Long noncoding RNA SBF2‐AS1 promotes colorectal cancer proliferation and invasion by inhibiting miR‐619‐5p activity and facilitating HDAC3 expression

Chen, Gang; Gu, Yue; Han, Peng; Zhong, Li; Zhao, Jinlu; Gao, Ming

doi:10.1002/jcp.28509

Cited by 41 publications

(31 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Accumulating evidence has suggested that various lncRNAs are involved in regulating the progression of colorectal cancer . However, we are still far from understanding the precise molecular mechanism of lncRNAs in colorectal cancer.…”

Section: Discussionmentioning

confidence: 99%

The long non‐coding RNA HCG18 promotes the growth and invasion of colorectal cancer cells through sponging miR‐1271 and upregulating MTDH/Wnt/β‐catenin

Zhang

et al. 2020

Clin Exp Pharma Physio

View full text Add to dashboard Cite

Long non‐coding RNAs (lncRNAs) have recently emerged as key regulators of the occurrence and progression of various human cancers, including colorectal cancer. However, the regulatory mechanism of lncRNAs in the tumorigenesis of colorectal cancer remains poorly understood. In this study, we aimed to elucidate the potential role of lncRNA HCG18 in colorectal cancer. Herein, we found that HCG18 expression was significantly upregulated in colorectal cancer tissues and cell lines. Knockdown of HCG18 significantly inhibited the growth and invasion of colorectal cancer cells, while its overexpression had the opposite effect. Moreover, HCG18 was identified as a sponge of miR‐1271. Our results showed that knockdown of HCG18 markedly upregulated miR‐1271 expression in colorectal cancer cells. Notably, HCG18 expression was inversely correlated with miR‐1271 expression in colorectal cancer specimens. Further investigation revealed that HCG18 contributed to the enhancement of MTDH/Wnt/β‐catenin signalling in colorectal cancer cells. The antitumour effect of HCG18 inhibition was significantly reversed by miR‐1271 inhibition or MTDH overexpression. Overall, the results of our study demonstrate that HCG18 exerts a potential oncogenic function in colorectal cancer by enhancing MTDH/Wnt/β‐catenin signalling via sponging of miR‐1271, highlighting the importance of HCG18/miR‐1271/ MTDH/Wnt/β‐catenin signalling in the progression of colorectal cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

The long non‐coding RNA HCG18 promotes the growth and invasion of colorectal cancer cells through sponging miR‐1271 and upregulating MTDH/Wnt/β‐catenin

Zhang

et al. 2020

Clin Exp Pharma Physio

View full text Add to dashboard Cite

show abstract

“…It has been well reported that lncRNAs play significant roles in cancer biology, including proliferation, invasion, apoptosis and metastasis ( 7 , 8 ). Aberrant expression of lncRNAs has been observed in the occurrence and development of a variety of cancers, suggesting that they may serve as powerful mediators in carcinogenesis ( 9 – 11 ). Over the past few decades, researchers have focused on investigating the function of lncRNAs in the pathogenesis of CRC ( 12 , 13 ).…”

Section: Introductionmentioning

confidence: 99%

Downregulation of LINC00958 inhibits proliferation, invasion and migration, and promotes apoptosis of colorectal cancer cells by targeting miR‑3619‑5p

et al. 2020

View full text Add to dashboard Cite

The aberrant expression of long non-coding RNAs (lncRNAs), including LINC00958, has been demonstrated in several types cancers. The present study aimed to investigate the role of LINC00958 in colorectal cancer (CRC) and identify the possible underlying mechanisms. The expression of LINC00958 and microRNA (miR)-3619-5p was detected in several human CRC cell lines using reverse transcription-quantitative PCR. Then, short hairpin RNA (shRNA)-LINC00958 was transfected into the cells. The results revealed that the expression of LINC00958 was notably upregulated, whereas miR-3619-5p was downregulated in CRC cells. Transfection with shRNA-LINC00958 inhibited the proliferation, invasion and migration of CRC cells. Moreover, the rate of apoptosis was enhanced, accompanied by a decrease in the expression of Bcl-2 and an increase in the expression of Bax and caspase-3. A luciferase reporter assay was conducted to verify the target binding site between LINC00958 and miR-3619-5p. The luciferase reporter assay confirmed that miR-3619-5p could be directly targeted by LINC00958. Furthermore, the miR-3619-5p inhibitor reversed the effects of LINC00958 silencing on proliferation, invasion, migration and apoptosis. Taken together, the findings suggest that the downregulation of LINC00958 suppresses the proliferation, invasion and migration, and promotes the apoptosis of CRC cells by targeting miR-3619-5p in vitro, which provides a theoretical basis and therapeutic strategy for the treatment of CRC.

