Long Noncoding RNA RGMB-AS1 Indicates a Poor Prognosis and Modulates Cell Proliferation, Migration and Invasion in Lung Adenocarcinoma

Abstract

Lung cancer is the most common cause of cancer-related mortality worldwide. It is a complex disease involving multiple genetic and epigenetic alterations. The development of transcriptomics revealed the important role of long non-coding RNAs (lncRNAs) in lung cancer occurrence and development. Here, microarray analysis of lung adenocarcinoma tissues showed the abnormal expression of lncRNA RGMB-AS1. However, the role of lncRNA RGMB-AS1 in lung adenocarcinoma remains largely unknown. We showed that upregulation of lncRNA RGMB-AS1 was significantly correlated with differentiation, TNM stage, and lymph node metastasis. In lung adenocarcinoma cells, downregulation of lncRNA RGMB-AS1 inhibited cell proliferation, migration, invasion, and caused cell cycle arrest at the G1/G0 phase. In vivo experiments showed that lncRNA RGMB-AS1 downregulation significantly suppressed the growth of lung adenocarcinoma. The expression of lncRNA RGMB-AS1 was inversely correlated with that of repulsive guidance molecule b (RGMB) in lung adenocarcinoma tissues, and UCSC analysis and fluorescence detection assay indicated that lncRNA RGMB-AS1 may be involved in the development of human lung adenocarcinoma by regulating RGMB expression though exon2 of RGMB. In summary, our findings indicate that lncRNA RGMB-AS1 may play an important role in lung adenocarcinoma and may serve as a potential therapeutic target.

Circulating long noncoding RNA act as potential novel biomarkers for diagnosis and prognosis of non‐small cell lung cancer

“…Our study had three progressive stages: the screening stage, the training stage, and the validation stage. In the screening stage, we selected 14 lncRNA that are reportedly dysregulated in NSCLC tissue (Feng et al, 2015;Han et al, 2015;Hou et al, 2014;Li et al, 2016;Lu et al, 2013;Nie et al, 2015Nie et al, , 2016Qiu et al, 2014;Thai et al, 2013;Wang et al, 2015;Weber et al, 2013;Wu et al, 2015;Zhang et al, 2014b;Zhao et al, 2015a) and measured their expressions in 20 paired NSCLC and matched nontumorous tissues. Next, lncRNA with aberrant expression in the tissue samples thus identified were measured in 92 paired samples (46 NSCLCs and 46 healthy controls).…”

Long non-coding RNA XLOC_006390 promotes cervical cancer proliferation and metastasis through the regulation of SET domain containing 8

“…However, recently numerous studies indicate that lncRNAs contribute to different cellular processes (11)(12)(13). During the last decade, scientists have demonstrated that abnormal expression of lncRNAs is associated with human diseases (14)(15)(16), including different types of human cancers (17)(18)(19). The expression of lncRNA LINC01133 was decreased in human colorectal cancer (CRC) tissues and a low expression level of LIINC01133 was associated with poor survival in CRC patients.…”

A prognostic 10‐lncRNA expression signature for predicting the risk of tumour recurrence in breast cancer patients

“…RGMB-AS1 promoted cell proliferation, migration and invasion capabilities of lung cancer and thyroid papillary cancer. 35,36 RGMB-AS1 was down-regulated as an independent favourable prognostic factor for hepatocellular carcinoma patients. 37 The biological function of the remaining ln-cRNAs (TMEM161B-AS1, CADM3-AS1, LINC00293, LINC00910, LINC01187, PDZRN3-AS1 and ZBED5-AS1) in our signature has not been investigated in previous studies; thus, further studies are required to investigate the underlying molecular mechanisms of these diagnostic lncRNAs.…”

“…RGMB‐AS1 was reported to play important roles in lung cancer progression, the expression levels of which were significantly correlated with differentiation, TNM stage and lymph node metastasis. RGMB‐AS1 promoted cell proliferation, migration and invasion capabilities of lung cancer and thyroid papillary cancer . RGMB‐AS1 was down‐regulated as an independent favourable prognostic factor for hepatocellular carcinoma patients .…”