Long Noncoding RNA HCG11 Acts as a Tumor Suppressor in Gastric Cancer by Regulating miR-942-5p/BRMS1 Axis

Abstract

The functions of long noncoding RNAs (lncRNAs) have been widely investigated in human cancers, including gastric cancer (GC). The purpose of this study was to elucidate the role of lncRNA HCG11 in GC. In this study, mRNA and protein expressions were detected by quantitative real-time polymerase chain reaction assays (RT-qPCR) and Western blot analysis. The proliferation ability of GC cells was examined by (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyl Tetrazolium Bromide) MTT assays. The invasion and migration abilities of GC cells were evaluated by Transwell assays. The binding sites between miR-942-5p and HCG11/BRMS1 were confirmed by dual-luciferase reporter assays. Results showed that LncRNA HCG11 was downregulated in GC cells. Functionally, overexpression of HCG11 inhibited GC cell proliferation, migration, and invasion. In addition, lncRNA HCG11 was found to act as a molecular sponge of miR-942-5p. Furthermore, miR-942-5p promoted GC progression by suppressing lncRNA HCG11 expression. Besides that, BRMS1 was confirmed as a direct target of miR-942-5p. More importantly, breast cancer metastasis suppressor 1 (BRMS1) inhibited GC progression by upregulating lncRNA HCG11 and downregulating miR-942-5p. In conclusion, LncRNA HCG11 inhibited cell proliferation, migration, and invasion in GC by sponging miR-942-5p and upregulating BRMS1.

CircGSAP regulates the cell cycle of pulmonary microvascular endothelial cells via the miR-942-5p sponge in pulmonary hypertension

“…More importantly, we verified that miR-942-5p is involved in regulating the proliferation and apoptosis of PMECs. Many studies have shown that miR-942-5p regulates cell proliferation and disease progression in different types of tumors ( Li et al, 2020 ; Wang et al, 2020 ; Gu et al, 2021 ; Zhang et al, 2021 ); these findings are consistent with our results, because PMECs dysfunction in PH is a cancer cell-like phenotype. However, Wang et al found that miR-942-5p could inhibit PASMCs proliferation in vitro ( Wang et al, 2021 ), which is inconsistent with our results; a possible reason for this is the difference in the cell types studied and the cell culture conditions.…”

“…In those miRNAs, qRT-PCR showed that miR-942-5p was upregulated in hypoxia-induced PMECs, and diminished after overexpression of circGSAP levels ( Figures 3A,B ), which is consistent with the miRNA acts as circRNA sponge to influence the occurrence and development of diseases. and miR-942-5p regulates cell proliferation had been reported ( Li et al, 2020 ; Wang et al, 2020 ; Gu et al, 2021 ; Zhang et al, 2021 ), so, we speculate that miR-942-5p may be downstream of circGSAP, which affecting the function of PMECs. Then, potential binding sites between circGSAP and miR-942-5p were predicted using Circular RNA Interactome, and the representative putative target sites of miR-942-5p are shown in Figure 3C .…”

lncRNA HCG11 regulates the expression level of SP1 as a potential prognostic biomarker of BC during the polymerase II transcription process in an HCG11/miR-106a-3p/SP1 axis: integrated bioinformatics and experimental investigation

“…By altering miR-144-3p/PBX3 axis, lncRNA HCG11 knockdown slows the growth of ovarian cancer cells (41). lncRNA HCG11 controls the miR-942-5p/BRMS1 axis to act as a tumor suppressor in GC (42). By increasing SOCS5, the long non-coding RNA HCG11 sponging miR-522-3p prevents the growth of non-small cell lung cancer (43).…”

lncRNA HCG11 Promotes Colorectal Cancer Cell Malignant Behaviors via Sponging miR-26b-5p

“…In this study, we aimed to fathom out the functional role of HCG11 in the regulation of CRC cell malignant behaviors. Based on previous literature, HCG11 was verified as a tumor suppressor in several malignancies, including gastric cancer [ 31 ] and cervical cancer [ 32 ] while HCG11 was also suggested as an oncogenic factor in ovarian cancer [ 33 ]. In the current study, HCG11 was detected to be aberrantly overexpressed in CRC cell lines, and based on results of functional assays, we confirmed that HCG11 silence could promote the apoptosis of CRC cells while inhibiting cell proliferation, migration, and invasion.…”

“…MALAT1 was corroborated as a ceRNA to exert oncogenic influences on CRC [ 37 ]. Additionally, HCG11 was verified to sponge miR-942-5p to enhance BRMS1 expression, thus affecting the biological behaviors of gastric cancer cells [ 31 ]. Consistent with recent studies, HCG11 was validated as a molecular sponge of miR-26b-5p, targeting ARPP19 to positively modulate ARPP19 expression.…”