Long Noncoding RNAs Expression Profile of the Developing Mouse Heart

Zhu, Jin; Shen, Yanying; Liu, Hai Lang; Liu, Ming; Shen, Ya Qing; Kong, Xiang; Song, Gui Xian; Qian, Ling

doi:10.1002/jcb.24733

Cited by 35 publications

(19 citation statements)

References 30 publications

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“…These highly dynamic changes of lncRNA expressions during the early postnatal stage suggested their crucial roles in cardiac adaptation response after the birth, such as to the oxygenation state, neuroendocrine stimulation41, changes in hemodynamics42, and lactation343. GO and pathway analysis of neighboring genes showed that the major biological processes and pathways were predominantly associated with cardiac development and growth, including cell cycle regulation, DNA-templated transcription, as well as AMPK signaling pathway as previously reported27 and also in consistent with recent studies that lncRNAs in developing embryos were mainly involved in cell cycle regulation, cell differentiation, and cell fate commitment38. Furthermore, our STC analysis showed that lncRNAs in Profile #9 and #4 were persistently downregulated from P1 to P28, suggesting that these transcripts might mainly act on the embryonic heart development.…”

Section: Discussionsupporting

confidence: 87%

“…Non-coding genes constitute the majority of human genome while coding genes only account for 1.2%35. Previously, several elegant studies have identified cardiac-enriched lncRNAs in cardiovascular lineage commitment12, embryonic heart development1836373839, and cardiovascular diseases such as cardiac hypertrophy and heart failure1640. Our current study is focused on the expression profiles of lncRNAs in the hearts during the early postnatal growth stage, which is associated with the critical transition from hyperplastic to hypertrophic growth with dynamic cellular processes orchestrated by a complex signal network123.…”

Section: Discussionmentioning

confidence: 99%

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Profiling analysis of long non-coding RNAs in early postnatal mouse hearts

Sun

Han

Luo

et al. 2017

Sci Rep

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Mammalian cardiomyocytes undergo a critical hyperplastic-to-hypertrophic growth transition at early postnatal age, which is important in establishing normal physiological function of postnatal hearts. In the current study, we intended to explore the role of long non-coding (lnc) RNAs in this transitional stage. We analyzed lncRNA expression profiles in mouse hearts at postnatal day (P) 1, P7 and P28 via microarray. We identified 1,146 differentially expressed lncRNAs with more than 2.0-fold change when compared the expression profiles of P1 to P7, P1 to P28, and P7 to P28. The neighboring genes of these differentially expressed lncRNAs were mainly involved in DNA replication-associated biological processes. We were particularly interested in one novel cardiac-enriched lncRNA, ENSMUST00000117266, whose expression was dramatically down-regulated from P1 to P28 and was also sensitive to hypoxia, paraquat, and myocardial infarction. Knockdown ENSMUST00000117266 led to a significant increase of neonatal mouse cardiomyocytes in G0/G1 phase and reduction in G2/M phase, suggesting that ENSMUST00000117266 is involved in regulating cardiomyocyte proliferative activity and is likely associated with hyperplastic-to-hypertrophic growth transition. In conclusion, our data have identified a large group of lncRNAs presented in the early postnatal mouse heart. Some of these lncRNAs may have important functions in cardiac hyperplastic-to-hypertrophic growth transition.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Profiling analysis of long non-coding RNAs in early postnatal mouse hearts

Sun

Han

Luo

et al. 2017

Sci Rep

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show abstract

“…Building on previous studies that detected differentially expressed lncRNAs during mouse heart development, 6, 7, 9, 31, 38 cardiac cell differentiation, 39 and heart disease, 8, 40 we report here the contrasting “lncRNA-omes” between human fetal and adult hearts. Furthermore, through expression correlations between lncRNAs and protein coding mRNAs based on genomic proximity, we construct a co-expression network that enables identification of important coding genes and lncRNAs in human heart development.…”

