Long noncoding RNA MACC1-AS1 promotes the stemness of hepatocellular carcinoma cells by antagonizing miR-145

Guo, Yi; Zhong, Jiuhong; Wu, Fang; Zhan, Zhi-Hui

doi:10.1177/0300060520920411

Cited by 9 publications

(7 citation statements)

References 20 publications

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“…Some lncRNAs were confirmed to be aberrantly expressed and to participate in the pathophysiology of ischemic stroke (12,13), and in the present study, lncRNA MACC1-AS1 was found to be downregulated in hypoxia-induced vascular endothelial cells. LncRNA MACC1-AS1 has been identified as an oncogene in hepatic carcinoma, lung adenocarcinoma, pancreatic carcinoma, gastric cancer, and other cancers (19,(28)(29)(30), but its role in other diseases remains relatively unexplored.…”

Section: Discussionmentioning

confidence: 99%

LncRNA MACC1-AS1 attenuates microvascular endothelial cell injury and promotes angiogenesis under hypoxic conditions via modulating miR-6867-5p/TWIST1 in human brain microvascular endothelial cells

Yan¹,

Zhao²,

Xiao³

2020

Ann Transl Med

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Background: Hypoxia following ischemic stroke is a common cause of brain insults. Mounting evidence suggests that long non-coding RNAs (lncRNAs) play a vital role in regulating certain physiological and pathological processes including ischemic stroke. For the first time, the present study investigated the effects and mechanism of LncRNA MACC1-AS1 on hypoxia-induced human brain microvascular endothelial cells (HBMECs).Methods: LncRNA MACC1-AS1 levels in HBMECs were detected via reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay. Reactive oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT), were detected using their respective kits. Flow cytometry and clone formation assay were performed to evaluate the effects of lncRNA MACC1-AS1 on cell apoptosis and cell proliferation respectively. Angiogenesis capacity was evaluated via tube formation assay. Transwell migration assay was performed for assessment of cell migration, Western blot assay was performed for measurement of Twist1 and VE-cadherin level, and permeability assay was performed for evaluation of the cell barrier function. The target gene was predicted via bioinformatics online tool and validated through luciferase reporter assay and RNA pull-down assay.Results: LncRNA MACC1-AS1 was downregulated in hypoxia-induced HBMECs. Overexpression of LncRNA MACC1-AS1 reduced cell apoptosis and oxidative stress, while promoting cell proliferation, migration, and angiogenesis. Moreover, LncRNA MACC1-AS1 overexpression reduced cell permeability and elevated VE-cadherin level, which contributed to maintaining cell barrier function. TWIST1 was validated as the target of miR-6867-5p which was further targeted by lncRNA MACC1-AS1. Thus, LncRNA MACC1-AS1 functions in hypoxia-induced HBMECs by regulating miR-6867-5p/TWIST1. Conclusions:In this study, we found that LncRNA MACC1-AS1 exerted a protective role in hypoxiainduced HBMECs via regulating miR-6867-5p/TWIST1, indicating a new therapeutic strategy for future ischemic stroke therapy.

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Section: Discussionmentioning

confidence: 99%

LncRNA MACC1-AS1 attenuates microvascular endothelial cell injury and promotes angiogenesis under hypoxic conditions via modulating miR-6867-5p/TWIST1 in human brain microvascular endothelial cells

Yan¹,

Zhao²,

Xiao³

2020

Ann Transl Med

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“…MACC1-AS1, a homologous antisense lncRNA for the sixth intron of the MACC1 gene, has been linked to the development of certain malignancies through influencing miRNAs, according to numerous studies. MACC1-AS1 promotes proliferation by downregulating PTEN in lung adenocarcinoma cells [ 97 ], promoting invasion and proliferation by regulating paired-box gene 8 (PAX8) in hepatoma cells [ 98 ], promoting stemness by antagonizing miR-145 [ 99 ], and upregulating cyclin-dependent kinase 6 in cervical squamous cell carcinoma [ 100 ]. In pancreatic cancer, it promotes cancer progression by activating the PAX8/NOTCH1 signaling pathway [ 101 ].…”

