Long Noncoding RNA MHRT Protects Cardiomyocytes against H2O2-Induced Apoptosis

Zhang, Jianying; Gao, Caihua; Meng, Meijuan; Tang, Hongxia

doi:10.4062/biomolther.2015.066

Cited by 71 publications

(51 citation statements)

References 19 publications

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“…The lncRNA, autophagy promoting factor functioned to regulate autophagic cell death via targeting miR-188-3p and ATG7 [12]. Studies from Zhang et al, suggested that lncRNA, myosin heavy chain associated RNA transcripts (MHRT) is a protective factor for cardiomyocytes and the plasma concentration of MHRT may serve as a biomarker for myocardial infarction diagnosis in acute myocardial infarction [13]. The lncRNA, small nucleolar RNA host gene 1 (SNHG1) is a newly-identified lncRNA and has been found to modulate cancer progression in various types of cancers via different molecular mechanisms, and in vitro studies revealed that SNGH1 increased cell viability and inhibited cell apoptosis in the cancer cells [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

The Long Non-Coding RNA SNHG1 Attenuates Cell Apoptosis by Regulating miR-195 and BCL2-Like Protein 2 in Human Cardiomyocytes

Zhang

Meng

Mei

et al. 2018

Cell Physiol Biochem

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Background/Aims: Long non-coding RNAs (lncRNAs) are theorized to play key roles in the development of heart diseases. However, the role of lncRNAs in cardiomyocyte apoptosis is largely unknown. The present study examined the role of lncRNA SNHG1 in the human cardiomyocytes (HCMs) apoptosis and explored the underlying molecular mechanisms. Methods: SNHG1, miR-195 and mRNA expression was detected by qRT-PCR; protein level was determined by western blot; cell viability was detected by MTT assay; cell apoptosis was evaluated by flow cytometry and caspase-3 activity assay; the interaction between SNHG1 and miR195 was examined by using luciferase reporter assay. Results: Hydrogen peroxide (H₂O₂) treatment significantly suppressed cell viability and increased cell apoptotic rate and caspase-3 activity in HCMs. Overexpression of SNHG1 attenuated the effects of H₂O₂ on HCMs viability and apoptosis; while SNHG1 exerted the opposite effects. SNHG1 was found to sponge miR-195 and suppress the expression of miR-195 in HCMs. Overexpression of miR-195 suppressed cell viability and induced apoptosis in HCMs, and miR-195 was found to negatively regulate the expression of BCL-2 like protein 2 (BCL2L2) via targeting its 3’ untranslated region. Overexpression of BCL2L2 partially reversed the effects of miR-195 overexpression on cell viability and cell apoptosis of HCMs. MiR-195 overexpression or BCL2L2 knockdown attenuated the effects of SNHG1 overexpression on cell viability, cell apoptosis and protein levels of cleaved caspase-3, cleaved caspase-9 and Bax in H₂O₂-treated HCMs. Conclusion: Our results suggest a novel SNHG1/miR-195/BCL2L2 axis in the regulation of cardiomyocyte apoptosis. Modulation of SNHG1 may represent a novel strategy to treat cardiomyocyte apoptosis-related heart diseases.

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Section: Introductionmentioning

confidence: 99%

The Long Non-Coding RNA SNHG1 Attenuates Cell Apoptosis by Regulating miR-195 and BCL2-Like Protein 2 in Human Cardiomyocytes

Zhang

Meng

Mei

et al. 2018

Cell Physiol Biochem

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“…These RNAs have also been implicated in the diagnosis of cardiovascular diseases owing to the characteristic alterations of their circulating levels with different categories and grades of pathological processes. LncRNAs belong to a newly discovered class of functional mRNA‐like transcripts that lack significant open reading frames or protein‐coding capacity and have emerged as an important player in cardiovascular diseases, including a number of cardiac‐specific or cardiac‐related lncRNAs such as SRA , DIO3OS , SAF , NESPAS , MIAT , NRON , CARL , HCG22 , FENDRR , MHRT , aHIF , ZFAS1 and CDR1AS (http://cmbi.bjmu.edu.cn/lncrnadisease). Recent research data have also suggested the roles of lncRNAs in HF .…”

