Long noncoding RNA LINC01123 promotes the proliferation and invasion of hepatocellular carcinoma cells by modulating the miR-34a-5p/TUFT1 axis

Abstract

Hepatocellular carcinoma (HCC), one of the main causes of cancer-related deaths globally, is characterized by rapid growth and high invasiveness. Accumulating evidence demonstrates that long noncoding RNAs (lncRNAs) play critical roles in the growth and metastasis of HCC. Recently, lncRNA LINC01123 has been found to contribute to cell proliferation and aerobic glycolysis in lung cancer. However, the function of LINC01123 in HCC, as well as the underlying mechanism of its action, remain unclear. Here, we found that the expression of LINC01123 was clearly upregulated in HCC tissues compared to nontumor tissues. Furthermore, expression of LINC01123 in HCC cells was significantly higher than in LO2 cells. Importantly, the upregulated level of LINC01123 was related to unfavorable clinical features and poor prognosis of HCC. Next, we demonstrated that LINC01123 knockdown suppressed the proliferation, migration and invasion of HCC cells in vitro . Depletion of LINC01123 inhibited HCC xenograft growth in vivo . Conversely, ectopic expression of LINC01123 facilitated HCC cell proliferation and invasion. Mechanistically, LINC01123 acted as a molecular sponge for miR-34a-5p in HCC cells. Tuftelin1 (TUFT1) was identified as the target gene of miR-34a-5p. LINC01123 positively regulated TUFT1 level by targeting of miR-34a-5p in HCC cells. Notably, TUFT1 restoration can abolish miR-34a-5p-induced inhibitory effects on HCC cell proliferation, migration and invasion. In conclusion, LINC01123 was overexpressed in HCC and accelerated cancer cell proliferation and invasion by regulating the miR-34a-5p/TUFT1 axis.

Long noncoding RNA FIRRE contributes to the proliferation and glycolysis of hepatocellular carcinoma cells by enhancing PFKFB4 expression

“…The dysregulation and dysfunction of lncRNAs participate in the initiation and progression of HCC [25]. For example, lncRNA LINC01123 is overexpressed in HCC, and its overexpression predicts poor prognosis of patients [16]. LINC01123 facilitates the proliferation, migration, and invasion of HCC cells via miR-34a-5p/TUFT1 pathway [16].…”

“…For example, lncRNA LINC01123 is overexpressed in HCC, and its overexpression predicts poor prognosis of patients [16]. LINC01123 facilitates the proliferation, migration, and invasion of HCC cells via miR-34a-5p/TUFT1 pathway [16]. LncRNA A1BG-AS1 is a tumor-suppressive factor in HCC and functions as a miR-216a-5p sponge to suppress cancer cells' proliferation and invasion [26].…”

“…The viability of HCC cells was detected using the Cell Counting Kit-8 (CCK-8) assay, according to the previously described protocol [16]. We used a microplate reader (Multiskan™ FC, Thermo Fisher Scientific) to measure the absorbance at 450 nm.…”

“…We used a microplate reader (Multiskan™ FC, Thermo Fisher Scientific) to measure the absorbance at 450 nm. The proliferation of HCC cells was detected by the 5-ethynyl-2′-deoxyuridine (EdU) assay using the Cell-Light™ EdU Apollo®488 In vitro Imaging Kit (RIBOBIO, Guangzhou, China) as previously described [16].…”

Long noncoding RNA LINC01123 promotes the proliferation and invasion of hepatocellular carcinoma cells by modulating the miR-34a-5p/TUFT1 axis: Erratum

“…In our paper 1 , the image of invaded Hep3B cells with empty vector transfection in Figure 3D was mis-pasted. Through repeated confirmation, we captured the images of invaded Hep3B cells with control siRNA or empty vector transfection at the same time and confirmed that we attached the same image of invaded Hep3B cells (control group) in Figure 2D and Figure 3D due to the negligence.…”