Long noncoding RNA SNHG12 promotes cell proliferation and activates Wnt/β‐catenin signaling in prostate cancer through sponging microRNA‐195

Abstract

Long noncoding RNAs (lncRNAs) serve critical roles in multiple human malignant tumors, including prostate cancer (PCa). Currently, the biological role of oncogenic lncRNA SNHG12 in PCa remains largely unclear. In the present study, we found that SNHG12 was highly expressed in human PCa tissues and cell lines. In addition, gain-of-function and loss-of-function studies showed that overexpression of SNHG12 promoted, while downregulation suppressed the proliferation, invasion, and migration of PCa cells in vitro.Knockdown of SNHG12 also repressed PCa xenograft tumor growth in vivo. Further in-depth mechanistic studies showed that SNHG12 might serve as a competing endogenous RNA for miR-195 in PCa cells, and miR-195 expression level was negatively associated with the expression of SNHG12 in PCa tissues.Finally, we found that the activity of Wnt/β-catenin signaling is enhanced by SNHG12 overexpression and rescued by co-transfection with miR-195 mimics in PCa cells. Collectively, the present study indicated the oncogenic function of SNHG12 in PCa and our findings might provide a new target in the treatment of PCa. K E Y W O R D S long noncoding RNA, microRNA-195, prostate cancer, SNHG12, Wnt/β-catenin signaling

Long noncoding RNA SNHG12 induces proliferation, migration, epithelial–mesenchymal transition, and stemness of esophageal squamous cell carcinoma cells via post‐transcriptional regulation of BMI1 and CTNNB1

“…Knockdown of lncRNA SNHG5 represses the development of glioma through Wnt/ CTNNB1 signaling pathway (Hu et al, 2019). Moreover, the interaction between SNHG12 and Wnt pathway has been researched in papillary thyroid carcinoma and prostate cancer (Ding et al, 2018;Song et al, 2019). Hence, it is worthy to explore the effects of SNHG12 on Wnt pathway downstream target in ESCC.…”

Biomarkers Associated With Metastasis and Prognosis of Lung Cancer Based on Microarray Databiomarkers Associated With Metastasis and Prognosis of Lung Cancer Based on Microarray Data

“…Recent evidences show that lncRNA SNHG12 promotes proliferation and metastasis of osteosarcoma and papillary thyroid carcinoma (34,35), which was consistent with our ndings. The oncogenic role of lncRNA SNHG12 is also determined in cervical cancer (36) and prostate cancer (37). However, the prognostic role in lung adenocarcinoma has been reported rarely.…”

Long Noncoding RNA SNHG12 is a Potential Diagnostic and Prognostic Biomarker in Various Tumors

“…Moreover, in lung cancer, this molecule can inhibit metastasis and epithelial-mesenchymal transition when inhibited [12] . Furthermore, increased SNHG12 not only regulates malignant behavior by activating oncogenes (AMOT, HuR slug/ZEB2) [12,33,34] , signaling pathways (MAPK/Slug, wnt/β-catenin, and Notch signaling pathway) [10,11,29,30,35] and the microRNA-gene axis (miR-125b/STAT3 and miR-101-3p/FOXP1) [27,31,36] but also enhances chemoresistance of tumor cells [31,32,37] . SNHG12 is negative regulation miR-195-5p, miR-320, miR-129, miR-193a-3p, microRNA-199a/b-5p and miR-424-5 [11,14,15,17,26] .…”