show abstract

“…Bioinformatic analyses and luciferase reporter assays verified that miR-619-5p was the binding target of BCYRN1. MiR-619-5p has been reported to play important roles in several diseases [54,55]. For example, miR-619-5p was markedly downregulated in colorectal carcinoma and predicted to be a prognostic indicator of CRC patients [55].…”

Section: Discussionmentioning

confidence: 99%

LncRNA BCYRN1 inhibits glioma tumorigenesis by competitively binding with miR-619-5p to regulate CUEDC2 expression and the PTEN/AKT/p21 pathway

Niu

Zhang

et al. 2020

Oncogene

View full text Add to dashboard Cite

Glioma is the most common malignant tumor in the central nervous system. Altered long noncoding RNAs (lncRNAs) are playing regulatory roles in physiological and pathogenic processes in cancer. Here, we uncovered a differentially expressed lncRNA called brain cytoplasmic RNA 1 (BCYRN1), and elucidated its function and molecular mechanism in the progression and development of glioma. Three fresh tumor tissues from glioma patients and three normal brain tissues from craniocerebral trauma patients were prepared for high-throughput RNA sequencing. Differential RNA transcripts and BCYRN1 were identified by RT-qPCR in glioma samples and controls. CCK-8, colony formation assays, flow cytometry, TUNEL assays, cell migration assays, wound-healing assays, and xenograft model were established to investigate the biological function of BCYRN1 both in vitro and in vivo. Various bioinformatics analysis, dual-luciferase reporter assays, biotinylated RNA pulldown assays, and rescue experiments were conducted to reveal the underlying mechanisms of competitive endogenous RNAs (ceRNAs). 183 lncRNAs were identified with significant dysregulation in glioma and randomly selected differential RNAs were further confirmed by RT-qPCR. Among them, BCYRN1 was the most downregulated lncRNA, and its low expression positively correlated with glioma progression. Functionally, BCYRN1 overexpression inhibited cell proliferation, migration in glioma cell lines, whereas BCYRN1 depletion resulted in the opposite way. MiR-619-5p was further confirmed as the direct target of BCYRN1. Mechanistically, miR-619-5p specifically targeted the CUE domain containing protein 2 (CUEDC2), and BCYRN1/miR-619-5p suppressed glioma tumorigenesis by inactivating PTEN/AKT/p21 pathway in a CUEDC2-dependent manner. Overall, our data presented that the reduced expression of BCYRN1 was associated with poor patient outcome in glioma. BCYRN1 functioned as a ceRNA to inhibit glioma progression by sponging miR-619-5p to regulate CUEDC2 expression and PTEN/AKT/p21 pathway. Our results indicated that BCYRN1 exerted tumor suppressor potential and might be a candidate in the diagnosis and treatment of glioma.

show abstract

Long noncoding RNA SBF2‐AS1 promotes colorectal cancer proliferation and invasion by inhibiting miR‐619‐5p activity and facilitating HDAC3 expression

Cited by 41 publications

References 20 publications

The long non‐coding RNA HCG18 promotes the growth and invasion of colorectal cancer cells through sponging miR‐1271 and upregulating MTDH/Wnt/β‐catenin

The long non‐coding RNA HCG18 promotes the growth and invasion of colorectal cancer cells through sponging miR‐1271 and upregulating MTDH/Wnt/β‐catenin

Downregulation of LINC00958 inhibits proliferation, invasion and migration, and promotes apoptosis of colorectal cancer cells by targeting miR‑3619‑5p

LncRNA BCYRN1 inhibits glioma tumorigenesis by competitively binding with miR-619-5p to regulate CUEDC2 expression and the PTEN/AKT/p21 pathway

Contact Info

Product

Resources

About