Long noncoding RNA SNHG20 promotes gastric cancer progression by inhibiting p21 expression and regulating the GSK-3β/ β-catenin signaling pathway

Abstract

Recent studies have indicated that long non-coding RNAs (lncRNAs) play important regulatory roles in tumor development and progression. However, the contribution of small nucleolar RNA host gene 20 (SNHG20) to gastric cancer development remains largely unknown. The aim of the study is to investigate the functional significance of SNHG20 involved in gastric cancer (GC) progression. In the study, our results demonstrated that the expression levels of SNHG20 were remarkably up-regulated in GC cells. Functionally, SNHG20 promoted the GC MKN45 and BGC-823 cells proliferation and invasion. Furthermore, knockdown of SNHG20 significantly inhibited the epithelial-mesenchymal transition (EMT) in MKN45 and BGC-823 cells, whereas, the overexpression of SNHG20 had the promoting effects. Moreover, we found that overexpression of SNHG20 in MKN45 and BGC-823 cells significantly inhibited the expression of E-cadherin and p21 via binding to EZH2 and regulated the GSK-3β/β-catenin signaling pathway. Thus, the results showed that SNHG20 acted as an oncogene in GC and targeting SNHG20 may serve as a therapeutic target for GC.

LncRNA SNHG20 is associated with clinical progression and enhances cell migration and invasion in osteosarcoma

“…In colorectal cancer, Li et al indicated that levels of SNHG20 expression were up‐regulated in tumor tissues compared to corresponding normal tissues from 107 . Besides, high‐expression of SNHG20 was observed in human gastric cancer cell lines . Similar results about the high‐expression status of SNHG20 expression were found in female common cancers including cervical cancer , ovarian cancer and breast cancer .…”

“…Besides, Li et al revealed silencing of SNHG20 inhibited cell proliferation, invasion, and migration, and cell cycle progression in colorectal cancer . The oncogenic function of SNHG20 was also observed in gastric cancer , cervical cancer , ovarian cancer , and breast cancer .…”

“…The epithelial–mesenchymal transition (EMT) is a complex and multi‐step biological process that serves as a key role in modulating tumor cell metastasis . SNHG20 has been showed to be involved in EMT process . In order to explore the molecular mechanism of SNHG20 in regulating osteosarcoma cell migration and invasion, we conducted qRT‐PCR to screen the mRNA expression of EMT‐associated molecules including Snail, Slug, ZEB1, ZEB2, E‐Cadherin, and Vimentin.…”

SNHG20: A vital lncRNA in multiple human cancers

“…ZEB1, ZEB2, N-cadherin, vimentin,E-cadherin, and EZH2 D. Zhang et al (2016E-Cadherin, p21, EZH2, H3k27me3, p-GSK-3βLiu et al (2017) Non-small-cell lung cancer cancer process by facilitating Wnt/β-catenin signaling. Owing to its function, lncRNA SNHG20 could also be identified as one of the oncogenes among ovarian cancer biomarkers.…”

Long non‑coding RNA DLX6‑AS1 silencing inhibits malignant phenotypes of gastric cancer cells

“…This therefore reveals that furthering understanding into the underlying mechanisms of GC may be beneficial for the development of novel and effective therapies (27,2�). Previous studies have reported that certain lncRNAs, including Small nuclear RNA host gene 20 (SNHG20) (29), terminal differentiation-induced ncRNA (30), nuclear paraspeckle assembly transcript 1 (NEAT1) (31) and XIST (15) are aberrantly expressed in GC and regulate tumour growth and metastasis. For instance, NEAT1 is an unfavourable prognostic factor in GC and serves a promoting role in cell migration and invasion (31).…”