scp> actin filament‐associated protein 1 antisense <scp>RNA</scp> 1 promotes malignant phenotype through binding with lysine‐specific demethylase 1 and repressing <scp>HMG</scp> box‐containing protein 1 in non‐small‐cell lung cancer

Yu, Shanxun; Yang, Dongxue; Ye, Yunyao; Liu, Pei; Chen, Zhenyao; Lei, Tianyao; Pu, Jiaze; Liu, Longfa; Wang, Zhaoxia

doi:10.1111/cas.14039

Cited by 27 publications

(16 citation statements)

References 37 publications

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“…The mechanism of action of lncRNAs is mainly associated with their subcellular localisation. Nuclear lncRNAs mostly show effects at the transcriptional levels, while cytoplasmic lncRNAs mostly function as endogenous sponges for miRNAs or show effects at post-transcriptional levels (22,23). Furthermore, FISH assays showed that LINC01554 is mainly located in the nuclei of HCC cells, indicating its function in transcriptional regulation.…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics analysis of LINC01554 and its co‑expressed genes in hepatocellular carcinoma

Huang

et al. 2020

Oncol Rep

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Hepatocellular carcinoma (HCC) is a leading cause of cancer-related morbidity and mortality globally. Despite the remarkable improvements in comprehensive HCC treatment, the underlying mechanistic details of HCC remain elusive. We screened HCC patients for differentially expressed genes (DEGs) using the Gene Expression Omnibus (GSE113850) and The Cancer Genome Atlas (TCGA) datasets. LINC01554 expression in 40 paired samples was determined by quantitative reverse transcription polymerase chain reaction (RT-qPCR), and its clinical significance was assessed. LINC01554 was found to have a gain-of-function role in HCC in vitro. Additionally, the bioinformatics analysis of the genes co-expressed with LINC01554 was performed using the Co-LncRNA website, and potential molecular mechanisms were investigated using the Gene Ontology and Kyoto Encyclopaedia of Genes and Genomes resources and validated by in vitro experiments. A total of 229 DEGs were identified from the GSE113850 dataset. Among the identified DEGs, three long non-coding RNAs (lncRNAs) (DIO3OS, LINC01554, and LINC01093) with |logFC| ≥2 and P<0.05 were screened. A total of 148 lncRNAs with |logFC| ≥1 and P<0.05 were identified from TCGA dataset. Low LINC01554 expression levels were significantly correlated with overall survival, pathological stage, hepatitis B infection, tumour size, portal vein tumour thrombus, and TNM stage. Using gain-of-function assays, we further showed that LINC01554 inhibited the proliferation, migration, and invasion of the HCCLM9 and SK-Hep1 cells and promoted G0/G1 arrest, but it did not significantly affect apoptosis. Western blotting revealed that LINC01554 overexpression resulted in increased ZO-1 and E-cadherin expression levels, but decreased N-cadherin and vimentin expression levels. Moreover, LINC01554 overexpression inhibited Akt, p-Akt, β-catenin, and p-Gsk3β expression. Our results showed that LINC01554 repressed HCC cell invasiveness and epithelial-to-mesenchymal transition partly by inhibiting Wnt and PI3K-Akt signalling in vitro. Taken together, our findings provide new insights into the molecular mechanisms underlying HCC tumourigenesis and implicate LINC01554 as a potential target for HCC therapy.

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Section: Discussionmentioning

confidence: 99%

Bioinformatics analysis of LINC01554 and its co‑expressed genes in hepatocellular carcinoma

Huang

et al. 2020

Oncol Rep

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“…AFAP1-AS1 is upregulated in NSCLC tissues and cell lines [91,99,100,101,102,103,104,105,106,107]. Inhibition of AFAP-AS1 suppresses cell growth and migration and promotes apoptosis of NSCLC cells in vitro [103,104]. AFAP1-AS1 knockdown leads to a significant increase in keratin 1 (KRT1) expression in vitro, suggesting that the oncogenic activity of AFAP1-AS1 is mediated by suppressing KRT1 expression [104].…”

