Long Noncoding RNA UCA1 Is Related to Autophagy and Apoptosis in Endometrial Stromal Cells

Abstract

Research QuestionThe expression of the long noncoding RNA (lncRNA) urothelial carcinoma-associated 1 (UCA1) in embryonic tissues is higher than that in most cancer tissues, such as bladder cancer, indicating that RNA is a carcinoembryonic antigen. However, there are no published reports on the role of UCA1 in endometriosis (EMS). Therefore, to address this gap in knowledge, we assessed the potential role of lncRNA UCA1 in the pathogenesis and progression of EMS.DesignTo verify the expression of UCA1 in EMS, quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used. RNA interference (siRNA) was used to study the biological function of UCA1 in EMS in vitro.ResultsqRT-PCR analysis showed that the expression of lncRNA UCA1 in EMS was increased (P<0.01). Knockdown of UCA1 in vitro significantly inhibited the proliferation of endometrial stromal cells (ESCs) and induced autophagy and apoptosis.ConclusionUCA1 is highly expressed in EMS and promotes the proliferation of ESCs but suppresses autophagy and apoptosis. In EMS, UCA1 may be a prognostic marker and therapeutic target.

IGF2BP3 promotes glutamine metabolism of endometriosis by interacting with UCA1 to enhances the mRNA stability of GLS1

“…Through analyzing the RIP-seq dataset and GEO database,we identified UCA1 as an interaction partner of IGF2BP3, which was initially recognized as a promoter of bladder cancer (Li et al 2015 ). Liu et al have confirmed that UCA1 promoted the proliferation of eESCs, while inhibiting autophagy and apoptosis (Jiang et al 2020 ). Subcellular localization is a crucial factor in determining the biological function of lncRNAs.…”

Analysis of Long Non-Coding RNA (lncRNA) UCA1, MALAT1, TC0101441, and H19 Expression in Endometriosis

“…It is known that UCA1 may be involved in the occurrence and development of endometriosis by promoting cell proliferation and inhibiting apoptosis [ 27 ].…”

Review of the Potential Therapeutic Effects and Molecular Mechanisms of Resveratrol on Endometriosis

“…According to literature, the formation and maintenance of ectopic endometrial lesions at ectopic sites are the most basic characteristics of endometriosis and are strongly correlated with the pathology and pathogenesis, such as inflammation, oxidative stress, proliferation, apoptosis, angiogenesis, immunity, invasion, and adhesion. 42–47 It has also been found that some other factors, such as hormones, heredity, and environment, are implicated in the formation and/or advancement of this disease. Local estrogen augmentation and progesterone resistance are observed in endometriosis, thus promoting the survival, migration, proliferation, and adhesion of ectopic endometrial cells.…”

“…Endometriosis is a proliferative chronic disorder that is mainly characterized by the abnormal endometrial growth at ectopic sites outside the uterine cavity. 42 Previous studies have shown an incremental cell proliferation rate of ectopic endometrium in endometriotic patients, which is conductive to survival and implantation into the ectopic site. 50 Proliferating cell nuclear antigen (PCNA), a sensitive indicator of proliferation and DNA restoration, exists in the G1, S and G2 phases of the cell cycle, while Ki-67 exists in all active phases and is a more specific proliferative marker than PCNA.…”

“…proliferation, apoptosis, angiogenesis, immunity, invasion, and adhesion. [42][43][44][45][46][47] It has also been found that some other factors, such as hormones, heredity, and environment, are implicated in the formation and/or advancement of this disease. Local estrogen augmentation and progesterone resistance are observed in endometriosis, thus promoting the survival, migration, proliferation, and adhesion of ectopic endometrial cells.…”