Long noncoding RNA lnc-RI regulates DNA damage repair and radiation sensitivity of CRC cells through NHEJ pathway

Liu, Ruixue; Zhang, Qingtong; Shen, Liping; Chen, Shuangjing; He, Junyan; Wang, Dong; Wang, Qi; Qi, Zhenhua; Zhou, Meijuan; Wang, Zhidong

doi:10.1007/s10565-020-09524-6

Cited by 27 publications

(19 citation statements)

References 45 publications

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“…Among them, lncRNAs are a class of RNAs > 200 nucleotides long that do not code for proteins [3,4]. Dysregulation of lncRNAs has been shown to be involved in physiological processes such as the regulation of gene expression [5], chromatin remodeling [6], maintenance of pluripotency [7], DNA damage repair [8], competing endogenous RNAs [9,10], and it also can participate in some pathological processes such as tumorigenesis [11].…”

Section: Introductionmentioning

confidence: 99%

Emerging role of long noncoding RNA-encoded micropeptides in cancer

Ye¹,

Zhang²,

Wang³

et al. 2020

Cancer Cell Int

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Increasing evidence has indicated that long noncoding RNAs (lncRNAs) play various important roles in the development of cancers. The widespread applications of ribosome profiling and ribosome nascent chain complex sequencing revealed that some short open reading frames of lncRNAs have micropeptide-coding potential. The resulting micropeptides have been shown to participate in N6-methyladenosine modification, tumor angiogenesis, cancer metabolism, and signal transduction. This review summarizes current information regarding the reported roles of lncRNA-encoded micropeptides in cancer, and explores the potential clinical value of these micropeptides in the development of anti-cancer drugs and prognostic tumor biomarkers.

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Section: Introductionmentioning

confidence: 99%

Emerging role of long noncoding RNA-encoded micropeptides in cancer

Ye¹,

Zhang²,

Wang³

et al. 2020

Cancer Cell Int

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“…5 LncRNA RI silencing increases radiosensitivity of colorectal cancer (CRC). 28 Zhang et al have demonstrated that PCAT1 knockdown strengthens the promoting effect of radiation on apoptosis of glioma stem cells. 8 Similarly, the current study showed that silencing of PCAT1 markedly enhanced the inhibiting effect of radiation therapy on CC cells.…”

Section: Discussionmentioning

confidence: 99%

<p>Knockdown of lncRNA PCAT1 Enhances Radiosensitivity of Cervical Cancer by Regulating miR-128/GOLM1 Axis</p>

et al. 2020

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Purpose: Cervical cancer (CC) is the fourth most common cancer with high death rate in females. The study aims to detect the mechanism of long non-coding RNA (LncRNA) PCAT1 on radiosensitivity of CC. Methods: The expression of PCAT1, miR-128 and GOLM1 in CC tissues and cells was measured by qRT-PCR. Different doses of X-ray were used for radiation treatment of CC cells and 6 Gy was chosen to perform the following experiments. The proliferation, migration and invasion of CC cells were measured by MTT assay, wound healing assay and transwell assay, respectively. The target relationships among PCAT1, miR-128 and GOLM1 were predicted by StarBase and TargetScan and verified by luciferase reporter assay. The protein level of GOLM1 was determined by Western blot. The xenograft tumor model was constructed in nude mice to verify the effect of PCAT1 on radiosensitivity of CC in vivo. Results: The PCAT1 expression was upregulated in CC tissues and cells. PCAT1 silencing enhances radiosensitivity of CC cells on proliferation, migration and invasion. MiR-128 was the target of PCAT1 and was negatively regulated by PCAT1. Upregulation of miR-128 enhances radiosensitivity of CC cells on proliferation, migration and invasion. GOLM1 was a target of miR-128 and was negatively regulated by miR-128. Upregulation of GOLM1 and downregulation of miR-128 both reversed the enhanced effect of PCAT1 knockdown on radiosensitivity of CC cells, which partly promoted the proliferation, migration and invasion of CC cells. Conclusion: Silencing of PCAT1 enhanced radiosensitivity of CC via targeting miR-128/ GOLM1, which provided a new idea for treating CC.

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“…miR-124, miR-502 and miR-545 regulate Ku70 [144][145][146], miR-526b, miR-622 and miR-218-5p control the level of Ku80 [89,147,148], miR-34a suppresses the expression of 53BP1 [149]. For some factors, in addition to miRNA, competing endogenous RNAs (ceRNA) have been described that are able to counteract the effects of specific miRNAs by direct binding (Figure 5) [150]; for example, miR-4727-5p was shown to inhibit the expression of DNA ligase IV, while aforementioned ceRNA lnc-RI, reversed this effect [151]. In addition, for miR-151a-3p, which inhibits XRCC4 expression, it was shown that ceRNA SBF2-AS1 neutralizes the effect of this miRNA [152].…”

Section: Different Types Of Rnas Are Involved In All Stages Of Nonhommentioning

confidence: 99%

The Role of RNA in DNA Breaks, Repair and Chromosomal Rearrangements

et al. 2021

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Incorrect reparation of DNA double-strand breaks (DSB) leading to chromosomal rearrangements is one of oncogenesis’s primary causes. Recently published data elucidate the key role of various types of RNA in DSB formation, recognition and repair. With growing interest in RNA biology, increasing RNAs are classified as crucial at the different stages of the main pathways of DSB repair in eukaryotic cells: nonhomologous end joining (NHEJ) and homology-directed repair (HDR). Gene mutations or variation in expression levels of such RNAs can lead to local DNA repair defects, increasing the chromosome aberration frequency. Moreover, it was demonstrated that some RNAs could stimulate long-range chromosomal rearrangements. In this review, we discuss recent evidence demonstrating the role of various RNAs in DSB formation and repair. We also consider how RNA may mediate certain chromosomal rearrangements in a sequence-specific manner.

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Long noncoding RNA lnc-RI regulates DNA damage repair and radiation sensitivity of CRC cells through NHEJ pathway

Cited by 27 publications

References 45 publications

Emerging role of long noncoding RNA-encoded micropeptides in cancer

Emerging role of long noncoding RNA-encoded micropeptides in cancer

<p>Knockdown of lncRNA PCAT1 Enhances Radiosensitivity of Cervical Cancer by Regulating miR-128/GOLM1 Axis</p>

The Role of RNA in DNA Breaks, Repair and Chromosomal Rearrangements

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