Long noncoding RNA SNHG12 promotes the proliferation, migration, and invasion of trophoblast cells by regulating the epithelial–mesenchymal transition and cell cycle

Abstract

Objective The deficient placental blood perfusion caused by the attenuated infiltration of trophoblast cells is a key factor in the occurrence of preeclampsia (PE). Furthermore, the long noncoding (lnc)RNA SNHG12 (small nucleolar RNA host gene 12) can promote the proliferation and metastasis of multiple tumor cells. However, whether lncRNA SNHG12 affects proliferation and metastasis of trophoblast cells is unclear. Methods We examined the level of lncRNA SNHG12 in plasma and placenta of patients with PE and constructed trophoblast cells with overexpressed or knocked down SNHG12. CCK-8, wound healing, and Transwell assays were used to detect alterations in proliferation, migration, and invasion of trophoblast cells. Western blotting was used to detect proteins related to the epithelial–mesenchymal transition (EMT), and cell cycle assays clarified cell cycle distribution. Results LncRNA SNHG12 promoted the proliferation, migration, and invasion of trophoblast cells. The expression of matrix metalloproteinase-2 (MMP-2) and MMP-9, β-catenin, and vimentin were positively correlated with SNHG12, and expression of E-cadherin was negatively correlated with SNHG12. SNHG12 also promoted the transition of trophoblast cells from G0/G1 to S phase. Conclusion Overall, lncRNA SNHG12 promoted the migration and invasion of trophoblast cells by inducing the progression of EMT.

Increased LINC00922 in preeclampsia regulates the proliferation, invasion, and migration of placental trophoblast cells

“…For example, lncRNA CCAT1 was highly expressed in PE, which could promote the progression of PE by inhibiting the expression of CDK4 (16). LncRNA SNHG12 promoted the transition of trophoblast cells from the G0/G1 phase to S phase, and then promoted the occurrence of PE (29). This study further showed that knockdown of LINC00922 significantly inhibited the expression of cell cycle-related proteins CDK2, Cyclin D1, and PCNA, and promoted the expression of p21, while overexpression of LINC00922 had the opposite effect.…”

The Gut Microbiota Dysbiosis in Preeclampsia Contributed to Trophoblast Cell Proliferation, Invasion, and Migration via lncRNA BC030099/NF-κB Pathway

“…Knockdown of lncRNA CRNDE has been reported to inhibit the proliferation, migration, and invasion of HTR-8/SVneo cells, inhibit the formation of EMT, and reduce the protein expression of MMP2 and MMP9 [31]. Zhou et al reported that lncRNA SNHG12 promoted trophoblast cell migration and invasion by inducing the progression of EMT [32]. Consistent with their study, we found knockdown of lncRNA BC030099 elevated MMP2, MMP9, and snail levels and repressed E-cadherin level.…”

Delayed puberty, gonadotropin abnormalities and subfertility in malePadi2/Padi4double knockout mice

“…Alternatively lncRNAs can directly interact with other chromatin modifiers to recruit or deflect their action on transcription (49,50). One lncRNA worth mentioning here, Snhg12 , has been linked to regulation of the cell cycle and promotion of the Epithelial-Mesenchymal Transition in embryogenesis (51). In addition, Snhg12 has been shown to promote cell proliferation and inhibit apoptosis in multiple cancers (52), including testosterone-sensitive prostate cancer (53).…”