Section: Macc1-as1 Participates In Bc’s Cerna Regulatory Networkmentioning

confidence: 99%

MACC1 as a Potential Target for the Treatment and Prevention of Breast Cancer

Jiao

Yang

et al. 2023

Biology

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Metastasis associated in colon cancer 1 (MACC1) is an oncogene first identified in colon cancer. MACC1 has been identified in more than 20 different types of solid cancers. It is a key prognostic biomarker in clinical practice and is involved in recurrence, metastasis, and survival in many types of human cancers. MACC1 is significantly associated with the primary tumor, lymph node metastasis, distant metastasis classification, and clinical staging in patients with breast cancer (BC), and MACC1 overexpression is associated with reduced recurrence-free survival (RFS) and worse overall survival (OS) in patients. In addition, MACC1 is involved in BC progression in multiple ways. MACC1 promotes the immune escape of BC cells by affecting the infiltration of immune cells in the tumor microenvironment. Since the FGD5AS1/miR-497/MACC1 axis inhibits the apoptotic pathway in radiation-resistant BC tissues and cell lines, the MACC1 gene may play an important role in BC resistance to radiation. Since MACC1 is involved in numerous biological processes inside and outside BC cells, it is a key player in the tumor microenvironment. Focusing on MACC1, this article briefly discusses its biological effects, emphasizes its molecular mechanisms and pathways of action, and describes its use in the treatment and prevention of breast cancer.

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“…LncRNA LINC00460 regulates liver cancer cell proliferation by targeting the miR-503-5p/miR-654-3p/TCP1 axis ( 118 ). Long noncoding RNA MACC1-AS1 promotes ALDH1 in stem cells from hepatocellular carcinoma by antagonizing miR-145 ( 119 ). Carboxypeptidase A4 upregulates ALDH1 expression and promotes proliferation of hepatocellular carcinoma ( 120 ).…”

Section: Aldh1 and Various Solid Tumorsmentioning

confidence: 99%

ALDH1: A potential therapeutic target for cancer stem cells in solid tumors

Wei

Chen

et al. 2022

Front. Oncol.

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Solid tumors can be divided into benign solid tumors and solid malignant tumors in the academic community, among which malignant solid tumors are called cancers. Cancer is the second leading cause of death in the world, and the global incidence of cancer is increasing yearly New cancer patients in China are always the first. After the concept of stem cells was introduced in the tumor community, the CSC markers represented by ALDH1 have been widely studied due to their strong CSC cell characteristics and potential to be the driving force of tumor metastasis. In the research results in the past five years, it has been found that ALDH1 is highly expressed in various solid cancers such as breast cancer, lung cancer, colorectal cancer, liver cancer, gastric cancer, cervical cancer, esophageal cancer, ovarian cancer, head,and neck cancer. ALDH1 can activate and transform various pathways (such as the USP28/MYC signaling pathway, ALDH1A1/HIF-1α/VEGF axis, wnt/β-catenin signaling pathway), as well as change the intracellular pH value to promote formation and maintenance, resulting in drug resistance in tumors. By targeting and inhibiting ALDH1 in tumor stem cells, it can enhance the sensitivity of drugs and inhibit the proliferation, differentiation, and metastasis of solid tumor stem cells to some extent. This review discusses the relationship and pathway of ALDH1 with various solid tumors. It proposes that ALDH1 may serve as a diagnosis and therapeutic target for CSC, providing new insights and new strategies for reliable tumor treatment.

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Long noncoding RNA MACC1-AS1 promotes the stemness of hepatocellular carcinoma cells by antagonizing miR-145

Cited by 9 publications

References 20 publications

LncRNA MACC1-AS1 attenuates microvascular endothelial cell injury and promotes angiogenesis under hypoxic conditions via modulating miR-6867-5p/TWIST1 in human brain microvascular endothelial cells

LncRNA MACC1-AS1 attenuates microvascular endothelial cell injury and promotes angiogenesis under hypoxic conditions via modulating miR-6867-5p/TWIST1 in human brain microvascular endothelial cells

MACC1 as a Potential Target for the Treatment and Prevention of Breast Cancer

ALDH1: A potential therapeutic target for cancer stem cells in solid tumors

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