Section: Introductionmentioning

confidence: 99%

Circulating long non‐coding RNAs NRON and MHRT as novel predictive biomarkers of heart failure

Xuan

Sun

Zhang

et al. 2017

J Cellular Molecular Medi

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This study sought to evaluate the potential of circulating long non‐coding RNAs (lncRNAs) as biomarkers for heart failure (HF). We measured the circulating levels of 13 individual lncRNAs which are known to be relevant to cardiovascular disease in the plasma samples from 72 HF patients and 60 non‐HF control participants using real‐time reverse transcription‐polymerase chain reaction (real‐time RT‐PCR) methods. We found that out of the 13 lncRNAs tested, non‐coding repressor of NFAT (NRON) and myosin heavy‐chain‐associated RNA transcripts (MHRT) had significantly higher plasma levels in HF than in non‐HF subjects: 3.17 ± 0.30 versus 1.0 ± 0.07 for NRON (P < 0.0001) and 1.66 ± 0.14 versus 1.0 ± 0.12 for MHRT (P < 0.0001). The area under the ROC curve was 0.865 for NRON and 0.702 for MHRT. Univariate and multivariate analyses identified NRON and MHRT as independent predictors for HF. Spearman's rank correlation analysis showed that NRON was negatively correlated with HDL and positively correlated with LDH, whereas MHRT was positively correlated with AST and LDH. Hence, elevation of circulating NRON and MHRT predicts HF and may be considered as novel biomarkers of HF.

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“…MHRT blood levels were elevated in patients with acute myocardial infarction or heart failure. 18,19 We observed that MHRT expression levels in PBMCs are elevated in hypertensive patients compared to healthy controls.…”

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confidence: 82%

The long non‐coding RNAs MHRT,FENDRR and CARMEN, their expression levels in peripheral blood mononuclear cells in patients with essential hypertension and their relation to heart hypertrophy

Kontaraki

Marketou

Kochiadakis

et al. 2018

Clin Exp Pharma Physio

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Long non-coding RNAs (lncRNAs) participate in the modulation of cardiac hypertrophy, and they represent potential therapeutic targets in cardiovascular disease. We investigated the expression profiles of selected lncRNAs in peripheral blood mononuclear cells of patients with essential hypertension in relation to left ventricular hypertrophy. We assessed the expression levels of the lncRNAs MHRT, FENDRR and CARMEN using real-time reverse transcription polymerase chain reaction. Hypertensive patients showed significantly higher MHRT, FENDRR and CARMEN expression levels compared with healthy controls. In addition, we observed significant negative correlations of MHRT (r = -0.323, P = 0.003) and FENDRR (r = -0.380, P = 0.001) and a positive correlation of CARMEN (r = 0.458, P < 0.001) expression levels with left ventricular mass index. Our data reveal that the lncRNAs MHRT, FENDRR and CARMEN show distinct expression profiles in hypertensive patients and they possibly represent candidate therapeutic targets in hypertensive heart disease.

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Long Noncoding RNA MHRT Protects Cardiomyocytes against H2O2-Induced Apoptosis

Cited by 71 publications

References 19 publications

The Long Non-Coding RNA SNHG1 Attenuates Cell Apoptosis by Regulating miR-195 and BCL2-Like Protein 2 in Human Cardiomyocytes

The Long Non-Coding RNA SNHG1 Attenuates Cell Apoptosis by Regulating miR-195 and BCL2-Like Protein 2 in Human Cardiomyocytes

Circulating long non‐coding RNAs NRON and MHRT as novel predictive biomarkers of heart failure

The long non‐coding RNAs MHRT,FENDRR and CARMEN, their expression levels in peripheral blood mononuclear cells in patients with essential hypertension and their relation to heart hypertrophy

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