Long noncoding RNA MAFG‑AS1 promotes proliferation, migration and invasion of hepatocellular carcinoma cells through downregulation of miR‑6852

Ouyang, Hui; Zhang, Li; Xie, Zhen; Ma, Simin

doi:10.3892/etm.2019.7850

Cited by 18 publications

(25 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, our func- tional experiments validated that compared with the control group, MAFG-AS1 silencing could significantly repress the proliferation, migration, and invasion of BLCA cells. The data suggest that, in BLCA, MAFG-AS1 exerts oncogenic function and is a promising therapy target, which is consistent with previous reports [10,11,[25][26][27]. miRNAs are endogenous small noncoding RNAs containing 18-25 nucleotides.…”

Section: Discussionsupporting

confidence: 90%

“…According to previous reports, MAFG-AS1 is a cancerpromoting lncRNA that is involved in non-small cell carcinoma, hepatocellular carcinoma, gastric cancer, breast cancer, and colorectal cancer [10,11,[25][26][27]. It has also been reported that the upregulation of MAFG-AS1 in lung adenocarcinoma tissues is associated with adverse prognosis of patients.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

LncRNA MAFG-AS1 Promotes the Progression of Bladder Cancer by Targeting the miR-143-3p/COX-2 Axis

Zhong

Zhu

et al. 2020

Pathobiology

View full text Add to dashboard Cite

Background: Long noncoding RNAs (lncRNAs) are potential biomarkers that are very important for the development of cancer. Studies show that lncRNAs are significantly correlated with the carcinogenesis and progression of bladder cancer (BLCA). In this research, we aimed at probing into the role of lncRNA MAFG-AS1 in the tumorigenesis of BLCA. Methods: RT-qPCR was employed to detect MAFG-AS1 expression in BLCA tissues and cells. MAFG-AS1 siRNA and overexpression plasmid were transfected into 5637 and T24 BLCA cell lines to inhibit or upregulate MAFG-AS1 expression, respectively, and then the regulatory functions of MAFG-AS1 on BLCA cell proliferation, migration, and invasion were assessed using cell counting kit-8 (CCK-8) assay, EdU method, and Transwell experiments, respectively. Dual-luciferase reporter assay and RNA immunoprecipitation were conducted to validate the targeting relationships between MAFG-AS1 and miR-143-3p, and miR-143-3p and COX-2. In addition, miR-143-3p was repressed in MAFG-AS1-silenced 5637 and T24 cell lines, and the function of MAFG-AS1/miR-143-3p axis in BLCA cells was further evaluated. The regulatory effects of MAFG-AS1 and miR-143-3p on the expression of COX-2 protein were detected by Western blot. Results: MAFG-AS1 was remarkably upregulated in BLCA patient tissues and cell lines, and its high expression was closely related to histological grade, tumor size, and lymph node metastasis. Silencing of MAFG-AS1 inhibited BLCA cell proliferation, metastasis, and invasion, while overexpression of MAFG-AS1 in BLCA cells had opposite biological effects. MAFG-AS1 was proved to target miR-143-3p to repress its expression. Moreover, it was confirmed that MAFG-AS1 and miR-143-3p could modulate COX-2 expression. Conclusion: The MAFG-AS1/miR-143-3p/COX-2 axis contributes to BLCA progression.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

LncRNA MAFG-AS1 Promotes the Progression of Bladder Cancer by Targeting the miR-143-3p/COX-2 Axis

Zhong

Zhu

et al. 2020

Pathobiology

View full text Add to dashboard Cite

show abstract

“…Five fields (× 200) were randomly selected under the inverted microscope (XDS-800D, Caikon Optical Instrument Co., Ltd., Shanghai, China). The number of cells that crossed the chamber membrane was counted [ 7 ].…”

Section: Methodsmentioning

confidence: 99%

“…Long non-coding RNAs (lncRNAs), a group of non-coding RNA longer than 200 nucleotides, have attracted research attention considering their roles as oncogenes or anti-tumor genes in the pathogenesis of CRC for diagnosis methods and treatment modalities [ 5 , 6 ]. LncRNA MAF BZIP Transcription Factor G Antisense RNA 1 (MAFG-AS1) has been reported to function in multiple types of cancer, including hepatocellular carcinoma, lung adenocarcinoma, breast cancer, and non-small cell lung cancer through interplay with different microRNAs (miRNAs) [ 7 – 10 ]. To our best knowledge, there is only one report discussing the action of lncRNA MAFG-AS1 in CRC, where Cui et al .…”

