Long noncoding RNA lnc-sox5 modulates CRC tumorigenesis by unbalancing tumor microenvironment

Abstract

Long non-coding RNAs (LncRNAs) have been recently regarded as systemic regulators in multiple biologic processes including tumorigenesis. In this study, we observed the expression of lncRNA lnc-sox5 was significantly increased in colorectal cancer (CRC). Despite the CRC cell growth, cell cycle and cell apoptosis was not affected by lnc-sox5 knock-down, lnc-sox5 knock-down suppressed CRC cell migration and invasion. In addition, xenograft animal model suggested that lnc-sox5 knock-down significantly suppressed the CRC tumorigenesis. Our results also showed that the expression of indoleamine 2,3-dioxygenase 1 (IDO1) was significantly reduced by lnc-sox5 knock-down and therefore modulated the infiltration and cytotoxicity of CD3CD8T cells. Taken together, these results suggested that lnc-sox5 unbalances tumor microenvironment to regulate colorectal cancer progression.

Long Non-coding RNAs: Emerging Roles in the Immunosuppressive Tumor Microenvironment

“…Wu et al reported that lnc-sox5 was upregulated during the tumorigenesis of colorectal cancer (CRC). Additionally, the absence of lnc-sox5 did not affect the growth of tumor cells in immunodeficient mice, but significantly suppressed tumorigenesis in immunocompetent mice (50). Flow cytometry analysis suggested that the knock down of lnc-sox5 promoted the infiltration and the cytotoxicity of CD3 + CD8 + CTLs in tumors in immunocompetent mice.…”

Roles of circRNAs in the tumour microenvironment

“…Extensive and in-depth research on the TME should importantly involve noncoding RNAs (ncRNAs), such as microRNAs (miRNAs) [10][11][12] and long ncRNAs (lncRNAs) [13][14][15]. With the broad application of highthroughput RNA sequencing (RNA-seq), numerous circular RNAs (circRNAs) have been identified and characterized in humans and other eukaryotes.…”

Long non-coding RNA LINC00959 predicts colorectal cancer patient prognosis and inhibits tumor progression

“…Multiple lncRNAs have been identified as CRC oncogenes or tumor suppressors [ 23 , 24 , 25 ]. FEZF1-AS1 downregulation inhibits cell proliferation, migration, and invasion, suggesting that this lncRNA promotes CRC tumorigenesis and progression [ 26 ].…”