Section: Discussionmentioning

confidence: 82%

Systematic Characterization of Long Noncoding RNAs Reveals the Contrasting Coordination of Cis - and Trans -Molecular Regulation in Human Fetal and Adult Hearts

Wilson

et al. 2016

Circ Cardiovasc Genet

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Background The molecular regulation of heart development is regulated by cis- and trans-factors acting on the genome and epigenome. As a class of important regulatory RNAs, the role of long non-coding RNAs (lncRNAs) in human heart development is still poorly understood. Furthermore, factors that interact with lncRNAs in this process are not well characterized. Methods and Results Utilizing RNA sequencing, we systematically define the contrasting lncRNA expression patterns between fetal and adult heart. We report that lncRNAs up-regulated in adult versus fetal heart have different sequence features and distributions. For example, the adult heart expresses more sense lncRNAs compared to fetal heart. We also report the co-expression of lncRNAs and neighboring coding genes that have important functions in heart development. Importantly, the regulation of lncRNA expression during fetal to adult heart development appears to be due in part to the coordination of specific developmental epigenetic modifications such as H3K4me1 and H3k4me3. The expression of promoter-associated lncRNAs in adult and fetal heart also appears to be related to these epigenetic states. Finally, transcription factor binding analysis suggests that lncRNAs are directly regulating cardiac gene expression during development. Conclusions We provide a systematic analysis of lncRNA control of heart development that gives clues to the roles that specific lncRNAs play in fetal and adult hearts.

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“…To this end, the transcriptomes of whole hearts from E11.5, E14.5, and E18.5, time points representing critical developmental transitions, were profiled to identify temporally expressed lncRNAs in the developing fetal mouse heart. 58 Several hundred lncRNAs displayed distinct expression changes between the embryonic time points, providing a preliminary list of potentially important lncRNAs in early cardiac development. Whether there are overlapping lncRNAs among these studies remains to be explored.…”

Section: Dynamic Lncrna Expression During Normal Heart Developmentmentioning

confidence: 99%

Lnc ing Epigenetic Control of Transcription to Cardiovascular Development and Disease

Rizki

Boyer

2015

Circulation Research

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Transcriptional and epigenetic regulation is critical for proper heart development, cardiac homeostasis, and pathogenesis. Long noncoding RNAs have emerged as key components of the transcriptional regulatory pathways that govern cardiac development as well as stress response, signaling, and remodeling in cardiac pathologies. Within the past few years, studies have identified many long noncoding RNAs in the context of cardiovascular biology and have begun to reveal the key functions of these transcripts. In this review, we discuss the growing roles of long noncoding RNAs in different aspects of cardiovascular development as well as pathological responses during injury or disease. In addition, we discuss diverse mechanisms by which long noncoding RNAs orchestrate cardiac transcriptional programs. Finally, we explore the exciting potential of this novel class of transcripts as biomarkers and novel therapeutic targets for cardiovascular diseases. ( Rizki and Boyer Long Noncoding RNAs in Cardiovascular Biology 193of fetal cardiac and stress response genes. 6,8,9 Despite these findings, transcriptional and epigenetic reprogramming of the failing heart is a complex process and the full spectrum of molecular determinants of these responses are waiting to be identified. Similar to developmental control mechanisms, noncoding RNAs are emerging players in post injury response cascades. In this review, we focus on the surge of recent studies that have begun to uncover the critical roles of lncRNAs in many different aspects of cardiovascular development, physiology, and disease.

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Long Noncoding RNAs Expression Profile of the Developing Mouse Heart

Cited by 35 publications

References 30 publications

Profiling analysis of long non-coding RNAs in early postnatal mouse hearts

Profiling analysis of long non-coding RNAs in early postnatal mouse hearts

Systematic Characterization of Long Noncoding RNAs Reveals the Contrasting Coordination of Cis - and Trans -Molecular Regulation in Human Fetal and Adult Hearts

Lnc ing Epigenetic Control of Transcription to Cardiovascular Development and Disease

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