Section: Introductionmentioning

confidence: 99%

Downregulation of long non-coding RNA MAFG-AS1 represses tumorigenesis of colorectal cancer cells through the microRNA-149-3p-dependent inhibition of HOXB8

et al. 2020

View full text Add to dashboard Cite

Background Colorectal cancer (CRC) is considered as the second common death-induced cancer. More recently, association of long non-coding RNAs (lncRNAs) with CRC has been extensively investigated. Therefore, the present study was performed to determine whether lncRNA MAF BZIP Transcription Factor G Antisense RNA 1 (MAFG-AS1) could regulate biological activities of CRC cells and unravel the underlying mechanisms. Methods CRC and corresponding adjacent tissues were collected to determine the expression of lncRNA MAFG-AS1, microRNA-149-3p (miR-149-3p) and homeobox B8 (HOXB8) by RT-qPCR. Dual luciferase reporter gene assay was used to explore the targeting relationship between miR-149-3p and lncRNA MAFG-AS1 and between miR-149-3p and HOXB8, followed by RNA immunoprecipitation for verification. Migration, proliferation, invasion, and apoptosis of HCT116 and LoVo cells were examined when lncRNA MAFG-AS1 was silenced or miR-149-3p was overexpressed. Furthermore, tumorigenicity of HCT116 and LoVo cells was measured in vivo by tumor xenograft in nude mice. Results LncRNA MAFG-AS1 and HOXB8 were found to be highly expressed in CRC tissues and cells, while miR-149-3p was under-expressed. LncRNA MAFG-AS1 negatively regulated miR-149-3p while miR-149-3p downregulated HOXB8. In addition, lncRNA MAFG-AS1 silencing by shRNA or miR-149-3p upregulation by mimic suppressed the migration, proliferation, invasion and tumorigenesis but promoted the apoptosis of HCT116 and LoVo cells. Conclusion Taken together, lncRNA MAFG-AS1 downregulation inhibits the malignant behaviors of CRC cells by upregulating miR-149-3p and downregulating HOXB8, providing a potential therapeutic target for CRC treatment.

show abstract

“…. A recent study by Ouyang et al 24 indicated that the levels of MAFG-AS1 were increased in hepatocellular carcinoma specimens and cell lines. In their in vitro assays, the knockdown of MAFG-AS1 was demonstrated to inhibit the proliferation and metastasis of hepatocellular carcinoma cells via sponging miRNA-6852.…”

Section: Mmp15 Axis 18mentioning

confidence: 98%

High-expression of LncRNA MAFG-AS1 is associated with the prognostic of patients with colorectal cancer

Cui

Wang

Shen³

et al. 2020

Rev. Assoc. Med. Bras.

View full text Add to dashboard Cite

SUMMARY OBJECTIVE: Long noncoding RNAs (lncRNAs) have been proven to exhibit distinct functions on the convoluted processes of tumor developments. Some studies on the biological functions of lncRNA MAFG-AS1 (MAFG-AS1) in cancers revealed that they may serve as an oncogene in some kinds of tumors, including colorectal cancer (CRC). However, little is known about the role of MAFG-AS1 in the prognostic of CRC. METHODS: A public dataset was mined for the screening of dysregulated lncRNAs in CRC. Quantitative real-time reverse transcription-polymerase chain reaction(qPCR) was used to compare the levels of MAFG-AS1 between paired MAFG-AS1 specimens and normal adjacent tissues. The correlations between MAFG-AS1 and clinic pathological features in CRC were analyzed using the chi-square test. The log-rank test and Kaplan-Meier test were carried out to compare the survival time of patients with high and low expressions of MAFG-AS1. Cox regression was applied for univariate and multivariate assays to validate whether MAFG-AS1 could be an independent factor in the prognosis of CRC. RESULTS: We found that the distinct upregulation of MAFG-AS1 in various tumors was a common event. MAFG-AS1 was distinctly up-regulated in CRC specimens compared to matched non-tumor specimens (p < 0.01). High MAFG-AS1 expressions were closely associated with depth of invasion (p = 0.011) and TNM stage (p = 0.022). Survival assays revealed that patients with high expression of MAFG-AS1 have a shorter overall survival (p = 0.0030) and disease-free survival (p = 0.0002). CONCLUSIONS: MAFG-AS1 can serve as a novel potential biomarker to predict CRC patients’ survival time.

show abstract

Long noncoding RNA MAFG‑AS1 promotes proliferation, migration and invasion of hepatocellular carcinoma cells through downregulation of miR‑6852

Cited by 18 publications

References 27 publications

LncRNA MAFG-AS1 Promotes the Progression of Bladder Cancer by Targeting the miR-143-3p/COX-2 Axis

LncRNA MAFG-AS1 Promotes the Progression of Bladder Cancer by Targeting the miR-143-3p/COX-2 Axis

Downregulation of long non-coding RNA MAFG-AS1 represses tumorigenesis of colorectal cancer cells through the microRNA-149-3p-dependent inhibition of HOXB8

High-expression of LncRNA MAFG-AS1 is associated with the prognostic of patients with colorectal cancer

Contact Info

Product

Resources

About