Section: Lncrnas In Lung Cancer (Lc)mentioning

confidence: 99%

Long Non-Coding RNA in the Pathogenesis of Cancers

Chi

Wang

et al. 2019

Cells

608

452

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The incidence and mortality rate of cancer has been quickly increasing in the past decades. At present, cancer has become the leading cause of death worldwide. Most of the cancers cannot be effectively diagnosed at the early stage. Although there are multiple therapeutic treatments, including surgery, radiotherapy, chemotherapy, and targeted drugs, their effectiveness is still limited. The overall survival rate of malignant cancers is still low. It is necessary to further study the mechanisms for malignant cancers, and explore new biomarkers and targets that are more sensitive and effective for early diagnosis, treatment, and prognosis of cancers than traditional biomarkers and methods. Long non-coding RNAs (lncRNAs) are a class of RNA transcripts with a length greater than 200 nucleotides. Generally, lncRNAs are not capable of encoding proteins or peptides. LncRNAs exert diverse biological functions by regulating gene expressions and functions at transcriptional, translational, and post-translational levels. In the past decade, it has been demonstrated that the dysregulated lncRNA profile is widely involved in the pathogenesis of many diseases, including cancer, metabolic disorders, and cardiovascular diseases. In particular, lncRNAs have been revealed to play an important role in tumor growth and metastasis. Many lncRNAs have been shown to be potential biomarkers and targets for the diagnosis and treatment of cancers. This review aims to briefly discuss the latest findings regarding the roles and mechanisms of some important lncRNAs in the pathogenesis of certain malignant cancers, including lung, breast, liver, and colorectal cancers, as well as hematological malignancies and neuroblastoma.

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“…Rescue test determined that the epigenetic silencing of HBP1 could be induced by AFAP1-AS1 and is partly related to the oncogenic impact of AFAP1-AS1. 21 Yin et al reported that AFAP1-AS1 could epigenetically silence p21 transcription, which is a mediator of p53-dependent G1 growth in A549 cells. 22,23 Also, it is shown that the knockdown of the AFAP1-AS1 increased the AFAP1 expression and affected some small GTPase family molecules expressions in the actin cytokeratin signaling pathway and, therefore, it seems that AFAP1-AS1 induces tumor cell migration through controlling of the actin filament integrity.…”

Section: Afap1-as1mentioning

confidence: 99%

“…AFAP1‐AS1 suppresses HMG box‐containing protein 1 (HBP1), which is a tumor suppressor and its expression leads to reduced cell growth and progression in PC‐9 and H1975 cell lines. Rescue test determined that the epigenetic silencing of HBP1 could be induced by AFAP1‐AS1 and is partly related to the oncogenic impact of AFAP1‐AS1 21 . Yin et al reported that AFAP1‐AS1 could epigenetically silence p21 transcription, which is a mediator of p53‐dependent G1 growth in A549 cells 22,23 .…”

Section: Introductionmentioning

confidence: 99%

Long non‐coding RNAs as potential biomarkers in the prognosis and diagnosis of lung cancer: A review and target analysis

et al. 2020

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Long non-coding RNAs (lncRNA) have been emerged as a novel class of molecular regulators in cancer. They are dysregulated in many types of cancer; however, there is not enough knowledge available on their expression and functional profiles. Lung cancer is the leading cause of the cancer deaths worldwide. Generally, lncRNAs may be associated with lung tumor pathogenesis and they may act as biomarkers for the cancer prognosis and diagnosis. Compared to other invasive prognostic and diagnostic methods, detection of lncRNAs might be a user-friendly and noninvasive method. In this review article, we selected 27 tumor-associated lncRNAs by literature reviewing to further discussing in detail for using as diagnostic and prognostic biomarkers in lung cancer. Also, in an in silico target analysis, the "Experimentally supported functional regulation" approach of the LncTarD web tool was used to identifying the target genes and regulatory mechanisms of the selected lncRNAs. The reports on diagnostic and prognostic potential of all selected lncRNAs were discussed. However, the target genes and regulatory mechanisms of the 22 lncRNAs were identified by in silico analysis and we found the pathways that are controlled by each target group of lncRNAs. They use epigenetic mechanisms, ceRNA mechanisms, protein interaction and sponge mechanism. Also, 10, 23, 5, and 28 target genes for each of these mechanisms were identified, respectively. Finally, each group of target genes controls 50, 12, 7, and 2 molecular pathways, respectively. In conclusion, LncRNAs could be used as

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Long noncoding RNA actin filament‐associated protein 1 antisense RNA 1 promotes malignant phenotype through binding with lysine‐specific demethylase 1 and repressing HMG box‐containing protein 1 in non‐small‐cell lung cancer

Cited by 27 publications

References 37 publications

Bioinformatics analysis of LINC01554 and its co‑expressed genes in hepatocellular carcinoma

Bioinformatics analysis of LINC01554 and its co‑expressed genes in hepatocellular carcinoma

Long Non-Coding RNA in the Pathogenesis of Cancers

Long non‐coding RNAs as potential biomarkers in the prognosis and diagnosis of lung cancer: A review and target analysis

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